Isolation of Methicillin Resistant (MR) Staphylococci from Chicken Meat Samples

Year 2021, Volume: 10 Issue: 2, 126 - 131, 12.12.2021

Özkan Aslantaş , Nejla Yıldırım

https://doi.org/10.31196/huvfd.958632

Cited By: 1

Abstract

The aim of this study was to determine the presence of methicillin resistant staphylococci (MRS) in retail raw chicken meat samples sold in Hatay. The antimicrobial susceptibility of the isolates was tested for 13 different antimicrobials by disc diffusion method and investigated for resistance genes encoding methicillin (mecA), tetracycline (tetM, tetK), penicillin (blaZ), macrolide (ermA, ermC), lincosamide (lnuA) and aminoglycoside [aac(6′)/aph(2′′), aph(3′)‐IIIa, ant(4′)‐Ia] resistance via the use of polymerase chain reaction (PCR). In addition, the presence of staphylococcal enterotoxin (SE) genes was also searched by PCR. Out of 50 collected chicken meat samples, 11 (22%) MRS was isolated and the following species were determined: S. sciuri (72.7%, 8/11) and S. epidermidis (27.3%, 3/11). While all isolates were resistant to oxacillin, penicillin and ampicillin, various rates of resistance were observed for tetracycline (8, 72.7%), clindamycin (3, 27.3%), trimethoprim‐sulfamethoxazole (2, 18.2%), erythromycin (2, 18.2%) and rifampicin (1, 9.1%). All MRS harbored mecA gene together with blaZ. The tetM gene responsible for ribosomal protection was detected in all phenotypically tetracycline resistant isolates. ermC gene in erythromycin resistant isolates and lnuA gene in clindamycin resistant isolates were detected. None of the isolates was found to be positive for SE genes. The results of this study indicated that contamination of retail raw chicken meat samples with MRS poses a risk to public health due to transmission of these bacteria to humans. Additionally, this study also highlights the importance of monitoring antimicrobial resistant bacteria in animal originated foods.

Keywords

Chicken meat, Methicillin resistance, Staphylococci

Supporting Institution

Hatay Mustafa Kemal University

Project Number

21.LÖKAP.001

Thanks

We would like to thank Hatay Mustafa Kemal University Scientific Research Fund for their support in this study.

Tavuk Eti Örneklerinden Metisiline Dirençli (MR) Stafilokok İzolasyonu

Abstract

Bu çalışmanın amacı Hatay'da satılan 50 perakende çiğ tavuk eti örneğinde metisiline dirençli stafilokok (MRS) varlığının araştırılması idi. İzolatların antimikrobiyal duyarlılıkları disk difüzyon yöntemiyle 13 farklı antimikrobiyal için test edildi ve metisilin (mecA), tetrasiklin (tetM, tetK), penisilin (blaZ), makrolid (ermA, ermC), linkozamid (lnuA) ve aminoglikozid [aac(6′)/aph(2′′), aph(3′)‐IIIa, ant(4′)‐Ia] direncine aracılık eden genler yönünden polimeraz zincir reaksiyonu (PCR) ile incelendi. Ayrıca, stafilokokal enterotoksin (SE) genlerinin varlığı da PCR ile araştırıldı. İncelenen 50 perakende çiğ tavuk eti örneğinin 11'inden (%22) MRS izole edildi ve bu izolatların 8'i (%72.7) S. sciuri ve 3'ü (%27.3) de S. epidermidis olarak identifiye edildi. Tüm izolatlar oksasilin, penisilin ve ampisiline dirençli iken tetrasiklin (8, %72.7), klindamisin (3, %27.3), trimetoprim‐sülfametoksazol (2, %18.2), eritromisin (2, %18.2) ve rifampisine (1, %9.1) farklı direnç oranları gözlendi. Tüm MRS izolatları blaZ ile birlikte mecA genini taşıdı. Fenotipik olarak tetrasiklin dirençli izolatlarda ribozomal korumadan sorumlu tetM geni tespit edildi. Eritromisine dirençli izolatlarda ermC geni ve klindamisine dirençli izolatlarda lnuA geni saptandı. İzolatlar SE genlerinin varlığı açısından negatif bulundu. Bu çalışmanın sonuçları, perakende çiğ tavuk örneklerinde MRS'nin varlığının, antibiyotik direncinin insanlara olası yayılması nedeniyle halk sağlığı açısından önemli olabileceğini göstermiştir. Ayrıca bu çalışma, hayvansal kaynaklı gıdalarda antimikrobiyal dirençli bakterilerin izlenmesinin önemini de vurgulamaktadır.

Keywords

Tavuk eti, Metisilin direnci, Stafilokok

Project Number

21.LÖKAP.001

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