Comparison of the Results of Automatic Blood Analyser and Manual Peripheral Smear Method in Total and Differential Leukocyte Count in Goats

Year 2022, Volume: 11 Issue: 1, 93 - 99, 15.05.2022

Gülten Emek Tuna

https://doi.org/10.31196/huvfd.1096972

Abstract

The Abacus Vet 5 (AV5) is a volumetric impedance-based automated haematology analyser that offers a total and 3-part differential (lymphocytes, neutrophils and monocytes) white blood cell (WBC) count in goats. This study aimed to compare the total and differential leukocyte counts (absolute and relative) measured with the AV5 haematology analyser with the results obtained by the manual method from blood smears in goats. It is also aimed to compare the compatibility between the two methods. The blood samples of 40 hair goats (9 healthy and 31 diseased) were analysed by both methods. The relationships between the values were evaluated with a correlation coefficient, and the agreements between the methods were assessed with the Bland-Altman method. The correlation between two methods were excellent for total WBC counts (rho = 0.963, p = 0.000), absolute neutrophils (rho = 0.964, p = 0.000), lymphocytes (rho = 0.928, p = 0.000), a good for the neutrophil percentages (rho = 0.824, p = 0.000), and a weak for absolute monocytes (rho = 0.426, p = 0.006). Although the bias for lymphocyte (−8.25 %) and neutrophil (10.02 %) percentages was relatively significant and the confidence intervals were wide, the agreement for all parameters was acceptable between the two methods in the Bland atman method. In conclusion, the AV5 haematology analyser performed well for total, and differential WBCs counts in goats. However, abnormal results should still be confirmed by a blood smear examination. In addition, instrument performance evaluations, including increased sample sizes, should be performed in further studies.

Keywords

blood smear, goat, haematology analyser, manual method, white blood cell

Thanks

We would like to thank Aleyna DEMİRCİ for their support in this study.

Keçilerde Total ve Diferansiyel Lökosit Sayımlarında Otomatik Kan Analizörü ve Manuel Periferik Yayma Yöntemi Sonuçlarının Karşılaştırılması

Abstract

Abacus Vet 5 (AV5), keçilerde toplam ve 3 parçalı diferansiyel (lenfositler, nötrofiller ve monositler) lökosit (WBC) sayısı sunan hacimsel empedans tabanlı otomatik bir hematoloji analizörüdür. Bu çalışmada, keçilerde AV5 hematoloji analizörü ile ölçülen toplam ve diferansiyel lökosit sayılarının (mutlak ve bağıl), kan yaymalarından manuel yöntemle elde edilen sonuçlarla karşılaştırılması amaçlandı. Ayrıca iki yöntemin uyumluluğunun karşılaştırılması da amaçlanmaktadır. Kırk kıl keçisinin (9 sağlıklı ve 31 hasta) kan örnekleri her iki yöntemle de analiz edildi. Değerler arasındaki ilişkiler korelasyon katsayısı ile, yöntemler arasındaki uyumlar ise Bland-Altman yöntemi ile değerlendirildi. İki yöntem arasındaki korelasyon, toplam WBC sayıları (rho = 0.963, p = 0.000), mutlak nötrofiller (rho = 0.964, p = 0.000) ve lenfositler (rho = 0.928, p = 0.000) için çok iyi, nötrofil yüzdeleri için iyi (rho = 0.824, p = 0.000) ve mutlak monositler için zayıftı (rho = 0.426, p = 0.006). Lenfosit (% -8,25) ve nötrofil (%10,02) yüzdeleri için yanlılık göreceli olarak önemli ve güven aralıkları geniş olmasına rağmen, Bland-Altman yönteminde iki yöntem arasında tüm parametreler için uyum kabul edilebilirdi. Sonuç olarak, AV5 hematoloji analizörü keçilerde toplam ve diferansiyel WBC sayıları için iyi performans göstermiştir. Bununla birlikte, anormal sonuçlar yine de bir kan frotisi incelemesi ile doğrulanmalıdır.

Keywords

hematoloji analiz cihazı, kan frotisi, keçi, lökosit, manuel yöntem

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