Fötal Gelişim Süresince Sığır Karaciğerindeki Sitokeratin 8 ve Sitokeratin 18 Proteinlerinin Dağılımı

Year 2022, Volume: 11 Issue: 2, 225 - 231, 30.12.2022

Uğur Topaloğlu

https://doi.org/10.31196/huvfd.1193894

Abstract

Embriyonik gelişimin kontrolünde kritik rol alan sitokeratinler (CK), embriyogenezis esnasında epitel hücre gelişiminin değişen aşamalarında farklı keratinler şeklinde ekspresse edilir. CK8 ve CK18 proteinleri; çeşitli parankimatöz epitel başta olmak üzere basit epitel hücrelerinin primer keratin çifti olarak bilinmektedirler. Karaciğer; embriyonal dönemde kan yapımı ve kan hacmi regülasyonu, protein sentezi, bağışıklık sistemine katkı, büyüme sinyal yollarının endokrin kontrolü, metabolitleri depolama, safra salgısı ve detoksifikasyon gibi çok sayıda fizyolojik rollere sahiptir. Bu çalışma; CK8 ve CK18 proteinlerinin sığır fötal karaciğer hücrelerindeki dağılım ve lokalizasyonlarının immunohistokimyasal yolla belirlenmesi amacıyla yapılmıştır. Çalışmada özel kesimhanelerden temin edilen 27 adet sığır fetüsü kullanıldı. Yaşları belirlenen fetüsler; gebeliğin birinci (69-89 günlük / 9 fetüs), ikinci (99-178 / 9 fetüs) ve üçüncü (190-269 / 9 fetüs) dönemlerine ait olacak şekilde gruplandırıldı. Fetüslerden alınan karaciğer örnekleri 18 saat boyunca %10’luk formol-alkolde tespit edildi. Rutin histolojik prosedürlerden sonra elde edilen kesitlere immunohistokimyasal boyamalar yapıldı. Boyama sonucunda; CK8 ve CK18’in gebelik süresince safra kanalı epitel hücrelerinde çok güçlü seviyede ekspresse olduğu görüldü. Bununla birlikte hepatositlerde ise CK8 ve CK18’in gebelik dönemlerine göre değişen yoğunluklarda ekspresyon gösterdikleri belirlendi. Böylece CK8 ve CK18'in; sığır karaciğer gelişiminin kontrolü, hepatositlerin ve safra kanal epitel hücrelerinin bölünmesi, çoğalması ve farklılaşmaları gibi birçok role sahip olabilecekleri düşünüldü.

Keywords

Fötal karaciğer, İmmunohistokimya, Sığır, Sitokeratinler, CK8, CK18, Bovine, Cytokeratins, Fetal liver, Immunohistochemistry

Thanks

Bu çalışmadaki bulguların değerlendirilmesinde desteklerini esirgemeyen Doç. Dr. Mehmet Erdem AKBALIK’a teşekkürlerimi sunarım.

Distribution of Cytokeratin 8 and Cytokeratin 18 Proteins in Bovine Liver During Fetal Development

Abstract

Cytokeratins (CK), which play a critical role in the control of embryonic development, are expressed as different keratins at varying stages of epithelial cell development during embryogenesis. The CK8 and CK18 proteins are the primary keratin pair of simple epithelial cells, especially of various parenchymatous epithelium. The liver has many physiological roles, such as blood production and blood volume regulation, protein synthesis, contribution to the immune system, endocrine control of growth signaling pathways, metabolite storage, bile secretion, and detoxification during the embryonal period. This study was conducted to determine the distribution and localization of CK8 and CK18 proteins in bovine fetal liver cells by immunohistochemical method. In the study, 27 bovine fetuses obtained from private slaughterhouses were used. The fetuses whose ages were determined; were grouped as belonging to the first (69-89 days / 9 fetuses), second (99-178 days / 9 fetuses) and third (190-269 days/ 9 fetuses) periods of pregnancy. Then, liver tissue samples were taken from the fetuses and fixed in 10% formol-alcohol for 18 hours. The tissue samples were then subjected to routine histological procedures and immunohistochemistry staining. As a result of staining, it was observed that CK8 and CK18 were strongly expressed in bile duct epithelial cells during pregnancy. However, it has been determined that CK8 and CK18 are expressed in hepatocytes with varying densities according to the pregnancy periods. Thus, it was thought that CK8 and CK18 might have many roles, such as controlling the development of the bovine liver, division, proliferation, and differentiation of hepatocytes and bile duct epithelial cells.

Keywords

Bovine, Cytokeratins, Fetal liver, Immunohistochemistry, CK8, CK18

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