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Sığırların Genital Mikoplazma ve Üreplazma İnfeksiyonlarının PCR ile Moleküler Teşhisi

Year 2023, Volume: 12 Issue: 1, 21 - 26, 22.06.2023
https://doi.org/10.31196/huvfd.1194267

Abstract

Genital sistem kommensalleri olan genital mikoplazmalar (Mycoplasma spp. ve Ureaplasma diversum) büyükbaş hayvan işletmelerinde özellikle çiftlik düzeyinde üreme yetersizliği ile ekonomik kayıplara neden olmaktadır. Verimlilik kaybının başlıca nedenleri genetik ve çevresel faktörler, stres, yanlış antibiyotik tedavisi ve bağışıklık sistemi yetmezliği ile ilişkilidir. Bu çalışmanın amacı, sağlıklı ve infertil olduğundan şüphelenilen sığırlarda sığır genital mikoplazma ve üreplazmaların varlığının ilk defa PCR ile belirlenmesidir. Test sonuçları ayrıca geleneksel yöntemlerle de incelenmiştir. Test edilen her iki yöntemde de sonuçların bir karşılaştırması yapılmıştır. Bu amaçla, Ankara ve Türkiye'nin illerinden çeşitli sürülere ait sağlıklı ve kısır olduğundan şüphelenilen inekler ve boğalardan toplam 356 adet sığır genital örneği toplandı. 221 sağlıklı ve 135 şüpheli infertil sığırın genital materyalinde pozitiflik sırasıyla kültürel yöntemlerle % 61,2 ve PCR ile % 67,7 olarak bulundu. Kültüre göre PCR daha kısa sürede doğru sonuçlar vermesine rağmen bulgular her iki yöntemin birlikte kullanılması durumunda uygun sonuçların alınabileceğini gösterdi.

References

  • 1. Referans1 ABELE-HORN, M., Blendinger, C., Becher, C., Emmerling,P., Ruckdeschel, G. (1996). Evaluation of commercial kits for quantitative identification and tests on antibiotic susceptibility of genital mycoplasmas. Zentralbl, Bakteriol.,284(4):540-9.
  • 2. Referans2Cardoso VM, Blanchard A, Ferris S, Timenetsky VR, Cunha RA. 2000a:. Detection of Ureaplasma diversum in cattle using a newly developed PCR-based detection assay. Vet Microbiol, 72, 241-50.
  • 3. Referans3 CARDOSO, M.V., SCARCELLI, E., GRASSO,L. M., TEIXEIRA, S.R., GENOVEZ, M. E. (2000b).Ureaplasma diversum and reproductive disorder in Brazilian cows and heifers; first report. Anim. Reprod. Sci., 63: 137-143.
  • 4. Referans4 CASSELL, G.H., CROUSE, D. T., WAITES, K. B., RUDD, P. T., DAVIS, J. K. (1989). Does Ureaplasma urealyticum cause respiratory disease in newborns? Pediat. Infect. Dis., 7:535-541.
  • 5. Referans5 CSAIKLI, U.M., BASTIAN, H., BRETTSCHNEIDER, R., GAUCH, S., MEIR, A., SCHAUERTE, M., SCHOLZ, F., SPERISEN, C., VORNAM, B., ZIEGENHAGEN, B. ( 1998). Comparative analysis of different DNA extractions protocols: A fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies. Plant Mol. Bio. Rep., 16: 69–86.
  • 6. Referans6 ERNO, H., PLASTRIDGE, W. N., TOUTELLOTTE, M. E. (1967). Mycoplasma. Isolation from prepuce and semen of bulls. Acta Vet. Scand., 8: 123-135.
  • 7. Referans7 GENOVEZ, M.E., SCARECLLI, E., ROJAS, S. (1989). Campilobacteriose genital:proposta de diagnostico mais sensivel em touros, Sao Paulo. Arq.Inst. Biolo., 56: 5-7.
  • 8. Referans8 GREGORY,L., Rizzo, H., Gaeta, N.C. , Tortorelli, G. , Cardoso, M.V. , Mettifogo, E. , Buzinhani, M. , Timenetsky, J. (2012). Interference of Mycoplasma spp. or Ureaplasma spp. in ovine semen quality. J. of Microbiol. Res., 2(5): 118-122.
  • 9. Referans9 KOBAYASHI, H., HIROSE, K., WORARACH, A., PAUGTES, P., ITO, N., MOROZUMI, T., YAMAMOTO, K. (1998). In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. J. Vet. Med. Sci., 60 : 1299-1303.
  • 10. Referans10 LEIN, D.H. (1986). The current role of ureaplasma, mycoplasma, Haemophilus somnus in bovine reproductive disorders. Proceedings of the Eleventh Technical Conference on Artificial Insemination and Reproduction. 11: 27-32.
  • 11. Referans11 MURRAY, M.G., THOMPSON, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res., 8: 4321.
  • 12. Referans12 RAZIN, S., Yogev, D., Naot, Y. (1998). Molecular Biology and Pathogenicity of Mycoplasmas. Microbiol. Mol. Biol.Rev., 62(4): 1094–1156.
  • 13. Referans13 RAZIN, S. (2002). Molecular Biology and Pathogenicity of Mycoplasmas. Edited by Razin. Chapter: 24, p.: 538., Kluwer Academic Publishers, New York.
  • 14. Referans14 SUBRAMANIAM, S., BERGONIER, D., POUMARAT, F., CAPAUL, S., SCHLATTER, Y., NICOLET, J., FREY, J. (1998). Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol. Cell Prob., 12: 161- 169.

Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR

Year 2023, Volume: 12 Issue: 1, 21 - 26, 22.06.2023
https://doi.org/10.31196/huvfd.1194267

Abstract

Genital Mycoplasmas (Mycoplasma spp. and Ureaplasma diversum), commensals of the genital tract, cause economic loss in cattle enterprises by mainly reproductive failure at the farm level. The productivity loss is due to genetic and environmental factors, stress, wrong antibiotic treatment, and immune system failure. This study aimed to determine the presence of bovine genital mycoplasma and ureaplasmas in healthy and suspected infertile cattle by PCR for the first time. Conventional methods are also used to examine test results. A comparison of results was conducted in both tested methods. For this aim, 356 bovine genital samples of healthy and suspected infertile cows and bulls of various herds were collected from Ankara and the provinces of Türkiye. Positivity in the genital sample of 221 healthy and 135 suspected infertile cattle was 61.2% by cultural methods and 67.7% by PCR, respectively. In comparison to culture, although PCR gave accurate results in a shorter time, findings show that proper results may be obtained when both methods are used together.

References

  • 1. Referans1 ABELE-HORN, M., Blendinger, C., Becher, C., Emmerling,P., Ruckdeschel, G. (1996). Evaluation of commercial kits for quantitative identification and tests on antibiotic susceptibility of genital mycoplasmas. Zentralbl, Bakteriol.,284(4):540-9.
  • 2. Referans2Cardoso VM, Blanchard A, Ferris S, Timenetsky VR, Cunha RA. 2000a:. Detection of Ureaplasma diversum in cattle using a newly developed PCR-based detection assay. Vet Microbiol, 72, 241-50.
  • 3. Referans3 CARDOSO, M.V., SCARCELLI, E., GRASSO,L. M., TEIXEIRA, S.R., GENOVEZ, M. E. (2000b).Ureaplasma diversum and reproductive disorder in Brazilian cows and heifers; first report. Anim. Reprod. Sci., 63: 137-143.
  • 4. Referans4 CASSELL, G.H., CROUSE, D. T., WAITES, K. B., RUDD, P. T., DAVIS, J. K. (1989). Does Ureaplasma urealyticum cause respiratory disease in newborns? Pediat. Infect. Dis., 7:535-541.
  • 5. Referans5 CSAIKLI, U.M., BASTIAN, H., BRETTSCHNEIDER, R., GAUCH, S., MEIR, A., SCHAUERTE, M., SCHOLZ, F., SPERISEN, C., VORNAM, B., ZIEGENHAGEN, B. ( 1998). Comparative analysis of different DNA extractions protocols: A fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies. Plant Mol. Bio. Rep., 16: 69–86.
  • 6. Referans6 ERNO, H., PLASTRIDGE, W. N., TOUTELLOTTE, M. E. (1967). Mycoplasma. Isolation from prepuce and semen of bulls. Acta Vet. Scand., 8: 123-135.
  • 7. Referans7 GENOVEZ, M.E., SCARECLLI, E., ROJAS, S. (1989). Campilobacteriose genital:proposta de diagnostico mais sensivel em touros, Sao Paulo. Arq.Inst. Biolo., 56: 5-7.
  • 8. Referans8 GREGORY,L., Rizzo, H., Gaeta, N.C. , Tortorelli, G. , Cardoso, M.V. , Mettifogo, E. , Buzinhani, M. , Timenetsky, J. (2012). Interference of Mycoplasma spp. or Ureaplasma spp. in ovine semen quality. J. of Microbiol. Res., 2(5): 118-122.
  • 9. Referans9 KOBAYASHI, H., HIROSE, K., WORARACH, A., PAUGTES, P., ITO, N., MOROZUMI, T., YAMAMOTO, K. (1998). In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. J. Vet. Med. Sci., 60 : 1299-1303.
  • 10. Referans10 LEIN, D.H. (1986). The current role of ureaplasma, mycoplasma, Haemophilus somnus in bovine reproductive disorders. Proceedings of the Eleventh Technical Conference on Artificial Insemination and Reproduction. 11: 27-32.
  • 11. Referans11 MURRAY, M.G., THOMPSON, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res., 8: 4321.
  • 12. Referans12 RAZIN, S., Yogev, D., Naot, Y. (1998). Molecular Biology and Pathogenicity of Mycoplasmas. Microbiol. Mol. Biol.Rev., 62(4): 1094–1156.
  • 13. Referans13 RAZIN, S. (2002). Molecular Biology and Pathogenicity of Mycoplasmas. Edited by Razin. Chapter: 24, p.: 538., Kluwer Academic Publishers, New York.
  • 14. Referans14 SUBRAMANIAM, S., BERGONIER, D., POUMARAT, F., CAPAUL, S., SCHLATTER, Y., NICOLET, J., FREY, J. (1998). Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol. Cell Prob., 12: 161- 169.
There are 14 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery, Veterinary Microbiology
Journal Section Research
Authors

Başak Oksay 0000-0002-2484-8001

Kadir Serdar Diker 0000-0003-2150-5553

Early Pub Date June 22, 2023
Publication Date June 22, 2023
Submission Date October 25, 2022
Acceptance Date April 5, 2023
Published in Issue Year 2023 Volume: 12 Issue: 1

Cite

APA Oksay, B., & Diker, K. S. (2023). Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR. Harran Üniversitesi Veteriner Fakültesi Dergisi, 12(1), 21-26. https://doi.org/10.31196/huvfd.1194267
AMA Oksay B, Diker KS. Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR. Harran Univ Vet Fak Derg. June 2023;12(1):21-26. doi:10.31196/huvfd.1194267
Chicago Oksay, Başak, and Kadir Serdar Diker. “Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR”. Harran Üniversitesi Veteriner Fakültesi Dergisi 12, no. 1 (June 2023): 21-26. https://doi.org/10.31196/huvfd.1194267.
EndNote Oksay B, Diker KS (June 1, 2023) Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR. Harran Üniversitesi Veteriner Fakültesi Dergisi 12 1 21–26.
IEEE B. Oksay and K. S. Diker, “Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR”, Harran Univ Vet Fak Derg, vol. 12, no. 1, pp. 21–26, 2023, doi: 10.31196/huvfd.1194267.
ISNAD Oksay, Başak - Diker, Kadir Serdar. “Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR”. Harran Üniversitesi Veteriner Fakültesi Dergisi 12/1 (June 2023), 21-26. https://doi.org/10.31196/huvfd.1194267.
JAMA Oksay B, Diker KS. Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR. Harran Univ Vet Fak Derg. 2023;12:21–26.
MLA Oksay, Başak and Kadir Serdar Diker. “Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR”. Harran Üniversitesi Veteriner Fakültesi Dergisi, vol. 12, no. 1, 2023, pp. 21-26, doi:10.31196/huvfd.1194267.
Vancouver Oksay B, Diker KS. Molecular Diagnosis of Bovine Genital Mycoplasmopsis and Ureaplasma Infections by PCR. Harran Univ Vet Fak Derg. 2023;12(1):21-6.