Monosodyum İyodoasetat ile Oluşturulan Diz Osteoartriti Olan Sıçan Modellerinin Tedavisinde Kullanılan Borik Asitin Karaciğer Dokusu Üzerine Etkilerinin Araştırılması

Year 2023, Volume: 12 Issue: 2, 202 - 208, 22.12.2023

İsmail Bolat , Kübra Asena Terim Kapakin , Esra Manavoğlu Kirman , Gülşah Gündoğdu , Koksal Gundogdu , Fatma Demirkaya Miloğlu , Seymanur Yılmaz Tascı

https://doi.org/10.31196/huvfd.1363620

Cited By: 2

Abstract

Osteoartrit (OA), bireylerin sağlığını birçok sektörde etkileyebilen önemli bir eklem hastalığıdır. OA sağlık alanında öncelikle bir eklem hastalığı olarak bilinse de tetiklediği sistemik inflamatuar reaksiyonlar (SIRS) nedeniyle karaciğer gibi organlarda da hasara yol açabilmektedir. Birçok çalışmaya konu olan ve anti-enflamatuar, anti-apoptotik, anti-mikrobiyal ve anti-kanser özelliklere sahip olduğu gösterilen borik asit (BA), sağlık sektörünün çeşitli alanlarında yaygın olarak kullanılmaktadır. Bu amaçla yapılan bu çalışmada, BA'nın OA'nın neden olduğu karaciğer hasarına karşı koruyucu etkisi araştırılmıştır. Toplam 28 rat, her biri 7 rattan oluşan dört gruba ayrılmıştır. Kontrol grubu hariç, tüm hayvanlar monosodyum iyodoasetat (MIA) ile indüklenerek OA geliştirilmiş ve ardından ratlara 21 gün boyunca 4 ve 10 mg/kg dozlarda BA uygulanmıştır. Histopatolojik incelemelerde, OA oluşturulan grubun karaciğer dokusunda şiddetli hepatit ve dejeneratif değişiklikler gözlenirken, BA uygulamasının bu hasarları doza bağlı olarak azalttığı tespit edilmiştir. Benzer şekilde, immünohistokimyasal analizlerde, OA indüksiyonunun karaciğerde TNF-α, IL-1β, iNOS ve MMP-13 ekspresyon seviyelerini önemli ölçüde artırdığı, ancak BA ile tedavi edilen gruplarda bu ekspresyon seviyesinin doza bağlı olarak önemli ölçüde azaldığı gözlenmiştir. Bu çalışma, BA'nın anti-inflamatuar özellikler sergilediğini ve OA'nın neden olduğu SIRS'e karşı karaciğer dokusu üzerinde koruyucu bir etki gösterdiğini gözlemledi.

Keywords

Borik asit, histopatoloji, immünohistokimya, Monosodium iodoacetate, Osteoartritis

Investigation of the Effects of Boric Acid Used in the Treatment of Rat Models with Knee Osteoarthritis Induced by Monosodium Iodoacetate on Liver Tissue

Abstract

Osteoarthritis (OA) is a significant joint disease that can affect the health of individuals in many sectors. Although OA is primarily known as a joint disease in the field of health, it can also cause damage to organs such as the liver due to the systemic inflammatory reactions (SIRS) it triggers. Boric acid (BA), which has been the subject of many studies and has been shown to have anti-inflammatory, anti-apoptotic, antimicrobial, and anticancer properties, is widely used in various areas of the healthcare sector. In this study conducted for this purpose, the protective effect of BA against liver damage caused by OA was investigated. A total of 28 rats were divided into four groups, each consisting of 7 rats. Except for the control group, all animals were induced with monosodium iodoacetate (MIA) to develop OA, and then rats were administered doses of BA ranging 4 and 10 mg/kg for 21 days. In histopathological examinations, severe hepatitis and degenerative changes were observed in the liver tissue of the OA-induced group, while it was found that BA application reduced these damages dose-dependently. Similarly, in immunohistochemical analyses, it was observed that OA induction significantly increased the expression levels of TNF-α, IL-1β, iNOS, and MMP-13 in the liver, but in the groups treated with BA, this expression level significantly decreased depending on the dose. This study observed that BA exhibited anti-inflammatory properties and had a protective effect on liver tissue against SIRS caused by OA.

Keywords

Boric acid, Histopathology, Immunohistochemistry, Monosodium iodoacetate, Osteoarthritis

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