The effect of PGF2α injection in different times on the pregnancy rate in progesterone-based synchronization in Holstein cows

Year 2024, Volume: 13 Issue: 2, 148 - 153, 18.12.2024

İbrahim Şahan , Cihan Kaçar , Semra Kaya , Mushap Kuru , Murat Can Demir , Muhammet Ali Karadağ , Canan Gülmez Samsa

https://doi.org/10.31196/huvfd.1489406

Abstract

This study investigated the effect of Prostaglandin F2α (PGF2α) injection at different times on pregnancy rate (PR) in Holstein cows undergoing progesterone-based fixed-time artificial insemination (FTAI). Group 1 (G1, n=30): A progesterone releasing intravaginal device (PRID) was placed intravaginally with a gonadotropin-releasing hormone (GnRH) injection on day 0. Eight days later, PGF2α was injected. PRID was removed on the 9th day, and after 60 hours, FTAI was performed with a GnRH injection. Group 2 (G2, n=30): Unlike G1, PGF2α was injected twice, with an interval of 24 hours, 8 and 9 days after the intravaginal placement of PRID. Group 3 (G3, n=30): PGF2α was injected 9 days after the intravaginal placement of PRID. Pregnancy diagnoses were performed by transrectal ultrasonography 45 days after FTAI. Blood samples were taken on the 8th day (for Groups 1 and 2), the 9th day (for Groups 2 and 3, PRID removal day), and on the day of FTAI (for Groups 1, 2 and 3) and serum progesterone (P4) concentration was determined. The pregnancy rate (PR) was 36.67%, 53.33%, and 43.33% in Groups 1, 2, and 3, respectively (P=0.194). In cows with a body condition score (BCS) <3, repeated PGF2α injections within a 24-hour interval increased the PR (G2: 70%) compared to the other two groups (G1: 20%, G3: 25%) (P=0.045). P4 concentrations decreased after PGF2α injections (P<0.001). In cows experiencing luteolysis, a significant difference was found in PR between G1 and G3 (P=0.04). In conclusion, PGF2α injection at 24-hour intervals in P4-based FTAI protocols in cows quantitatively increased both pregnancy and luteolysis rates and may improve fertility.

Keywords

Cow, progesterone, PGF2α, luteolysis, pregnancy

Holstein ineklerinde progesteron bazlı senkronizasyonda farklı zamanlarda PGF2α enjeksiyonunun gebelik oranına etkisi

Abstract

Bu çalışmada, progesteron bazlı sabit zamanlı suni tohumlama (FTAI) uygulanan Holştayn ineklerde farklı zamanlarda Prostaglandin F2α (PGF2α) enjeksiyonunun gebelik oranı üzerine etkisi araştırıldı. Grup 1 (G1, n=30): Progesteron salgılayan intravajinal cihaz (PRID) 0. günde vajinaya yerleştirildi ve gonadotropin salgılatıcı hormon (GnRH) enjeksiyonu yapıldı. Sekiz gün sonra PGF2α enjekte edildi. PRID, 9. günde çıkarıldı ve 60 saat sonra GnRH enjeksiyonu ile FTAI gerçekleştirildi. Grup 2 (G2, n=30): Grup 1'den farklı olarak PGF2α, sabit zamanlı suni tohumlama (FTAI) intravajinal yerleştirilmesinden 8 ve 9 gün sonra, aralarında 24 saatlik bir süre bulunarak iki kez enjekte edildi. Grup 3 (G3, n=30): PGF2α, PRID'in intravajinal yerleştirilmesinden 9 gün sonra enjekte edildi. Gebelik teşhisleri FTAI'den 45 gün sonra transrektal ultrasonografi ile yapıldı. Kan örnekleri, 8. gün (G1 ve G2 için), 9. gün (PRID çıkarılma günü) ve FTAI gününde alındı ve serum progesteron (P4) konsantrasyonu belirlendi. Gebelik oranları Grup 1, 2 ve 3'te sırasıyla %36.67, %53.33 ve %43.33 olarak bulundu (P=0.194). Vücut kondisyon skoru (BCS) <3 olan ineklerde, 24 saat arayla tekrarlanan PGF2α enjeksiyonları gebelik oranını (G2: %70) diğer iki grupla (G1: %20, G3: %25) karşılaştırıldığında artırdı (P=0.045). P4 konsantrasyonları PGF2α enjeksiyonlarından sonra azaldı (P<0.001). Luteolizis uygulanan ineklerde Grup 1 ve Grup 3 arasında gebelik oranlarında anlamlı fark bulundu (P=0.04). Sonuç olarak, ineklerde P4 bazlı FTAI protokollerinde 24 saatlik aralıklarla PGF2α enjeksiyonunun gebelik ve lüteoliz oranlarını nicel olarak artırdığı ve fertiliteyi iyileştirebileceği sonucuna varıldı.

Keywords

İnek, Progesteron, PGF2α, luteolizis, gebelik

Ethical Statement

Yazarlar, makalenin veya herhangi bir bölümünün başka bir yerde yayınlanmadığını veya yayınlanmasının düşünülmediğini beyan ederler. Yazarlardan hiçbiri yazıda yazar olarak isminin belirtilmesinden doğabilecek herhangi bir çıkar çatışmasını beyan etmemiştir.

Supporting Institution

Bu çalışmada herhangi bir mali kaynak kullanılmamıştır.

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