Balb/c farelerinde meme içi lipopolisakkarid uygulaması sonucu yeni bir kronik mastitis modelin indüklenmesi

Year 2024, Volume: 13 Issue: 2, 113 - 117, 18.12.2024

Özkan Yavaş , Ahmet Akkoç

https://doi.org/10.31196/huvfd.1530358

Abstract

Mastitis, meme dokusunun iltihaplanmasıdır ve dünya genelinde çiftlik hayvanlarında yaygın olarak görülmektedir. Bu sorun süt endüstrisinde veteriner masrafları, sütün imhası ve tedavi giderleri açısından ciddi mali kayıplara neden olmaktadır. Bakteriler etiyolojinin ana aktörleridir ve meme dokusunda akut ve kronik inflamatuar değişikliklere neden olurlar. Akut enflamatuar değişiklikler klinik olarak kolayca tanınır ve tedavi hemen başlatılır, ancak subakut inflamasyon sinsice ilerler ve geri dönüşü olmayan fibrotik değişikliklerle kronik inflamasyona yol açar. Laboratuvar hayvanlarında akut mastitis indüksiyonu için standartlaştırılmış deneysel modeller mevcuttur ve genellikle bakterilerin veya bazı bakteriyel yapısal bileşenlerin meme dokusuna infüzyonu bu amaçla kolayca uygulanmaktadır. Bununla birlikte, fibrotik değişikliklerle birlikte kronik mastitis indüksiyonu üzerine az sayıda çalışma vardır ve uygulamalar nispeten karmaşıktır. Sunulan çalışmada, farelerde kronik mastitisi indüklemek için LPS 0, 5 ve 10. günlerde üç kez meme kanalından infüze edilmiştir. Histopatolojik değişiklikleri değerlendirmek için 1, 6 ve 15. günlerde dokulardan örnek alınmıştır. Akut inflamasyonun bir bileşeni olan şiddetli nötrofil infiltratları 1. günde gözlenirken, lenfosit infiltratları subakut inflamasyonla uyumlu olarak 6. günde artmıştır. 15. günde, fibrozis ve lenfosit infiltrasyonu gibi kronik mastitisi temsil eden lezyonlar gözlenmiştir. Süt sığırlarının kronik mastitisindeki lezyonlara benzer bir model, farelerde LPS infüzyonu ile başarılı ve kolay bir şekilde oluşturulmuştur.

Keywords

Balb/c, Kronik mastitis modeli, Lipopolisakkarit, Meme bezi, Masson Trikrom.

Project Number

118O893

A new protocol for the induction of chronic mastitis with intramammary infusion of lipopolysaccharide (LPS) in Balb/c mice

Abstract

Mastitis is inflammation of the mammary tissue and is commonly observed in farm animals. The problem causes severe financial losses in the dairy industry in terms of veterinary costs, milk disposal, and treatment expenses. Bacteria are the main actors in the etiology and cause acute and chronic inflammatory changes in the mammary tissue. Acute inflammatory changes are easily recognized clinically, and treatment is initiated immediately, but subacute inflammation progresses insidiously and leads to chronic inflammation with irreversible fibrotic changes. Standardized experimental models for the induction of acute mastitis in laboratory animals are available. Usually, infusion of bacteria or some bacterial structural components into mammary tissue is easily applied for this purpose. However, there are few studies on the induction of chronic mastitis with fibrotic changes, and the applications are relatively complex. In this study, LPS was infused through the teat duct three times on days 0, 5, and 10 to induce chronic mastitis in mice. Tissues were sampled on days 1, 6, and 15 to evaluate histopathological changes. While severe neutrophil infiltrates, a component of acute inflammation, were observed on day 1, lymphocyte infiltrates increased on day 6, consistent with subacute inflammation. On day 15, lesions representing chronic mastitis, such as fibrosis and lymphocyte infiltration, were observed. A model similar to the lesions in chronic mastitis of dairy cattle was successfully and easily established by LPS infusion in mice.

Keywords

Balb/c, LPS, Masson’s Trichrome, Masson’s Trichrome, Masson’s Trichrome

Ethical Statement

This study was approved by the Bursa Uludag University Animal Experiments Local Ethics Committee (2019/07-04 Number Ethics Committee Decision). In addition, the authors declared that Research and Publication Ethical rules were followed.

Supporting Institution

TÜBİTAK

Project Number

118O893

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