The Role of Some Selenoproteins in the Etiopathogenesis of Breast Cancer

Abstract

Aim: Breast cancer is the second leading cause of cancer-related deaths in women, after lung cancer. In various studies, it has been shown that selenoproteins suppress some stages of carcinogenesis and decrease the proliferation rate of cancer cells. However, these mechanisms have not been fully elucidated. Radiotherapy is one of the most preferred treatment methods along with chemotherapy in cancer treatment. This study aims to evaluate the changes in the pre- and post-treatment selenoprotein levels of breast cancer patients who received radiotherapy and to examine the effects on the etiopathogenesis of the disease.

Method: A total of 35 woman breast cancer patients and 25 healthy subjects were included in the study. Blood samples were collected from the patient group on the day prior to treatment, and on the day treatment was completed. Selenoprotein K (Sel-K), selenoprotein W1 (Sel-W1) and selenoprotein P (Sel-P) levels were measured in serum samples by ELISA (Enzyme-Linked Immunosorbent Assay) method. Statistical analysis was performed using Wilcoxon and Mann-Whitney U tests. Statistical Package for the Social Sciences – SPSS 21.0 for Windows (SPSS Inc, Chicago, IL, USA) was used for calculations. P<0.05 was accepted to indicate a statistically significant difference.

Results: Serum Sel-K levels were significantly lower in the pre-treatment group compared to the control group. When Sel-P levels in both pre- and post-treatment were compared with the control group, it was found lower Sel-P levels in both treatment groups compared to the control group. There was no significant difference between groups in Sel-W1 levels between studied groups.

Conclusions: Although some selenoproteins have an important role in the etiopathogenesis of breast cancer, more sample groups and further studies are needed to investigate the relationship between disease progression and selenoprotein levels.

Keywords

• breast cancer
• selenoprotein
• treatment

Project Number

TDK-2022-38056

Meme Kanserinin Etiyopatogenezinde Bazı Selenoproteinlerin Rolü

Abstract

Amaç: Meme kanseri, kadınlarda kanser kaynaklı ölümlerde akciğer kanserinden sonra ikinci sırada yer alır. Çeşitli çalışmalarda, selenoproteinlerin kanserogenezin bazı evrelerini baskıladığı ve kanser hücrelerinin çoğalma hızını azalttığı gösterilmiştir. Ancak bu mekanizmalar tam olarak açıklanamamıştır. Kanser tedavisinde radyoterapi, kemoterapiyle birlikte en çok tercih edilen tedavi yöntemlerindendir. Çalışmanın amacı, radyoterapi alan meme kanserli hastaların tedavi öncesi ve sonrası selenoprotein düzeylerindeki değişiklikleri değerlendirerek hastalığın etiyopatogenezine olası etkilerini incelemektir.

Yöntem: Çalışmamıza meme kanseri teşhisi konmuş, radyoterapi öncesi ve radyoterapi sonrası örnekleri alınan 35 kadın hasta ile herhangi bir ilaç tedavisi almayan 25 sağlıklı kadın gönüllü dahil edildi. Hasta ve sağlıklı kontrol gruplarını oluşturan bireylerden kan örnekleri alındı. Serum örneklerinde selenoprotein K (Sel-K), selenoprotein W1 (Sel-W1) ve selenoprotein P (Sel-P) düzeyleri ELISA (Enzyme-Linked Immunosorbent Assay) yöntemi ile ölçüldü. İstatistiksel analiz, Wilcoxon ve Mann-Whitney U testleri kullanılarak yapıldı. Hesaplamalar için Statistical Package for the Social Sciences – SPSS 21.0 for Windows (SPSS Inc, Chicago, IL, ABD) kullanıldı. p<0.05, istatistiksel olarak anlamlı bir farkı belirtmek için kabul edildi.

Bulgular: Serum Sel-K düzeyleri tedavi öncesi ve kontrol grubu karşılaştırıldığında, tedavi öncesi grupta anlamlı olarak düşük bulundu. Sel- P düzeyleri hem tedavi öncesi hem de tedavi sonrasında kontrol grubu ile karşılaştırıldığında her iki grupta da kontrol grubuna göre düşük bulundu. Sel-W1 düzeylerinde gruplar arasında herhangi bir anlamlılık bulunmadı.

Sonuç: Meme kanserinde bazı selenoproteinlerin hastalığın etiyopatogenezinde önemli bir rolü olmakla birlikte daha fazla örneklem grubu ve ileri çalışmalar ile hastalığın progresyonu ve selenoprotein düzeyleri arasındaki ilişkinin araştırılmasına ihtiyaç duyulmaktadır.

Keywords

• meme kanseri
• selenoprotein
• tedavi

Supporting Institution

İstanbul Üniversitesi Bilimsel Projeler Birimi ve Sağlık Bilimleri Üniversitesi Bakırköy Sadi Konuk Eğitim ve Araştırma Hastanesi Araştırma Fonu tarafından desteklenmiştir.

Project Number

TDK-2022-38056

Thanks

Bu çalışma kısmen İstanbul Üniversitesi Bilimsel Projeler Birimi (Proje No: TDK-2022-38056)ve Sağlık Bilimleri Üniversitesi Bakırköy Sadi Konuk Eğitim ve Araştırma Hastanesi Araştırma Fonu tarafından desteklenmiştir.

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