Potential Protective Role of Radicut in Valproic Acid-Induced Oxidative Stress in Rat Spleen

Abstract

Aim: The study aims to evaluate the potential protective role of Radicut (RAD) in Valproic acid (VPA)-induced oxidative stress in splenic tissues of rats. Method: Rats were divided into groups as follows: Group 1: Controls (n=8), Group II: R: RAD-given group (30 mg/kg/day, n=8), Group III: V: VPA-given group (0.5 g/kg/day, n=10), Group IV: V+R: VPA+RAD-given group (30 mg/kg/day, n=11). VPA, RAD, and VPA+RAD were given to the animals for 7 days (i.p). Biochemical parameters related to oxidative stress were determined in spleen homogenates. Results: VPA elevated oxidative stress by increasing lipid peroxidation and sialic acid levels, increasing alkaline phosphatase activity, and decreasing superoxide dismutase, glutathione-S- transferase, and glutathione peroxidase activities. Administration of RAD to VPA-given group decreased LPO, SA levels, and acid phosphatase levels, and increased tissue factor, SOD, GST, and GPx activities. Conclusion: RAD reversed the biochemical results in the V group, by clarifying its protective effect. RAD has the potential to prevent oxidative stress during VPA treatment, which could be beneficial.

Keywords

• Radicut
• valproic acid
• oxidative stress
• antioxidants
• spleen
• rat

Radicut'ın Sıçan Dalağındaki Valproik Asite Bağlı Oksidatif Streste Potansiyel Koruyucu Rolü

Öz

Amaç: Çalışma, sıçanların dalak dokularında Valproik asitin (VPA) neden olduğu oksidatif streste Radikutun (RAD) potansiyel koruyucu rolünü değerlendirmeyi amaçlamaktadır. Yöntem: Sıçanlar belirtildiği gibi gruplara ayrılmıştır: Grup 1: Kontrol (n=8), Grup II: R: RAD verilen grup (30 mg/kg/gün, n=8), Grup III: V: VPA verilen grup (0,5 g/kg/gün, n=10), Grup IV: V+R: VPA+ RAD verilen grup (30 mg/kg/gün, n=11). Hayvanlara 7 gün süreyle (i.p) VPA, RAD ve VPA+RAD verildi. Oksidatif stresle ilgili biyokimyasal parametreler dalak homojenatlarında tayin edildi. Bulgular: VPA, dalakta lipid peroksidasyon ve siyalik asit seviyelerini yükselterek, alkalen fosfataz aktivitesini artırarak ve süperoksit dismutaz, glutatyon s transferaz ve glutatyon peroxidaz aktivitelerini azaltarak oksidatif stresi yükseltmiştir. VPA verilen gruba RAD uygulanması, LPO, SA ve asit fosfataz düzeylerini azaltırken, doku faktörü, SOD, GST ve GPx aktivitelerini artırdı. Sonuç: RAD, koruyucu etkisini göstererek VPA verilen gruptaki biyokimyasal sonuçları normale çevirdi. RAD, VPA tedavisi sırasında yararlı olabilecek oksidatif stresi önleme potansiyeline sahiptir.

Anahtar Kelimeler

• Radikut
• valproik asit
• oksidatif stres
• antioksidanlar
• dalak
• sıçan

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