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Yeni Bir Rinosinüzit Modeli Geliştirmek: Bir Hayvan Deneyi

Year 2023, Issue: 20, 419 - 430, 09.09.2023
https://doi.org/10.38079/igusabder.1288982

Abstract

Amaç: Yeni bir akut bakteriyel rinosinüzit modeli geliştirmektir.
Yöntem: Çalışmada kobay hayvanlar dört gruba ayrılmıştır. Birinci gruptaki hayvanların sağ nazal kavitelerine Streptococcus pneumoniae süspansiyonu damlatılmıştır. İkinci grupta, steril serum emdirilmiş merosel şeritler hayvanların sağ nazal pasajlarına pasajı kapatacak şekilde yerleştirilmiştir. Üçüncü grupta, Streptococcus pneumoniae emdirilmiş merosel şeritler hayvanların sağ nazal kavitelerine yerleştirilmiştir. Dördüncü grup ise herhangi bir girişim veya işlem yapılmayan hayvanların bulunduğu kontrol grubudur. Girişimleri takiben 7., 14., 28., 35. günlerde intranazal kültürler incelenmiş, bilgisayarlı tomografi (CT) görüntüleri tüm kobay hayvanlarından alınmış, ve radyoloji olarak rinosinüsit tespit edilen hayvanlardan iki tanesi rastlantısal olarak seçilerek her hafta dekapite edilmiştir. Sakrifiye edilen kobayların sinüsleri ve nazal spesimenleri histopatolojik olarak incelenmiştir.
Bulgular: Histopatolojik ve radyolojik incelemelerin yapıldığı nazal örneklemeler inflamatuar reaksiyonun ciddiyetini gözlemlemeyi sağlamıştır. Akut bakteriyel rinosinüzit, kontrol grubu hariç tüm gruplardaki kobaylarda indüklenmiştir. Üçüncü gruptaki kobaylarda ilk iki gruba nazaran daha ciddi bir inflamasyon gözlenmiştir. Kontrol grubunda herhangi bir inflamatuar reaksiyon bulunmamıştır. Ayrıca kobay hayvanlarının normal florası da incelenmiştir.
Sonuç: Kobay hayvanı deneysel olarak rinosinüzit oluşturmak için çok uygun bir hayvan deneyi modelidir. Kobay hayvanı cerrahi manipülasyondaki kısıtlılığa rağmen, ilerdeki potansiyel genetik ve inflamasyon çalışmaları için alternatif bir model olacaktır.

References

  • Hilding AH. Experimental sinus surgery; some experiments on ventilation and sinusitis. Laryngoscope. 1948;58(10):1098-1102.
  • Kelemen G. The nasal and paranasal cavities of the rabbit in experimental work. AMA Arch Otolaryngol. 1955;61(5):497-512.
  • Maeyama T. A study of experimental sinusitis in rabbits. Auris Nasus Larynx. 1981;8(2):87-97.
  • Bomer K, Brichta A, Baroody F, Boonlayangoor S, Li X, Naclerio RM. A Mouse model of acute bacterial rhino sinusitis. Arch Otolaryngol Head Neck Surg. 1998;124(11):1227-32.
  • Jacob A, Faddis BT, Chole RA. Chronic bacterial rhino sinusitis: Description of a mouse model. Arch Otolaryngol Head Neck Surg. 2001;127(6):657-64.
  • Jeon SY, Kim JP, Kim EA, Ahn SK, Kim BG. Rat model of platelet-activating factor- induced rhino sinusitis. Ann Otol Rhinol Laryngol. 2005;114(5):393-8.
  • Zhang F, An Y, Li Z, Zhao C. A novel model of invasive fungal rhino sinusitis in rats. Am J Rhinol Allergy. 2013;27(5):361-6.
  • Ramadan HH, Meek RB, Dawson GS, Spirou GA, Cuff CF, Berrebi AS. Histologic and immunologic observations of viral-induced rhino sinusitis in the mouse. Am J Rhinol. 2002;16(1):61-7.
  • Liang KL, Jiang RS, Wang RC, et al. Upper airway inflammation exacerbates bronchial hyperactivity in mouse models of rhino sinusitis and allergic asthma. Int Forum Allergy Rhinol. 2013;3(7):532-42.
  • Drettner B, Johansson P, Kumlien J. Experimental acute sinusitis in rabbit. A study of mucosal blood flow. Acta Otolaryngol. 1987;103:432-4.
  • Phillips JE, Ji L, Rivelli MA, Chapman RW, Corboz MR. Three-dimensional analysis of rodent paranasal sinus cavities from X-ray computed tomography (CT) scans. Can J Vet Res. 2009;73(3):205-11.
  • Marks SC. Acute sinusitis in the rabbit: A new rhinogenic model. Laryngoscope. 1997;107:1579–1585.
  • Dufour X, Kauffmann-Lacroix C, Goujon JM, Grollier G, Rodier MH, Klossek JM. Experimental model of fungal sinusitis: A pilot study in rabbits. Ann Otol Rhinol Laryngol. 2005;114:167–172.
  • Kim HS, Jeon SY, Ahn SK, et al. A rat model of acute bacterial rhino sinusitis induced by Staphylococcus aureus. Korean J Otolaryngol-Head Neck Surg. 2005;48(6):735-740.
  • Khalmuratova R. A rat model of acute rhino sinusitis induced by alphatoxin of Staphylococcus aureus [dissertation]. Chinju: Gyeong-Sang National University; 2006.
  • Kara CO, Cetin CB, Colakoğlu N, Sengül M, Pakdemirli E. Experimentally induced rhino sinusitis in rabbits. J Otolaryngol. 2002;31(5):294-8.

Developing A New Model for Rhinosinusitis: Animal Experiment

Year 2023, Issue: 20, 419 - 430, 09.09.2023
https://doi.org/10.38079/igusabder.1288982

Abstract

Aim: To develop a new model of acute bacterial rhinosinusitis.
Method: We divided guinea pigs into four groups in this study. In the primary group of guinea pigs, right nasal cavities of the animals were vaccinated with Streptococcus pneumoniae suspension. In the second group, sponge strips saturated with sterile saline into the right nasal cavities of animals. In the third group, sponge strips saturated with Streptococcus pneumoniae suspension in the right nasal cavities of animals. The fourth group was control group, which was exposed to no intervention. After 7, 14, 28, 35 days from interventions, intranasal cultures were obtained, computed tomography (CT) scans were imaged from all of the guinea pigs, and two randomly selected guinea pigs that were detected as having rhinosinusitis radiologically were killed every week. The sinuses and nasal specimens of sacrificed guinea pigs were prepared for histopathological investigation.
Results: Radiological and histopathological examinations of nasal samples were performed to observe the severity of the inflammatory reaction. Acute bacterial rhinosinusitis was induced in all groups of subject animals except the control group. More severe inflammation was seen in the third group of subject animals compared to the first and second groups. No inflammatory reaction was found in the control group. We also evaluated the normal flora of guinea pigs.
Conclusion: The guinea pig is certainly a true model for developing rhinosinusitis. Guinea pigs should be considered as an alternative model for further potential studies of genetics and inflammation, even though surgical manipulation is limited.

References

  • Hilding AH. Experimental sinus surgery; some experiments on ventilation and sinusitis. Laryngoscope. 1948;58(10):1098-1102.
  • Kelemen G. The nasal and paranasal cavities of the rabbit in experimental work. AMA Arch Otolaryngol. 1955;61(5):497-512.
  • Maeyama T. A study of experimental sinusitis in rabbits. Auris Nasus Larynx. 1981;8(2):87-97.
  • Bomer K, Brichta A, Baroody F, Boonlayangoor S, Li X, Naclerio RM. A Mouse model of acute bacterial rhino sinusitis. Arch Otolaryngol Head Neck Surg. 1998;124(11):1227-32.
  • Jacob A, Faddis BT, Chole RA. Chronic bacterial rhino sinusitis: Description of a mouse model. Arch Otolaryngol Head Neck Surg. 2001;127(6):657-64.
  • Jeon SY, Kim JP, Kim EA, Ahn SK, Kim BG. Rat model of platelet-activating factor- induced rhino sinusitis. Ann Otol Rhinol Laryngol. 2005;114(5):393-8.
  • Zhang F, An Y, Li Z, Zhao C. A novel model of invasive fungal rhino sinusitis in rats. Am J Rhinol Allergy. 2013;27(5):361-6.
  • Ramadan HH, Meek RB, Dawson GS, Spirou GA, Cuff CF, Berrebi AS. Histologic and immunologic observations of viral-induced rhino sinusitis in the mouse. Am J Rhinol. 2002;16(1):61-7.
  • Liang KL, Jiang RS, Wang RC, et al. Upper airway inflammation exacerbates bronchial hyperactivity in mouse models of rhino sinusitis and allergic asthma. Int Forum Allergy Rhinol. 2013;3(7):532-42.
  • Drettner B, Johansson P, Kumlien J. Experimental acute sinusitis in rabbit. A study of mucosal blood flow. Acta Otolaryngol. 1987;103:432-4.
  • Phillips JE, Ji L, Rivelli MA, Chapman RW, Corboz MR. Three-dimensional analysis of rodent paranasal sinus cavities from X-ray computed tomography (CT) scans. Can J Vet Res. 2009;73(3):205-11.
  • Marks SC. Acute sinusitis in the rabbit: A new rhinogenic model. Laryngoscope. 1997;107:1579–1585.
  • Dufour X, Kauffmann-Lacroix C, Goujon JM, Grollier G, Rodier MH, Klossek JM. Experimental model of fungal sinusitis: A pilot study in rabbits. Ann Otol Rhinol Laryngol. 2005;114:167–172.
  • Kim HS, Jeon SY, Ahn SK, et al. A rat model of acute bacterial rhino sinusitis induced by Staphylococcus aureus. Korean J Otolaryngol-Head Neck Surg. 2005;48(6):735-740.
  • Khalmuratova R. A rat model of acute rhino sinusitis induced by alphatoxin of Staphylococcus aureus [dissertation]. Chinju: Gyeong-Sang National University; 2006.
  • Kara CO, Cetin CB, Colakoğlu N, Sengül M, Pakdemirli E. Experimentally induced rhino sinusitis in rabbits. J Otolaryngol. 2002;31(5):294-8.
There are 16 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Articles
Authors

Başak Çaypınar Eser 0000-0002-3782-4738

Mustafa Salih Canpolat This is me 0000-0001-6163-4769

Pembe Gül Güneş 0000-0002-4982-5156

Arzu İrvem 0000-0003-1307-4538

Sema Zer Toros 0000-0003-3125-732X

Aslı Şahin Yılmaz 0000-0002-7846-9453

Çağatay Oysu 0000-0002-6756-8456

Early Pub Date August 31, 2023
Publication Date September 9, 2023
Acceptance Date July 13, 2023
Published in Issue Year 2023 Issue: 20

Cite

JAMA Çaypınar Eser B, Canpolat MS, Güneş PG, İrvem A, Zer Toros S, Şahin Yılmaz A, Oysu Ç. Developing A New Model for Rhinosinusitis: Animal Experiment. IGUSABDER. 2023;:419–430.

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