A comparative study on solvent extraction effect on the phytochemical profiling and antioxidant capacity of Zizyphus lotus L and Glycyrrhizaglabra L roots extracts from Algeria

Abstract

The aim of this research work is the determination of total phenols, flavonoids, condensed tannin levels, and antioxidant power of methanolic and aqueous extracts of Zizyphus lotus L and Glycyrrhiza glabra L roots, using three methods (Test DPPH, bleaching of β-carotene and hemoglobin denaturation protection). The higher levels of total phenols were found in methanol extracts of G. glabra and Z. lotus (58.94 ± 0.05 and 51.80 ± 0.02 mg GAE/ g of extract, respectively), the methanol extract of Z. lotus was the richest in flavonoids and tannins condensed with the content of 16.00 ± 0.01 and 17.22 ± 0.1 mg CE/g of extract respectively. With the DPPH method, the water extract of Z. lotus represents significantly (p <0.05) the most extract active (04.52 ± 0.17 mg/ml). For bleaching of β-carotene, G. glabra aqueous extract showed significantly (P <0.05) a higher antioxidant activity (71.47±01.86%). The methanol extracts of G. glabra and Z. lotus showed inhibition of hemoglobin denaturation with values of 71.80±0.06 and 71.71±0.33%, respectively. Based on these results, it could be concluded that the G. glabra and Z. lotus roots are a rich source of natural antioxidants of high value.

Keywords

• flavonoid,
• phenolics,
• Zizyphus lotus L,
• Glycyrrhiz aglabra,
• Free Radicals,
• Antioxidant activity,

References

1. Akram, R., Saqib, M., Khalid, B., & Arshad, A. (2015). Effects of Extraction Media and Techniques on the Antioxidant Properties and Recovery of Phenolics from Roots of Glycyrrhiza Glabra. Journal of Molecular Pathophysiology, 4(4), 138. https://doi.org/10.5455/jmp.20151215121630
2. Atmani, D., Chaher, N., Berboucha, M., Ayouni, K., Lounis, H., Boudaoud, H., … Atmani, D. (2009). Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chemistry, 112(2), 303–309. https://doi.org/10.1016/J.FOODCHEM.2008.05.077
3. Bakhtaoui, F. Z., Lakmichi, H., Megraud, F., Chait, A., & Gadhi, C. E. A. (2014). Gastro-protective, anti-Helicobacter pylori and, antioxidant properties of Moroccan Zizyphus lotus L. Journal of Applied Pharmaceutical Science, 4(10), 81–87. https://doi.org/10.7324/JAPS.2014.40115
4. Bors, W., Foo, Y., Hertkorn, N., Michel, C., & Stett-maier, K. (2001). Chemical studies of proanthocyanidins and hydrolysable tannins. Antioxid. Redox. Sign, 3, 995–1008. Boyd, B., Ford, C., Koepke, M., Gary, K., Horn, E., & et al. (2003). Etude pilote ouverte de l’effet antioxydant d’Ambrotose sur des personnes en bonne santé. Glycoscience & Nutrition, 4(6), 7.
5. Cheurfa, M., & Allem, R. (2016). Evaluation of antioxidant activity of different extracts of Aloysia triphylla leaves (L’Herit.) from Algeria in vitro. Phytotherapie, 14(3). https://doi.org/10.1007/s10298-015-0969-4
6. De Quirós, A. R.-B., Lage-Yusty, M. A., & López-Hernández, J. (2009). HPLC-analysis of polyphenolic compounds in Spanish white wines and determination of their antioxidant activity by radical scavenging assay. Food Research International, 42(8), 1018–1022. https://doi.org/10.1016/j.foodres.2009.04.009
7. El-Waziry, A. . (2007). Nutritive value assessment of ensiling or mixing Acacia and Atriplex using in vitro gas production technique Title. Res. J. Agric. Biol. Sci, 3(6), 605–614.
8. Fant, J., Grandon, B., Rathinasabapathi, B., Olson, S., & Lyrene, P. (2005). Potentiel antioxydant des fruits: l’évaluation de deux cultivars de bleuets à l’aide de test in vitro. Proc. Fla Etat Hort. Soc, 118, 250–252.

9. Favier, A. (2003). Le stress oxydant Intérêt conceptuel et expérimental dans la compréhension. Journal de La Société Chimique de France, (Novembre/Decembre), 108–115. https://doi.org/10.1016/j.cub.2014.07.028
10. Ferreria, A., Proenca, C., Serralheiro, L., & Aranjo, M. (2008). The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plant from Portugal. Journal of Ethnopharmacology, 108, 31–37.
11. Gormez, A. A., & Sengul, M. (2007). Total phenolics, mineral contents, antioxidant and antibacterial activities of Glycyrrhiza glabra L . roots grown wild in Turkey Volume. (December 2014).
12. Heim, K. E., Tagliaferro, A. R., & Bobilya, D. J. (2002). Flavonoid antioxidants: chemistry, metabolism, and structure-activity relationships. The Journal of Nutritional Biochemistry, 13(10), 572–584. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12550068
13. Kang, D. G., Yun, C. keun, & Lee, H. S. (2003). Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. Journal of Ethnopharmacology, 87(2–3), 231–236. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12860314
14. Ksouri, R., Megdiche, W., Falleh, H., Trabelsi, N., Boulaaba, M., Smaoui, A., & Abdelly, C. (2008). Influence of biological, environmental, and technical factors on phenolic content and antioxidant activities of Tunisian halophytes. Comptes Rendus Biologies, 331(11), 865–873. https://doi.org/10.1016/j.crvi.2008.07.024
15. Naz, S., Sultana, B., Shahid, M., & Ur-Rehman, K. (2013). Alteration in antioxidant and antimicrobial attributes of leaves of Zizyphus species in response to maturation. Journal of Medicinal Plants Research, 7(2), 61–70.
16. Sanchez-Moreno, C. (2002). Review: Methods Used to Evaluate the Free Radical Scavenging Activity in Foods and Biological Systems. Food Science and Technology International, 8(3), 121–137. https://doi.org/10.1106/108201302026770
17. Sroka, Z. (2005). Antioxidative and antiradical properties of plant phenolics. Zeitschrift Fur Naturforschung. C, Journal of Biosciences, 60(11–12), 833–843. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16402542
18. Suhayla, K. M. (2014). Antioxidative Activities of Aqueous and Ethanolic Extracts of Licorice Roots. Pakistan Journal of Nutrition, 13(5), 267–270. https://doi.org/10.3923/pjn.2014.267.270
19. Sultana, S., Haque, A., Hamid, K., Urmi, K., & Roy, S. (2010). Antimicrobial, cytotoxic, and antioxidant activity of methanolic extract of Glycyrrhiza glabra. Agriculture and Biology Journal of North America, 1(5), 957–960. https://doi.org/10.5251/abjna.2010.1.5.957.960
20. Sun, B., Ricardo-da-Silva, J., & Spranger, I. (1998). Critical Factors of Vanillin Assay for Catechins and Proanthocyanidins. J. Agric. Food Chem, 46(10), 4267–4274. https://doi.org/10.1021/JF980366J
21. Tepe, B., Daferera, D., Sökmen, M., Polissiou, M., & Sökmen, A. (2004). In Vitro Antimicrobial and Antioxidant Activities of the Essential Oils and Various Extracts of Thymus eigii M. Zohary et P.H. Davis. Journal of Agricultural and Food Chemistry, 52(5), 1132–1137. https://doi.org/10.1021/jf035094l
22. Walker, J. E., Saraste, M., Runswick, M. J., & Gay, N. J. (1982). Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. The EMBO Journal, 1(8), 945–951. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6329717
23. Wong, S., Leong, L., & Williamkoh, J. (2006). Antioxidant activities of aqueous extracts of selected plants. Food Chemistry, 99(4), 775–783. https://doi.org/10.1016/j.foodchem.2005.07.058
24. Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555–559. https://doi.org/10.1016/S0308-8146(98)00102-2
25. Zhou, X., Peng, J., Fan, G., & Wu, Y. (2005). Isolation and purification of flavonoid glycosides from Trollius ledebouri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase. Journal of Chromatography A, 1092(2), 216–221. https://doi.org/10.1016/j.chroma.2005.07.064