Identification, Genetic Diversity and Biological Control of Dollar Spot Disease Caused by Sclerotinia homoeocarpa on Golf Courses in Turkey

Year 2021, Volume: 5 Issue: 2, 147 - 153, 28.12.2021

Filiz Ünal Ayşe Aşkın Ercan Koca Mesut Yıldırır

Abstract

Dollar spot caused by Sclerotinia homoeocarpa, can create damage to warm and cool season turfgrasses, is a significant disease. In the study, 13 golf courses in 5 provinces of Turkey were surveyed for dollar spot disease caused by Sclerotinia homoeocarpa. During the survey samples were taken from leaves with cream spots, framed by a reddish brown edge and from areas of light yellow small patches. Identifications of Sclerotinia isolates were performed by DNA sequencing analysis. Five bacterial strains were examined to detect their antifungal influences against the dollar spot by using seed coating method in greenhouse conditions. As a result of isolations from infected plants from 13 golf courses, 7 Sclerotinia homoeocarpa (Sh) isolates were obtained. Pathogenicity tests performed in the greenhouse ranged from 81.03% to 90.75%. Consequense of biologic control studies, Pseudonomas putida 88cfp, Pseudomonas putida 166fp and Bacillus cereus 44bac were found efficient the ratio of 92.99%, 88.71% and 87.50% respectively. In further studies, effective bacterial strains (88cfp, 166fp and 44bac) should be carried out in large golf courses.

Keywords

Sclerotinia homoeocarpa, turfgrass, virulence, biological control

Supporting Institution

Plant Protection Central Research Institute

References

• Aşkın, A. (2008) The effects of non-pathogenic Pseudomonas on damping-off of tomato seedlings caused by some fungal pathogens in Ankara province. Phd. thesis. Ankara University Graduate School of Natural and Applied Sciences Department of Plant Protection.
• Aşkın, V., Coşkuntuna, A., Ünal, F. (2018) Çimlerdeki Fusarium graminearum ve Sclerotinia homoeocarpa F.T. Benn.'in Biyolojik Kontrolünün Araştırılması. Journal of Turkish Phytopathology, 47 ( 3), 31-40.
• Balcı, V., Gedikli, N. (2012) Golf alanlarında kullanılan kimyasal ilaçların ve gübrelerin çevre ve uygulayıcılar üzerine etkileri-organik yaklaşımlar. Spormetre Beden Eğitimi ve Spor Bilimleri Dergisi, IX (4), 141-148.
• Bennett, F.T. (1937) Dollar spot disease of turfgrass and its causal organism Sclerotinia homoeocarpa n sp. Ann Appl Biol 24: 236-257.
• Carbone, I., Kohn, L.M. (1993) Ribosomal DNA sequence divergence within internal transcribed spacer 1 of the Sclerotiniaceae. Mycologia 85: 415-427.
• Compant, S., Duffy, B., Nowak J., Clément C., Barka E.A. (2005) Use of Plant Growth-Promoting Bacteria for Biocontrol of Plant Diseases: Principles, Mechanisms of Action, and Future Prospects. Appl Environ Microbiol., 71(9): 4951–4959.
• Goodman, D.M., Burpee, L.L. (1991) Biological control of dollar spot disease of creeping bentgrass. Phytopathology 81: 1438-1446. Hodges, C.F., Campbell, D.A., Christians, N.E. (2007) Potential biocontrol of Sclerotinia homoeocarpa and Bipolaris sorokiniana on the phylloplane of Poa pratensis with strains of Pseudomonas spp. Plant Pathology 43(3): 500-506.
• Hsiang, T., Yang, L., Barton, W. (1998) Relative virulence of isolates of Sclerotinia homoeocarpa with varying sensitivity to propiconazole. Eur. Plant Pathol 104: 163-169.
• Holst-Jensen, A., Kohn, L.M., Schumacher, T. (1997) Nuclear rDNA phylogeny of Sclerotiniaceae. Mycologia 89: 885-899.
• Landschoot, P.J., McNitt, A.S. (1997) Effect of nitrogen fertilizers on suppression of dollar spot disease of Agrostis stolonifera L. Intl. Turf. Soc. Res. J. 8:905–911
• Liberti, D., Rollins, J.A., Harmon, P.F. (2012) Evidence for morphological, vegetative, genetic, and mating-type diversity in Sclerotinia homoeocarpa. Phytopathology 102: 506-518.
• Lahmyed, H., Bouharroud, R., Qessaoui, R., Ajerrar, A., Amarraque, A., Moulay, A.A., Chebli, B. (2021) Actinomycete as biocontrol agents against tomato gray mold disease caused by Botrytis cinerea, Kuwait J.Sci., 48 (3), 1-8.
• Liu, L.X., Hsiang, T., Carey, K., Eggens, J.L. (1995) Microbial populations and suppression of dollar spot disease in creeping bentgrass with inorganic and organic amendments. Plant Dis. 79:144–147.
• Mocioni, M., Gullino, M.L., Garibaldi, A. (2011) Sensitivity of Sclerotinia homoeocarpa isolates from turfgrass in Italy to demethylation-inhibiting (DMI) fungicides and iprodione. Phytopathologia Mediterranea 50, 408-413.
• Nelson, E., Craft, C.M. (1991a) Suppression of dollar spot with top-dressings amended with composts and organic fertilizers, Biol. Cult. Tests Control Plant Dis. 6:93.
• Nelson, E., Craft, C.M. (1991b) Introduction and establishment of strains of Enterobacter cloacae in golf course turf for the biological control of dollar spot. Plant Dis. 75:510–514.
• Powell, J.F., Vargas, J.M. (2000) Management of dollar spot on creeping bentgrass with metabolites of Pseudomonas aureofaciens (Tx-1). Plant Disease 84: 19-24.
• Rodriguez, F., Pfender, W.F. (1997) Antibiosis and antagonism of Sclerotinia homoeocarpa and Drechslera poae by Pseudomonas fluorescens Pf5 in vitro and in planta. Phytopathology 87: 614– 621.
• Salgado-Salazar, C., Lisa, A.B., Ismaiel, A., Boehm, M.J., Carbon, I., Putman, A.I., Tredway, L.P., Clarke, B.B., Crouch, J.A. (2018) Clarireedia: A new fungal genus comprising four pathogenic species responsible for dollar spot disease of turfgrass. Fungal Biology 122, 761-773.
• Schumann, G.L., Reuter, H.M. (1993) Suppression of dollar spot with wheat bran top-dressings, 1992. Biol. Cult. Tests Control Plant Dis. 8:113.
• Smiley, R., Dernoeden, P., Clarke, B. (1992) Compendium of turfgrass diseases, United States of America, 96pp.
• Townsend, G.K., Heuberger, J.W. (1943) Methods for estimating losses caused by diseases in fungicide experiments. Plant Dis. Reptr., 27, 340-343.
• Ünal, F., Aşkın, A., Koca, E., Yıldırır, M., Bingöl, M.Ü. (2019) Mycelial compatibility groups, pathogenic diversity and biological control of Sclerotium rolfsii on turfgrass. Egyptian Journal of Biological Pest Control 29: 44.
• Vargas, J.M. Jr., Powell, J.F. (1997) Mycelial compatibility and systematics of Sclerotinia homoeocarpa. Phytopathology 87: p.79. (Abstr.)
• Viji, G., Uddin, W., O’Neill, N.R., Mischke, S., Saunders, J.A. (2004) Genetic diversity of Sclerotinia homoeocarpa isolates from turfgrasses from various regions in North America. Plant Dis. 88: 1269-1276.
• Vincelli, P., Clarke, B., Munshaw, G. (2017) “Chemical control of turfgrass diseases 2017.” University of Kentucky, College of Agriculture, Food and Environment Cooperative Extension Service. 32p.
• Watschke, T.L., Dernoeden, P.H., Shetlar, D.J. (2013) Managing turfgrass pests, Second Edition. CRC Press, Taylor and Francis Group. 473pp.
• White, T.J., Bruns, T., Lee, S., Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. Innis, MA, Gelfand DH, Sninsky JJ, White TJ. Academic Press, Inc., New York, 315-322.
• Zhou, T., Boland, G.J. (1997) Hypovirulence and double-stranded RNA in Sclerotinia homoeocarpa. Phytopathology 87:147–153.
• Zhou, T., Boland, G.J. (1998) Suppression of dollar spot by hypovirulent isolates of Sclerotinia homoeocarpa. Phytopathology 88:788–794.