Antioxidant Enzymes Activities of Walnut Nursery Trees to Drought Stress Progression

Year 2021, Volume: 5 Issue: 2, 217 - 225, 28.12.2021

Fatma Yıldırım , Meltem Esen , Selçuk Binici , Civan Çelik , Adnan Yıldırım , Yaşar Karakurt

Abstract

This research was carried out to determine the biochemical responses of Chandler and Fernor walnut cultivars saplings grafted on the Juglans regia seedling rootstock under water stress in 2020. In accordance with this purpose; three different irrigation levels were applied to the one-year-old seedlings in polyethylene tubes in the greenhouse for three months: 1) full irrigation as control (100% of potted field capacity (PFC)) and 2) two different levels of restricted water application (50 and 25% of PFC). The amount of decreasing water was provided every 5 days. The antioxidant enzyme activities including ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in the leaves were determined every 15 days. In terms of the properties examined, statistically significant differences were found between the applications in the all analysis periods. In general, as the severity and duration of the water stress increased, the antioxidant enzyme activities gradually increased and while the highest values were determined in the 25% PFC application, the lowest values were determined in the 100% PFC control application.
As a result, the walnut saplings developed antioxidant defense mechanisms against water stress, demonstrating a possible tolerance. This suggests that the tolerance may be due to the activation of antioxidant systems and the reduction of oxidative damage. It has been determined that the antioxidant enzyme activities have different tendencies for both cultivars in response to the oxidative damage. While POD and CAT activities were at the higher levels in the Chandler cultivar; SOD and APX activities were at the higher levels in the Fernor cultivar.

Keywords

Juglans regia, Fernor, Chandler, Water stress, Enzyme

Supporting Institution

TÜBİTAK

Project Number

119O806

Thanks

This project was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK) with the number 119O806. We would like to thank TÜBİTAK. Also we would like to thank Prof. Dr. Yusuf UÇAR assiting in the calculation and application of irrigation water levels in the study.

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