Isolation of Cold Tolerant Bacteria from Antalya Region and Analysis of Their Growth Rates

Abstract

Only a portion of chemical fertilizers applied to soil can be used by plants, because nutrients can become insoluble in soil. Application of biofertilizers can make the nutrients bio-available for plants. This practice would be beneficial for both plant productivity and the environment. Aim of this study was to isolate and compare growth rates of bacterial strains isolated from low altitude and high altitude regions of Antalya. Our results showed that, bacterial strains isolated from high alitudes have higher growth rates at room temperature (15-25 C) and temperatures as low as 2 C, compared to strains isolated from low altitudes. This suggests that strains that can grow at 2 C also would have higher enzymatic activities at low temperatures which makes them better candidates for development of biofertilizers. Using cold adapted biofertilizer would have a positive effect on plant productivity in agricultural areas located in cold regions of Türkiye. Therefore, isolation of these organisms and testing of their biofertilizer potential is important for our agricultural applications.

Keywords

• Psychrophilic bacteria
• cold tolerant bacteria
• bacterial isolation
• biofertilizer

Antalya Bölgesinden Soğuğa Dayanıklı Bakteri İzolasyonu ve Büyüme Hızlarının Analizi

Öz

Toprağa uygulanan kimyasal gübrelerin ancak küçük bir kısmı bitkiler tarafından kullanılabilmektedir. Çünkü besin maddeleri toprakta alınamaz forma geçmektedir. Biyogübre uygulaması besin maddelerini bitkiler için alınabilir forma sokar. Bu uygulama hem bitki üretimi hem de çevre için yararlı olabilir. Bu çalışmanın amacı Antalya’nın yüksek ve düşük rakımlı iki bölgesinden bakteri izolasyonu yapmak ve izole edilen suşların büyüme hızlarını karşılaştırmak olmuştur. Yüksek rakımdan izole edilen suşların hem oda sıcaklığında (15-25 C) hem de 2 C’de düşük rakımdan izole edilen suşa göre daha hızlı ürediği görülmüştür. Bu da 2 C’de üreyebilen suşların düşük sıcaklıklarda da yüksek enzim aktivitesine sahip olabileceğini öne sürmektedir. Soğuğa dayanıklı biyogübre kullanımı ülkemizin soğuk bölgelerindeki zirai alanlarda bitki büyümesine pozitif etki gösterebilir. Bu tür soğuğa dayanıklı suşların izolasyonu ve biyogübre aktivitelerinin test edilmesi zirai uygulamalarımız için önem taşımaktadır.

Anahtar Kelimeler

• Psikrofilik bakteriler
• soğuğa dayaniklı bakteriler
• bakteri izolasyonu
• biyogübre

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