Effects of Clonostachys Species as Biocontrol Agents Against Verticillium dahliae on Strawberry

Abstract

This study was conducted to test Clonostachys isolates in the biological control of wilt diseases caused by Verticillium dahliae on strawberry plants. As a result of the isolations made from strawberry plants, 32 Clonostachys isolates were obtained. Cultural, morphological and molecular characterization were used for the identification of these isolates. The isolates were identified as Clonostachys rosea f. rosea (18 isolates) and Clonostachys rosea f. catenulata (14 isolates). It was determined that the inhibition rates were not make a significant difference between the two species in the comparison of the pathogen with C. rosea f. rosea and C. rosea f. catenulata isolates, and the isolates of biocontrol agents had different percentage of effects in inhibiting the mycelial growth of the pathogen in in vitro condition. When the inhibition rates obtained as a result of the study were evaluated, it was determined that the most effective isolate was C. rosea f. rosea Gr13 (48.95%) and C. rosea f. rosea Gr4 (44.75%), respectively.

Keywords

• Verticillium dahliae
• Clonostachys rosea f. rosea
• Clonostachys rosea f. catenulata
• biological control

Çilekte Verticillium dahliae’ya Karşı Biyoetmen Olarak Clonostachys Türlerinin Etkileri

Öz

Çilek bitkilerinde solgunluk hastalığına neden olan Verticillium dahliae’nın biyolojik mücadelesinde Clonostachys izolatlarının test edilmesi bu çalışmanın amacını oluşturmuştur. Çalışma kapsamında çilek bitkilerinden yapılan izolasyonlar neticesinde 32 adet Clonostachys izolatı elde edilmiştir. Kültürel, morfolojik ve moleküler olarak tanıları yapılan bu izolatların Clonostachys rosea f. rosea (18 izolat) ve Clonostachys rosea f. catenulata (14 izolat) alt türlerine ait olduğu belirlenmiştir. In vitro koşullarda patojen ile C. rosea f. rosea ve C. rosea f. catenulata izolatları arasında yapılan karşılaştırma sonucunda engelleme oranlarının iki tür arasında önemli bir fark oluşturmadığı ve biyoetmenlere ait izolatların patojenin misel gelişimini engellemede farklı oranlarda etkiye sahip olduğu tespit edilmiştir. Çalışma sonucunda elde edilen engelleme oranları değerlendirildiğinde en etkili izolatın %48.95 oranı ile C. rosea f. rosea Gr13 olduğu, bu izolatı %44.75 oranı ile C. rosea f. rosea Gr4’ün takip ettiği belirlenmiştir.

Anahtar Kelimeler

• Verticillium dahliae
• Clonostachys rosea f. rosea
• Clonostachys rosea f. catenulata
• biyolojik mücadele

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