Siklamende Gama Işını Uygulamalarının Polen Davranışı Üzerine Etkisinin Değerlendirilmesi

Abstract

Bu çalışmanın amacı önemli bir saksılı süs bitkisi olan Cyclamen persicum L.'nin polenleri üzerine gama ışınlamasının etkileri değerlendirilmiştir. Polenler Co-60 kaynağı kullanılarak farklı dozlarda (0, 50, 100, 150, 200, 300, 450 Gy) gama ışını ile ışınlanmış ve polen canlılığı, polen çimlenme oranı ve in vitro’da çimlenen polenlerin tüp uzunluğu ölçülmüştür. Ek olarak, anilin mavisi floresan yöntemi uygulanarak in vivo polen gelişimi gözlenmiştir. Ortalama polen canlılığı %82.02 ile %87.03 arasında değişmiştir. En yüksek polen çimlenme oranı ışınlamadan 24 saat sonra kontrol grubunda (%66.13), en düşük oran ise ışınlamadan 72 saat sonra 450 Gy uygulamada (%28.51) belirlenmiştir. En kısa polen tüpü uzunluğu (48.02 µm) 72 saatlik ışınlanmamış polenlerde gözlenirken, en uzun polen tüpü (79.37 µm) ışınlamadan 24 saat sonra 300 Gy ile ışınlanmış polenlerde belirlenmiştir. Işınlama dozu 150 Gy'den 450 Gy'ye yükseldiğinde, polen çimlenmesi üzerinde ve stil içerisinde polen tüp uzamasında durağanlık gözlemlenmiştir.

Keywords

• Işınlama
• Polen tüpü uzaması
• Çimlenme
• Canlılık
• Letal doz

Project Number

118O728

Assessment of The Effect of Gamma Ray Treatments on Pollen Behavior in Cyclamen

Abstract

The aim of the study is to evaluate the effects of gamma irradiation on pollen grains of Cyclamen persicum L. which is a valuable ornamental pot plant. Pollens were irradiated at different doses (0, 50, 100, 150, 200, 300, 450 Gy) of gamma-ray using Co-60 source and pollen viability and in vitro pollen germination test were carried out and tube length of in vitro germinated pollens was measured. Additionally, in vivo pollen development was visualized by performing an aniline blue fluorescence method. Average pollen viability was ranged from 82.02% to 87.03%. The highest pollen germination rate (66.13%) was observed in the control group at 24 h after irradition, while the lowest rate (28.51%) was determined in 450 Gy treatments at 72 h after irradition. The shortest pollen tube length (48.02 µm) was observed at 72 h old non-irradiated pollen grains, while the most extended pollen tube length (79.37 µm) was determined in pollen grains irradiated with 300 Gy at 24 HAI. When the irradiation dose increases from 150 Gy to 450 Gy, inhibition on pollen germination and pollen tube elongation within the style were observed.

Keywords

• Irradiation
• Pollen tube elongation
• Germination
• Viability
• Lethal dose

Supporting Institution

TÜBİTAK

Project Number

118O728

Thanks

This study supported by The Scientific and Technological Research Council of Turkey (TUBITAK; project number 118O728). We thank for the technical support during gamma irradiation to Dr. Yaprak Taner Kantoğlu (Turkish Energy, Nuclear and Mineral Research Agency) and Prof. Dr. Sinan Eti and Dr. Şenay Karabıyık for technical support during histological analysis.

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