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Effects of Postharvest Chitosan and Salicylic Acid Treatments on Storage Duration and Quality of Minimally Processed ‘Hicaznar’ Pomegranate Varieties

Year 2025, Volume: 11 Issue: 2, 178 - 187, 29.08.2025
https://doi.org/10.24180/ijaws.1708247

Abstract

In recent years, pomegranate cultivation has significantly increased both in Turkey and worldwide. Consequently, the importance of postharvest preservation has grown in parallel with the rising volume of harvested pomegranates. This study aimed to investigate the effects of chitosan (Ch), salicylic acid (SA), and their combined application chitosan+salicylic acid (Ch+SA) on the quality attributes of pomegranate arils during cold storage. Following aril extraction, treatments were applied, and the arils were stored at 4 °C and 90% relative humidity for 16 days. At 4 day intervals during the storage period, samples were analyzed for weight loss, aril color (hue angle), total soluble solids (TSS), titratable acidity (TA), total phenolic content (TPC), total antioxidant activity (TAA), ascorbic acid (vitamin C) content (AsA), and total anthocyanin content (TA). Results indicated that both Ch and SA treatments were effective in maintaining the quality attributes of the arils compared to the control, with the combined application (Ch+SA) showing enhanced effectiveness. The Ch+SA treatment was particularly effective in reducing weight loss and slowing changes in hue angle, TSS, and TA. Additionally, it has been determined that postharvest application of chitosan+salicylic acid could be an effectice method for keeping quality attributes in pomegranate arils during a cold storage period of 16 days.

References

  • Amiri, A., Ramezanian, A., Mortazavi, S. M. H., Hosseini, S. M. H., & Yahia, E. (2021). Shelf‐life extension of pomegranate arils using chitosan nanoparticles loaded with Satureja hortensis essential oil. Journal of the Science of Food and Agriculture, 101(9), 3778-3786. https://doi.org/10.1002/jsfa.11010
  • Anean, H. A., Mallasiy, L. O., Bader, D. M., & Shaat, H. A. (2023). Nano edible coatings and films combined with zinc oxide and pomegranate peel active phenol compounds has been to extend the shelf life of minimally processed pomegranates. Materials, 16(4), 1569. https://doi.org/10.3390/ma16041569
  • Anonim, (2024). Bitkisel Üretim İstatistikleri. Türkiye İstatistik Kurumu (TÜİK), http://www.tuik.gov.tr. [Erişim tarihi: 03.03.2025].
  • Asghari, M., & Aghdam, M. S. (2010). Impact of salicylic acid on post-harvest physiology of horticultural crops. Trends in Food Science & Technology, 21(10), 502-509. https://doi.org/10.1016/j.tifs.2010.07.009
  • Ayhan, Z., & Eştürk, O. (2009). Overall quality and shelf life of minimally processed and modified atmosphere packaged “Ready-to-Eat” pomegranate arils. Journal of Food Science, 74(5), 399-405. https://doi.org/10.1111/j.1750-3841.2009.01184.x
  • Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1996.0292
  • Bhatia, K., & Asrey, R. (2018). Effect of salicylic acid pre-treatment on functional and sensory quality of minimally processed pomegranate (Punica granatum L) arils. Journal of Scientific and Industrial Research, 77, 125-130. http://nopr.niscpr.res.in/handle/123456789/43538
  • Can Çetin, A. (2012). Effects of edible chitosan coating on quality parameters of pomegranate (Punica granatum) arils [Yüksek Lisans Tezi, Orta Doğu Teknik Üniversitesi]. https://tez.yok.gov.tr/UlusalTezMerkezi/.
  • Caro, Y., & Joas, J. (2005). Postharvest control of litchi pericarp browning (cv. Kwai Mi) by combined treatments of chitosan and organic acids: II. Effect of the initial water content of pericarp. Postharvest Biology and Technology, 38(2), 137–144. https://doi.org/10.1016/j.postharvbio.2005.06.012
  • Cemeroğlu, B. (2007). Gıda analizleri. Gıda Teknolojisi Derneği Yayınları, 34, 168-171.
  • Cheng, G .W., & Breen, P. J. (1991). Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. Journal of the American Society for Horticultural Science, 116(5), 865-869. https://doi.org/10.21273/jashs.116.5.865
  • Çandır, E., Özdemir, A.E., Göv, T., Kanak, A., & Özçelik, H. (2020). Minimum işlenmiş ‘Katırbaşı’ nar çeşidinin soğukta muhafazası. Alatarım Dergisi 19 (1) 9‒15.
  • Erkan, M., & Doğan, A. (2018). Pomegranate/Roma—Punica granatum. In : Exotic Fruits (Ed:Rodrigues, S., Silva, E.O., Brito, E.S.) In, Eds, Elsevier, United Kingtom, p. 355-361. https://doi.org/10.1016/B978-0-12-803138-4.00049-6
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  • Ghasemnezhad, M., Zareh, S., Rassa, M., & Sajedi, R. H. (2013). Effect of chitosan coating on maintenance of aril quality: microbial population and PPO activity of pomegranate (Punica granatum L. cv. Tarom) at cold storage temperature. Journal Science Food Agriculture, 93, 368–374. https://doi.org/10.1002/jsfa.5770.
  • Gil, I., Toma, F. A., Hess-pierce, B., Holcroft, D. M., & Kader, A. A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48(10), 4581–4589. https://doi.org/10.1021/jf000404a.
  • Gil, M. I., Artés, F., & Tomas‐Barberan, F. A. (1996). Minimal processing and modified atmosphere packaging effects on pigmentation of pomegranate seeds. Journal of Food Science, 61(1), 161-164. https://doi.org/10.1111/j.1365- 621.1996.tb14749.x
  • Giménez, M. J., Serrano, M., Valverde, J. M., Martínez‐Romero, D., Castillo, S., Valero, D., & Guillen, F. (2017). Preharvest salicylic acid and acetylsalicylic acid treatments preserve quality and enhance antioxidant systems during postharvest storage of sweet cherry cultivars. Journal of the Science of Food and Agriculture, 97(4), 1220-1228. https://doi.org/10.1002/jsfa.7853
  • Holland, D., Hatib, K., & Bar-Ya’akov, I. (2009). Pomegranate botany, horticulture breeding. Horticultural Reviews. 35: 127–191.
  • Jiao, W., Shu, C., Li, X., Cao, J., Fan, X., & Jiang, W. (2019). Preparation of a chitosan-chlorogenic acid conjugate and its application as edible coating in postharvest preservation of peach fruit. Postharvest Biology and Technology, 154, 129–136. https://doi.org/10.1016/j.postharvbio.2019.05.003
  • Kumar, A., & Deb, P. (2019). Effect of edible coating on fresh pomegranate arils. Journal of Postharvest Technology, 7(1), 25-32.
  • Mahdi, E. M. M. (2020). Danelenmiş ‘Hicaznar’ (Punica granatum) çeşidinde farklı hasat sonrası uygulamaların kalite özellikleri üzerine etkileri [Yüksek Lisans Tezi, Selçuk Üniversitesi]. https://tez.yok.gov.tr/UlusalTezMerkezi/.
  • Martínez-Romero, D., Castillo, S., Guillén, F., Díaz-Mula, H. M., Zapata, P. J., Valero, D., & Serrano, M. (2013). Aloe vera gel coating maintains quality and safety of ready-to-eat pomegranate arils. Postharvest Biology Technolocy, 86, 107–112. https://doi.org/10.1016/j.postharvbio.2013.06.022
  • McGuire, R. G. (1992). Reporting of objective color measurements. HortScience, 27(12), 1254-1255.
  • Mena, P., Garcia-Viguera, C., Navarro-Rico, J., Moreno, D. A., Bartual, J., Saura, D., & Marti, N. (2011). Phytochemical characterisation for industrial use of pomegranate (Punica granatum L.) cultivars grown in Spain. Journal of the Science of Food Agriculture, 91(10), 1893–1906. https://doi.org/10.1002/jsfa.4411
  • Mirdehghan, S. H., Rahemi, M., Serrano, M., Guillén, F., Martínez-Romero, D., & Valero, D. (2006). Prestorage heat treatment to maintain nutritive and functional properties during postharvest cold storage of pomegranate. Journal of Agricultural and Food Chemistry, 54(22), 8495–8500. https://doi.org/10.1021/jf0615146
  • Munhuweyi K., Cheryl L., Lennox, C. L., Meitz-Hopkins, J. C., Calebc, O. J., Sigge, G. O., & Umezuruike Linus Oparaa, U. L. (2017). Investigating the effects of crab shell chitosan on fungal mycelial growth and postharvest quality attributes of pomegranate whole fruit and arils. Scientia Horticulturae 220, 78–89. https://doi.org/10.1016/j.scienta.2017.03.038
  • Mwelase, S., Opara, U. L., & Fawole, O. A. (2022). Effect of chitosan‐based melatonin composite coating on the quality of minimally processed pomegranate aril‐sacs during cold storage. Journal of Food Processing and Preservation, 46(12), e17096. https://doi.org/10.1111/jfpp.17096
  • Onursal, C. E., Güneyli, A., Seçmen, T., Eren, İ., Koyuncu, M. A., & Erbaş, D. (2016). Hasat sonrası salisilik asit uygulamasının Dr. Jules Guyot armut çeşidinde muhafaza ve raf ömrü kalitesi üzerine etkileri. Bahçe Özel Sayı: VII Ulusal Bahçe Bitkileri Kongresi Bildirileri- Cilt I: Meyvecilik: 188-193.
  • Opara, U. L., Hussein, Z., & Caleb, O. J. (2015). Phytochemical properties and antioxidant activities of minimally processed “Acco” pomegranate arils as affected by perforation‐mediated modified atmosphere packaging. Journal of Food Processing and Preservation, 41(3), e12948. https://doi.org/10.1111/jfpp.12948
  • Özdemir, K., & Gökmen, V. (2017). Extending the shelf-life of pomegranate arils with chitosan-ascorbic acid coating. LWT Food Science and Technology, 76, 172-180. https://doi.org/10.1016/j.lwt.2016.10.057
  • Pearson, D., Surry, J., & Chuchill, A. (1970). The chemical analysis of food national college of food technology. University Of Reading, Weybridge Surry T and A Churchill.
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Hasat Sonrası Kitosan ve Salisilik Asit Uygulamalarının Danelenmiş ‘Hicaznar’ Çeşidinde Muhafaza Süresi ve Kalite Üzerine Etkileri

Year 2025, Volume: 11 Issue: 2, 178 - 187, 29.08.2025
https://doi.org/10.24180/ijaws.1708247

Abstract

Nar yetiştiriciliği ülkemizde ve dünyada son yıllarda çok hızlı bir şekilde artmaktadır. Hasat edilen nar miktarındaki artışa bağlı olarak muhafazası da aynı oranda önem kazanmıştır. Bu çalışmada danelenmiş narlara kitosan, salisilik asit ve bu uygulamaların birlikte kullanımı olan kitosan+salisilik asitin hasat sonrası soğukta muhafaza süresince kalite özellikleri üzerine etkileri araştırılmıştır. Daneleme işlemi yapılan narlar uygulamalar yapıldıktan sonra 4 °C ve %90 oransal nem içeren soğuk hava deposunda 16 gün süreyle depolanmıştır. Muhafaza süresince 4 gün arayla danelerde ağırlık kaybı, dane rengi (hue açısı), suda çözünebilir toplam kuru madde miktarı (SÇKM), titre edilebilir asitlik miktarı (TEA), toplam fenolik madde miktarı (TFM), toplam antioksidan aktivite miktarı (TAA), askorbik asit (C vitamini) miktarı (AsA) ve toplam antosiyanin miktarı (TA) analiz edilmiştir. Araştırma sonucunda kitosan (Ch) ve salisilik asit (SA) uygulamasının kontrol ile karşılaştırıldığında danelerde kalite özelliklerini korumada etkili olduğu, bu etkinin birlikte kullanıldığında (Ch+SA) daha da arttığı saptanmıştır. Ch+SA uygulamasının ağırlık kaybının azaltılması, dane rengi, SÇKM, TEA değerlerindeki değişimin yavaşlatılmasında etkili bir uygulama olduğu görülmüştür. Ayrıca TAA, TFM, AsA ve TA kapsamlarında oluşan değişimlerin yavaşlatılmasında etkili olduğu tespit edilmiştir. Sonuç olarak 16 gün muhafaza edilen nar danelerinde hasat sonrası Ch ve SA uygulamalarının birlikte kullanımının kalite özelliklerini korumada etkili olduğu belirlenmiştir.

References

  • Amiri, A., Ramezanian, A., Mortazavi, S. M. H., Hosseini, S. M. H., & Yahia, E. (2021). Shelf‐life extension of pomegranate arils using chitosan nanoparticles loaded with Satureja hortensis essential oil. Journal of the Science of Food and Agriculture, 101(9), 3778-3786. https://doi.org/10.1002/jsfa.11010
  • Anean, H. A., Mallasiy, L. O., Bader, D. M., & Shaat, H. A. (2023). Nano edible coatings and films combined with zinc oxide and pomegranate peel active phenol compounds has been to extend the shelf life of minimally processed pomegranates. Materials, 16(4), 1569. https://doi.org/10.3390/ma16041569
  • Anonim, (2024). Bitkisel Üretim İstatistikleri. Türkiye İstatistik Kurumu (TÜİK), http://www.tuik.gov.tr. [Erişim tarihi: 03.03.2025].
  • Asghari, M., & Aghdam, M. S. (2010). Impact of salicylic acid on post-harvest physiology of horticultural crops. Trends in Food Science & Technology, 21(10), 502-509. https://doi.org/10.1016/j.tifs.2010.07.009
  • Ayhan, Z., & Eştürk, O. (2009). Overall quality and shelf life of minimally processed and modified atmosphere packaged “Ready-to-Eat” pomegranate arils. Journal of Food Science, 74(5), 399-405. https://doi.org/10.1111/j.1750-3841.2009.01184.x
  • Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1996.0292
  • Bhatia, K., & Asrey, R. (2018). Effect of salicylic acid pre-treatment on functional and sensory quality of minimally processed pomegranate (Punica granatum L) arils. Journal of Scientific and Industrial Research, 77, 125-130. http://nopr.niscpr.res.in/handle/123456789/43538
  • Can Çetin, A. (2012). Effects of edible chitosan coating on quality parameters of pomegranate (Punica granatum) arils [Yüksek Lisans Tezi, Orta Doğu Teknik Üniversitesi]. https://tez.yok.gov.tr/UlusalTezMerkezi/.
  • Caro, Y., & Joas, J. (2005). Postharvest control of litchi pericarp browning (cv. Kwai Mi) by combined treatments of chitosan and organic acids: II. Effect of the initial water content of pericarp. Postharvest Biology and Technology, 38(2), 137–144. https://doi.org/10.1016/j.postharvbio.2005.06.012
  • Cemeroğlu, B. (2007). Gıda analizleri. Gıda Teknolojisi Derneği Yayınları, 34, 168-171.
  • Cheng, G .W., & Breen, P. J. (1991). Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. Journal of the American Society for Horticultural Science, 116(5), 865-869. https://doi.org/10.21273/jashs.116.5.865
  • Çandır, E., Özdemir, A.E., Göv, T., Kanak, A., & Özçelik, H. (2020). Minimum işlenmiş ‘Katırbaşı’ nar çeşidinin soğukta muhafazası. Alatarım Dergisi 19 (1) 9‒15.
  • Erkan, M., & Doğan, A. (2018). Pomegranate/Roma—Punica granatum. In : Exotic Fruits (Ed:Rodrigues, S., Silva, E.O., Brito, E.S.) In, Eds, Elsevier, United Kingtom, p. 355-361. https://doi.org/10.1016/B978-0-12-803138-4.00049-6
  • Garcia, E., & Barrett, D. (2002). Preservative treatments for fresh-cut fruits and vegetables. In O. Lamiranka (Ed.), Fresh-cut Fruits and Vegetables: Science, Technology, and Market. Boca Raton: CRC Press.
  • Ghasemnezhad, M., Zareh, S., Rassa, M., & Sajedi, R. H. (2013). Effect of chitosan coating on maintenance of aril quality: microbial population and PPO activity of pomegranate (Punica granatum L. cv. Tarom) at cold storage temperature. Journal Science Food Agriculture, 93, 368–374. https://doi.org/10.1002/jsfa.5770.
  • Gil, I., Toma, F. A., Hess-pierce, B., Holcroft, D. M., & Kader, A. A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48(10), 4581–4589. https://doi.org/10.1021/jf000404a.
  • Gil, M. I., Artés, F., & Tomas‐Barberan, F. A. (1996). Minimal processing and modified atmosphere packaging effects on pigmentation of pomegranate seeds. Journal of Food Science, 61(1), 161-164. https://doi.org/10.1111/j.1365- 621.1996.tb14749.x
  • Giménez, M. J., Serrano, M., Valverde, J. M., Martínez‐Romero, D., Castillo, S., Valero, D., & Guillen, F. (2017). Preharvest salicylic acid and acetylsalicylic acid treatments preserve quality and enhance antioxidant systems during postharvest storage of sweet cherry cultivars. Journal of the Science of Food and Agriculture, 97(4), 1220-1228. https://doi.org/10.1002/jsfa.7853
  • Holland, D., Hatib, K., & Bar-Ya’akov, I. (2009). Pomegranate botany, horticulture breeding. Horticultural Reviews. 35: 127–191.
  • Jiao, W., Shu, C., Li, X., Cao, J., Fan, X., & Jiang, W. (2019). Preparation of a chitosan-chlorogenic acid conjugate and its application as edible coating in postharvest preservation of peach fruit. Postharvest Biology and Technology, 154, 129–136. https://doi.org/10.1016/j.postharvbio.2019.05.003
  • Kumar, A., & Deb, P. (2019). Effect of edible coating on fresh pomegranate arils. Journal of Postharvest Technology, 7(1), 25-32.
  • Mahdi, E. M. M. (2020). Danelenmiş ‘Hicaznar’ (Punica granatum) çeşidinde farklı hasat sonrası uygulamaların kalite özellikleri üzerine etkileri [Yüksek Lisans Tezi, Selçuk Üniversitesi]. https://tez.yok.gov.tr/UlusalTezMerkezi/.
  • Martínez-Romero, D., Castillo, S., Guillén, F., Díaz-Mula, H. M., Zapata, P. J., Valero, D., & Serrano, M. (2013). Aloe vera gel coating maintains quality and safety of ready-to-eat pomegranate arils. Postharvest Biology Technolocy, 86, 107–112. https://doi.org/10.1016/j.postharvbio.2013.06.022
  • McGuire, R. G. (1992). Reporting of objective color measurements. HortScience, 27(12), 1254-1255.
  • Mena, P., Garcia-Viguera, C., Navarro-Rico, J., Moreno, D. A., Bartual, J., Saura, D., & Marti, N. (2011). Phytochemical characterisation for industrial use of pomegranate (Punica granatum L.) cultivars grown in Spain. Journal of the Science of Food Agriculture, 91(10), 1893–1906. https://doi.org/10.1002/jsfa.4411
  • Mirdehghan, S. H., Rahemi, M., Serrano, M., Guillén, F., Martínez-Romero, D., & Valero, D. (2006). Prestorage heat treatment to maintain nutritive and functional properties during postharvest cold storage of pomegranate. Journal of Agricultural and Food Chemistry, 54(22), 8495–8500. https://doi.org/10.1021/jf0615146
  • Munhuweyi K., Cheryl L., Lennox, C. L., Meitz-Hopkins, J. C., Calebc, O. J., Sigge, G. O., & Umezuruike Linus Oparaa, U. L. (2017). Investigating the effects of crab shell chitosan on fungal mycelial growth and postharvest quality attributes of pomegranate whole fruit and arils. Scientia Horticulturae 220, 78–89. https://doi.org/10.1016/j.scienta.2017.03.038
  • Mwelase, S., Opara, U. L., & Fawole, O. A. (2022). Effect of chitosan‐based melatonin composite coating on the quality of minimally processed pomegranate aril‐sacs during cold storage. Journal of Food Processing and Preservation, 46(12), e17096. https://doi.org/10.1111/jfpp.17096
  • Onursal, C. E., Güneyli, A., Seçmen, T., Eren, İ., Koyuncu, M. A., & Erbaş, D. (2016). Hasat sonrası salisilik asit uygulamasının Dr. Jules Guyot armut çeşidinde muhafaza ve raf ömrü kalitesi üzerine etkileri. Bahçe Özel Sayı: VII Ulusal Bahçe Bitkileri Kongresi Bildirileri- Cilt I: Meyvecilik: 188-193.
  • Opara, U. L., Hussein, Z., & Caleb, O. J. (2015). Phytochemical properties and antioxidant activities of minimally processed “Acco” pomegranate arils as affected by perforation‐mediated modified atmosphere packaging. Journal of Food Processing and Preservation, 41(3), e12948. https://doi.org/10.1111/jfpp.12948
  • Özdemir, K., & Gökmen, V. (2017). Extending the shelf-life of pomegranate arils with chitosan-ascorbic acid coating. LWT Food Science and Technology, 76, 172-180. https://doi.org/10.1016/j.lwt.2016.10.057
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There are 45 citations in total.

Details

Primary Language Turkish
Subjects Post Harvest Horticultural Technologies (Incl. Transportation and Storage)
Journal Section Bahçe Bitkileri
Authors

Sevil Ünal 0000-0002-7399-4523

Early Pub Date August 28, 2025
Publication Date August 29, 2025
Submission Date May 28, 2025
Acceptance Date July 9, 2025
Published in Issue Year 2025 Volume: 11 Issue: 2

Cite

APA Ünal, S. (2025). Hasat Sonrası Kitosan ve Salisilik Asit Uygulamalarının Danelenmiş ‘Hicaznar’ Çeşidinde Muhafaza Süresi ve Kalite Üzerine Etkileri. Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 11(2), 178-187. https://doi.org/10.24180/ijaws.1708247

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