Determination of the Genetic Variation within the Egg Parasitoid, Trichogramma brassicae, Bezdenko (Hymenoptera: Trichogrammatidae) Populations in Düzce Province, Turkey

Abstract

Members of Trichogramma genus are the most widely used as natural enemies in biological control programs. Choice of natural enemies is known to have a critical important for the succession of biological control. Their morphologic identification has some bottlenecks as laborious, time-consuming and mostly based on very small structures of the bodies. Molecular techniques have been widely used for detection, discrimination, phylogenetic analysis, identification and characterization in biological control agents belong to Trichogramma spp. In this study, the efficacy of the direct sequencing analysis of internal transcribed spacer locus of rDNA was investigated for analyzing the genetic variation within nine Trichogramma brassicae population from corn growing areas in Düzce province. The comparison of the sequences was performed with other ITS2 locus of Trichogramma species available in GenBank database. The result of analyses indicated that no genetic variation was determined in the Düzce population.

Keywords

• Trichogramma brassicae
• egg parasitoid
• genetic variation
• Düzce

Yumurta Parazitoiti, Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae)’nin Düzce İlindeki Popülasyonları İçerisindeki Genetik Varyasyonların Belirlenmesi

Öz

Trichogramma türleri, biyolojik mücadele programlarında en çok kullanılan doğal düşmanlardandır. Doğal düşmanın seçiminin, biyolojik kontrolün başarısı açısından kritik bir öneme sahip olduğu bilinmektedir. Bu cinse bağlı türlerin morfolojik teşhisleri zahmetli olmaları, zaman kaybı oluşturmaları ve vücut yapılarının oldukça küçük boyutlarda olmasına bağlı olarak problem oluşturmaktadır. Moleküler teknikler, Trichogramma cinsine ait biyolojik kontrol ajanlarında tespit etme, ayırt etme, filogenetik analiz, tanımlama ve karakterizasyon için yaygın olarak kullanılmaktadır. Bu çalışmada, Düzce ilinde mısır yetiştirilen alanlardan elde edilen dokuz Trichogramma brassicae popülasyonunun genetik varyasyon analizi için rDNA’nın ITS bölgesinin
doğrudan sekanslama analizinin etkinliği araştırılmıştır. Sekansların karşılaştırılması, GenBank veri tabanında mevcut olan Trichogramma türlerinin ITS2 bölgeleri ile gerçekleştirilmiştir. Analiz sonuçları, Düzce popülasyonlarında genetik varyasyonun tespit edilmediğini göstermiştir.

Anahtar Kelimeler

• Trichogramma brassicae
• yumurta parazitoiti
• genetic varyasyon
• Düzce

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