Rhizoctonia Ağ Yanıklığı Hastalığına Karşı Bazı Fasulye Çeşitlerinin Reaksiyonları

Öz

Rhizoctonia solani fasulye üretim alanlarında yaygın olarak görülen ve ağ yanıklığı, kök ve hipokotil çürüklüğüne sebep olarak önemli ekonomik kayıplar oluşturan bir hastalık etmenidir. Bu çalışma kapsamında Rhizoctonia ağ yanıklığı hastalığına karşı ülkemizde yaygın olarak yetiştirilen 30 fasulye çeşidinin hastalık reaksiyonları yaprak inokulasyon yöntemi ile değerlendirilmiştir. Patojen izolatı PCR-RFLP yöntemi ile R. solani AG-4 HG II olarak sınıflandırılmıştır. Çalışma kapsamında değerlendirilen fasulye çeşitlerinin hiçbiri hastalığa tam dayanıklı değil iken 13 çeşit orta dayanıklı, 17 çeşit ise hassas olarak değerlendirilmiştir. Test edilen çeşitler arasında en dayanıklı çeşit 40 günlük fasulye çeşidi olup bunu Java ve Klas fasulye çeşitleri takip etmiştir.

Anahtar Kelimeler

• Ağ yanıklığı,fasulye,hastalık reaksiyonu,Rhizoctonia solani.

Reactions of Some Common Bean Cultivars to Rhizoctonia Web Blight

Abstract

Rhizoctonia solani causing web blight, root and hypocotyl rot of bean is a prevalent and economically important pathogen in bean production areas. The aim of study was to evaluate the disease reactions of 30 common bean cultivars that commonly grown in Turkey against Rhizoctonia web blight using leaf inoculation method. Pathogen isolate was classified as R. solani AG-4 HG II by PCR-RFLP method. None of the common bean cultivars evaluated in this study was completely resistant to the disease, while 13 cultivars were considered as moderately resistant and 17 cultivars as susceptible. Among the tested cultivars, the most resistant cultivar was the bean cultivar 40 günlük, followed by the common bean cultivars Java and Klas.

Keywords

• Web blight,bean,Disease reaction,Rhizoctonia solani.

References

1. Akarca, Z. (2013). Erzincan ilinde fasulye bitkilerinin toprak üstü aksamlarından izole edilen Rhizoctonia türlerinin anastomosis grupları ve patojenitesi. Yüksek Lisans Tezi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Erzurum.
2. Bautista-Pérez, M. D. J., & Echávez-Badel, R. (2000). Methodology for screening common bean resistance to web blight. The Journal of Agriculture of the University of Puerto Rico, 84(1-2), 91-94.
3. Beaver, J. S., Zapata, M., Alameda, M., Porch, T. G., & Rosas, J. C. (2012). Registration of PR0401-259 and PR0650-31 dry bean germplasm lines. Journal of Plant Registrations, 6(1), 81-84.
4. Demirci, E., & Doken, M. T. (1995). Anastomosis groups of Rhizoctonia solani Kuhn and binucleate Rhizoctonia isolates from various crops in Turkiye. Journal of Turkish Phytopathology, 24(2), 57-62.
5. Eken, C., & Demirci, E. (2004). Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia isolates from bean in Erzurum, Turkey. Journal of Plant Pathology, 49-52.
6. Erper, I., Ozkoc, I., & Karaca, G. H. (2011). Identification and pathogenicity of Rhizoctonia species isolated from bean and soybean plants in Samsun, Turkey. Archives of Phytopathology and Plant Protection, 44(1), 78-84.
7. Erper, İ., Karaca, G. H., & Balkaya, A. (2003). Samsun ilinde üretimi yapılan bazı taze fasulye çeşitlerinin Rhizoctonia solani'ye duyarlılıklarının belirlenmesi. OMÜ Ziraat Fakültesi Dergisi, 18, 3-6.
8. FAOSTAT. (2018). Gıda ve tarım örgütü. http://www.fao.org/faostat/en/#data/QC. Erişim tarihi: 15 Mayıs 2019.

9. Galindo, J. J., Abawi, G. S., & Thurston, H. D. (1982). Variability among isolates of Rhizoctonia solani associated with snap bean hypocotyls and soils in New York. Plant Disease, 66(5), 390-394.
10. Godoy-Lutz, G., Kuninaga, S., Steadman, J. R., & Powers, K. (2008). Phylogenetic analysis of Rhizoctonia solani subgroups associated with web blight symptoms on common bean based on ITS-5.8 S rDNA. Journal of General Plant Pathology, 74(1), 32-40.
11. Godoy-Lutz, G., Steadman, J. R., Higgins, B., & Powers, K. (2003). Genetic variation among isolates of the web blight pathogen of common bean based on PCR-RFLP of the ITS-rDNA region. Plant Disease, 87(7), 766-771.
12. González, N., Godoy-Lutz, G., Steadman, J. R., Higgins, R., & Eskridge, K. M. (2012). Assessing genetic diversity in the web blight pathogen Thanatephorus cucumeris (anamorph= Rhizoctonia solani) subgroups AG-1-IE and AG-1-IF with molecular markers. Journal of General Plant Pathology, 78(2), 85-98.
13. Gonzalez, N., Beaver, J., Rosas, J. C., Godoy-Lutz, G., & Steadman, J. (2008). Development of a differential set of common bean lines to screen for web blight pathogen virulence. Annual Report-Bean Improvement Cooperative, 51, 32.
14. Guillemaut, C., Edel-Hermann, V., Camporota, P., Alabouvette, C., Richard-Molard, M., & Steinberg, C. (2003). Typing of anastomosis groups of Rhizoctonia solani by restriction analysis of ribosomal DNA. Canadian Journal of Microbiology, 49(9), 556-568.
15. Hall, R. (1994). Bean diseases, bean pathogens, bean disease control. Compendium of Bean Diseases, APS press, Minnesota, USA.
16. Karaca, G. H., Ozkoc, I., & Erper, I. (2002). Determination of the anastomosis grouping and virulence of Rhizoctonia solani Kühn isolates associated with bean plants grown in Samsun/Turkey. Pakistan Journal of Biological Sciences, 5(4), 434-437.
17. Kılıçoğlu, M. Ç., & Özkoç, İ. (2010). Molecular characterization of Rhizoctonia solani AG4 using PCR-RFLP of the rDNA-ITS region. Turkish Journal of Biology, 34(3), 261-269.
18. Kılıçoğlu, M. Ç., & Özkoç, İ. (2013). Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8 S rDNA. Turkish Journal of Biology, 37(1), 18-24.
19. Mora-Umaña, F., Barboza, N., Alvarado, R., Vásquez, M., Godoy-Lutz, G., Steadman, J. R., & Ramírez, P. (2013). Virulence and molecular characterization of Costa Rican isolates of Rhizoctonia solani from common bean. Tropical Plant Pathology, 38(6), 461-471.
20. Muyolo, N. G., Lipps, P. E., & Schmitthenner, A. F. (1993). Anastomosis grouping and variation in virulence among isolates of Rhizoctonia solani associated with dry bean and soybean in Ohio and Zaire. Phytopathology, 83(4), 438-444.
21. Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press: New York, USA.
22. Schmit, V., & Baudoin, J. P. (1992). Screening for resistance to Ascochyta blight in populations of Phaseolus coccineus L. and P. polyanthus Greenman. Field Crops Research, 30(1-2), 155-165.
23. Sneh, B., S. Jabaji-Hare, S. Neate & G. Dijst. (1996). Rhizoctonia Species: Taxonomy, molecular biology, ecology, pathology and disease control. Kluwer Academic Publishers, Netherlands.
24. Spedaletti, Y., Aparicio, M., Mercado Cárdenas, G., Rodriguero, M., Taboada, G., Aban, C., ... & Galván, M. (2016). Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina. Journal of Phytopathology, 164(11-12), 1054-1063.
25. Takegami, J. C., Beaver, J. S., Godoy-Lutz, G., Echávez-Badel, R., & Steadman, J. R. (2004). Inheritance of web blight resistance in common bean. The Journal of Agriculture of the University of Puerto Rico, 88(1-2), 45-54.
26. Valentín Torres, S., Vargas, M. M., Godoy-Lutz, G., Porch, T. G., & Beaver, J. S. (2016). Isolates of Rhizoctonia solani can produce both web blight and root rot symptoms in common bean (Phaseolus vulgaris L.). Plant Disease, 100(7), 1351-1357.
27. Van Schoonhoven, A., & Pastor-Corrales, M.A. (1987). Standard system for the evaluation of bean germplasm. Cali Colombia, CIAT.