Distribution and Genetic diversity of Prune dwarf virus (PDV) in Peach Growing Areas in Bursa and Bilecik Provinces

Abstract

Prune dwarf virus (PDV) is one of the important viral pathogens of stone fruits. In this study, PDV infection was investigated extensively for the first time in Bursa and Bilecik provinces, which have large peach production areas of Turkey. Four hundred-sixty peach leaves showing viral symptoms were subjected to DAS-ELISA test and 19 samples (%4.13) were found to be infected with PDV. As a result of RT-PCR studies, 862 bp length nucleotide sequences of the coat protein region of the virus genome were obtained and compared with reference isolates in the GenBank. Sequence comparisons showed 87.77-97.56% nucleotide identity. The genetic relationship was determined by phylogenetic analysis of Turkey and world isolates of PDV. The results of the study showed that there was no phylogenetic relationship between geographic origin and PDV isolates from different Prunus species. As a result of this study, nucleotide sequences of the coat protein regions of PDV isolates from peach were obtained for the first time in our country.

Keywords

• Bursa,Bilecik,peach,PDV,infection

Bursa ve Bilecik İlleri Şeftali Yetiştiriciliği Yapılan Alanlarda Prune dwarf virus (PDV) Yaygınlığı ve Genetik Çeşitliliği

Öz

Prune dwarf virus (PDV), sert çekirdekli meyve ağaçlarının önemli viral patojenleri arasında yer almaktadır. Bu çalışmada Türkiye’nin önemli şeftali üretiminin yapıldığı Bursa ve Bilecik illerinde ilk kapsamlı PDV enfeksiyonu araştırılmıştır. 460 viral simptom şüphesi ile toplanan şeftali yaprağı DAS-ELISA testine tabi tutulmuş ve 19 örneğin (%4.13) PDV ile enfekteli olduğu tespit edilmiştir. Yapılan RT-PCR çalışmaları sonucunda virüsün kılıf protein bölgesine ait 862 bp uzunluğunda nükleotid dizileri elde edilmiş, elde edilen diziler GenBank’ta kayıtlı Türkiye ve dünya PDV referans izolatları ile karşılaştırılmıştır. Yapılan BLASTn analizi sonucunda PDV şeftali Türkiye izolatları, referans PDV sekans verileri ile nükleotid düzeyinde %87.77-97.56 arasında benzerlik göstermiştir. Nükleotid dizi bilgisi elde edilen izolatların filogenetik analiz çalışmaları ile, dünya ve Türkiye izolatları ile ilişkisi belirlenmiştir. Araştırma bulguları coğrafik orijin ve farklı Prunus türlerinden izole edilen PDV izolatları arasında herhangi bir filogenetik ilişkinin olmadığını göstermiştir. Çalışma sonucunda ilk kez ülkemizde şeftali PDV izolatlarının kılıf protein bölgesine ait nükleotid dizileri elde edilmiştir. 

Anahtar Kelimeler

• Bursa,Bilecik,şeftali,PDV,enfeksiyon

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