Thiadiazuron ve Zeatin’in Helichrysum pallasii’de Bitki Rejenerasyonuna Etkisi

Öz

Helichrysum cinsi, tedavi edici etkileri olan ve halk hekimliğinde kullanılan birçok türü içermektedir. H. pallasii yörede geleneksel tıp ve süs amaçlı kullanılan bu türlerden bir tanesidir. Bu nedenle, bu türler için doku kültüründe alternatif mikroçoğaltım protokollerinin ve sekonder metabolit üretiminin optimize edilmesi gerektirmektedir. Bu çalışmada Zeatin (ZEA) ile kombine edilmiş Thidiazuran (TDZ)’ nın H. pallasi'de sürgün rejenerasyonu üzerine etkileri incelenmiştir. İn vitro koşullarda çimlendirilen bitkiciklerden alınan yaprak, gövde ve kök kısımları eksplant olarak kullanılmıştır. Sonuç olarak, en yüksek bitki rejenerasyonu kök eksplantlarına uygulanan 1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (%88.9) ve 1 mg L-1 TDZ (%85.7) uygulamalarından elde edilmiştir. En düşük bitki rejenerasyon yüzdesi (%16.7) ise yaprak eksplantları kullanılan kontrol uygulamasından elde edilmiştir.

Anahtar Kelimeler

• ölmez çiçek
• mikro çoğaltım
• doku kültürü
• Thidiazuran
• Zeatin

Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii

Abstract

The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one of these species, used in the region for traditional medicine and ornamental purposes. Therefore, optimizing alternative micropropagation protocols of tissue culture conditions and secondary metabolite production for these species needs attention. The effect of Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot regeneration in H. pallasi was investigated during this study. The leaf, stem and root parts taken from the seedling germinated under in vitro conditions were used as explants. As a result, the root explants were more efficient compared to other explants in inducing plant regeneration using 1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (88.9%) and 1 mg L-1 TDZ treatment (85.7%). The lowest plant regeneration percentage (16.7%) was found in control medium using leaf explants.

Keywords

• immortal flower
• micropropagation
• tissue culture
• Thidiazuran
• Zeatin

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