Effects of Different Biological Fertilizers Formulated with Multiple Bacteria and Carriers in Pazar 20 Tea Clone on Leaf Enzyme Activity

Abstract

This study was performed on 2-year-old seedlings of the Pazar-20 tea clone pots in 2014-2015. Twelve different bacterial isolates (Bacillus atrophaeusRC11, Bacillus megateriumRC07, Bacillus megaterium42/4, Bacillus megaterium21/3, Pseudomonas fluorescensRC77, Pseudomonas fluorescens 8/4, Pseudomonas fluorescens8/6, Pseudomonas fluorescens9/7, Bacillus subtilisRC63, Bacillus subtilis 39/3, Bacillus subtilis36/10, Bacillus subtilisRC521), which were isolated from acidic tea soils and characterized and all laboratory tests were performed. With biological fertilizers formed by triple combinations and 7 different carrier formulations (K-tea compost, T-peat, P-perlite, L-leonardite, Z-zeolite, V-vermiculite and S-liquid carrier formulation) on the enzyme activities (peroxidase-POD, Polyphenol oxidase-PPO, Urease, 5-Dehydroxyshikimate reductase, Alchol dehydrogenase-ADH, Glucose 6-phosphate dehydrogenase-G6PD, 6-phosphogluconate dehydrogenase-6PGD, Glutathione reductase-GR and Glutathione S-transferase-GST) of tea leaves were determined. All bacterial formulations used in the study positively affected the enzyme activities in the tea leaves at different rates compared to the control. This activity was found to be statistically significant. Additionally, it was determined that the efficiency of the carrier was important in both years (2014-2015) statistically, liquid formula and peat carriers had the highest effect.

Keywords

• Camellia sinensis
• PGPR formulas
• enzym activity
• carriers

Çoklu Bakterilerle Oluşturulmuş Farklı Biyolojik Gübre Formulasyonlarının Pazar 20 Çay Klonunda Yaprak Enzim Aktivitesine Etkileri

Öz

Bu çalışma, 2014-2015 yıllarında potlarda, Pazar-20 çay klonuna ait 2 yaşlı fidanlarda yürütülmüştür. Asidik çay topraklarından izole edilerek tanılanan ve karakterize edilip tüm laboratuvar testleri yapılan, biyolojik gübre adaylarından 12 farklı bakteri izolatının (Bacillus atrophaeusRC11, Bacillus megateriumRC07, Bacillus megaterium42/4, Bacillus megaterium21/3, Pseudomonas fluorescen RC77, Pseudomonas fluorescens8/4, Pseudomonas fluorescens8/ 6, Pseudomonas fluorescens9/7, Bacillus subtilisRC63, Bacillus subtilis39/3, Bacillus subtilis36/10, Bacillus subtilisRC521) üçerli kombinasyonları ile oluşturulmuş biyolojik gübre formulasyonları ile 7 farklı taşıyıcının (K-çay kompostu, T-torf, P-perlit, L-leonardit, Z-zeolit, V-vermikülit ve S-likit taşıyıcı formulasyonu) birlikte uygulanmalarının çay yapraklarının enzim aktivitelerine (Peroksidaz-PO, Polifenol oksidaz- PPO, Üreaz, 5-Dehidroksişikimat Redüktaz, Alkol dehidrogenaz-ADH, Glikoz 6 fosfat dehidrogenaz-G6PD, 6 Fosfo glukonat dehidrogenaz-6PGD, Glutatyon redüktaz-GR, Glutatyon S-transferaz-GST) etkileri belirlenmiştir. Araştırmada kullanılan tüm bakteriyel formulasyonlar, çay yaprağındaki enzim aktivitelerini kontrole kıyasla farklı oranlarda olumlu yönde etkilemiştir. Bu etkinlik istatistiki olarak önemli bulunmuştur. Ayrıca, yapılan değerlendirmelerde, her iki yılda da (2014-2015) taşıyıcıların da etkinliğinin önemli olduğu, likit formüllü taşıyıcı ile torf olarak kullanılan taşıyıcıların en yüksek etkiye sahip oldukları belirlenmiştir.

Anahtar Kelimeler

• Camellia sinensis L.,
• PGPR fomülleri
• enzim aktivitesi
• taşıyıcılar

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