Species Identification of Small Mammal Fauna in Bilecik Province and Molecular Researches for the Protection of Gene Resources

Year 2019, Volume: 5 Issue: 1, 149 - 160, 30.06.2019

Tuba Yağcı

https://doi.org/10.24180/ijaws.478168

Cited By: 2

Abstract

In this study, it was aimed to
determine the ecological, karyological and morphological properties of the
fauna of small mammals from Bilecik province in between 2016-2017 years.
Animals were identified by analyzing their nest structure, chromosomal feature
and pelage color accordingly species-specific characteristics were recorded.
The chromosome number and structure are presented as ideograms. In total 9
species were recorded, in which Mus
musculus domesticus, Rattus rattus,
Microtus lydius, Crocidura suaveolens species
were the first data in Bilecik province. For the construction of cDNA’s, RNA
isolation was performed from the blood samples of these identified animals.
These cDNA’s were stocked for subsequent molecular studies. According to the
field work the most sufficient habitats for small mammals were determined as
forest coast and cultivated lands in Bilecik province.

Keywords

Small mammalian fauna, Karyology, cDNA, Bilecik, Turkey

Bilecik İli Küçük Memeli Faunasının Tür Tespiti ve Gen Kaynaklarının Korunmasına Yönelik Moleküler Araştırmalar

Abstract

Bu araştırmada
2016-2017 yılları arasında Bilecik ili küçük memeli faunasının ekolojik,
karyolojik ve morfolojik olarak belirlenmesi amaçlanmıştır. Hayvanların
teşhisinde yuva yapısı, kürk rengi ve kromozomal özellikler incelenerek türe
özgü karakteristikler kaydedilmiştir. Kromozom sayı ve yapıları ideogramlar
halinde sunulmuştur. Araştırma sonucunda tespit edilen Mus musculus domesticus, Rattus
rattus, Microtus lydius, Crocidura suaveolens Bilecik ili için ilk veriler olup toplam 9 tür kaydı
verilmiştir. Tür tespiti yapılan hayvanlara ait kan örneklerinden cDNA
oluşturulmak üzere RNA izolasyonu yapılmıştır. Elde edilen cDNA’lar daha
sonraki moleküler araştırmalarda kullanılmak üzere stoklanmıştır. Bilecik
ilinde yapılan arazi çalışmalarına göre küçük memeliler için en elverişli
habitatlar ekili araziler ve orman kıyısı alanlarıdır. 

Keywords

Küçük memeli faunası, Karyoloji, cDNA, Bilecik, Türkiye

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