Genetic Variation of Fusarium spp. Isolates Associated with Root and Crown Rot of Winter Wheat Using Retrotransposon-Based iPBS Assays

Year 2019, Volume: 5 Issue: 2, 250 - 259, 13.12.2019

Mehtap Alkan Mehmet Erhan Göre Harun Bayraktar Göksel Özer

https://doi.org/10.24180/ijaws.537423

Cited By: 5

Abstract

Genetic variation among the
isolates of Fusarium spp., causal agent of root and crown rot of
winter wheat, was evaluated using retrotransposons-based inter-primer
binding site (iPBS) markers in this study. Thirty-two isolates were
isolated from diseased wheat plants obtained wheat growing areas in Turkey and
Azerbaijan in 2017 and 2018, for this purpose. Among the isolates, F. culmorum was the most commonly found
with 23 isolates, followed by F.
pseudograminearum with five isolates, and F. graminearum with four isolates. The seven iPBS retrotransposon
markers produced 114 bands, of which 99 were polymorphic (86.8%) with an
average of 14.14 polymorphic bands per primer for the isolates. The polymorphism
information contents (PIC) per markers ranged from 0.10 to 0.29 with the
average being 0.17. The dendrogram derived from unweighted pair group method
with arithmetic mean (UPGMA) cluster analyses based on the data of iPBS markers
divided the isolates into three clusters in according to their taxonomic
grouping at species level and their origin country in the groups. Population
structure was estimated based on Bayesian modeling and the results showed three
populations (K = 3) supporting the
clustering of isolates in the dendrogram with the highest mean value of Ln
likelihood of data (-476.0). Utilization of the iPBS markers produced high
level of polymorphism at the interspecies level, which allows for the
separation of species. This is the first study on genetic diversity and
population structure of Fusarium spp.
isolates on wheat using iPBS markers.

Keywords

Wheat, Fusarium spp., iPBS markers, retrotransposons

Kışlık Buğdaylarda Kök ve Kökboğazı Çürüklüğüne Sebep Olan Fusarium spp. İzolatları Arasındaki Genetik Varyasyonun Retrotranzpozon Temelli iPBS Markörleri ile İncelenmesi

Abstract

Bu çalışmada,
kışlık buğdaylarda kök ve kökboğazı çürüklüğüne sebep olan farklı Fusarium türlerine ait izolatlar arasındaki genetik çeşitlik,
retrotranspozon temelli olan primerler arası bağlanma bölgesi (iPBS) markörleri
kullanılarak incelenmiştir. Bu amaçla, 2017 ve 2018 yıllarında Türkiye ve
Azerbaycan buğday yetiştirme alanlarından 32 Fusarium türü izolat elde
edilmiştir.  İzolatlar arasında en yaygın
bulunan tür 23 izolat ile F. culmorum
olarak belirlenmiş, bunu beş izolat ile F.
pseudograminearum ve dört izolat ile F.
graminearum takip etmiştir. Yedi iPBS retrotranspozon markörü izolatlar için
99 adeti polimorfik (%86.8) olmak üzere 114 bant üretmiş, primer başına
ortalama 14.14 polimorfik bant elde edilmiştir. Markörlerin polimorfik bilgi
içeriği (PIC) ortalama 0.17 olup, bu değer 0.10 ile 0.29 arasında değişmiştir. iPBS
markörleri ile elde edilen verilere dayalı ağırlıklı olmayan aritmetik ortalama
eş grup metodu (UPGMA) küme analizlerinin sonrasında elde edilen dendrogramda,
izolatlar tür seviyesinde ile üç gruba ayrılmıştır. Ayrıca grup içerisinde yer
alan izolatlar ise orijini olan ülkeye göre dağılım sergilemiştir. Popülasyon
yapısı, Bayesian modellemesine dayanılarak hesaplanmış ve sonuçlar
dendrogramdaki izolatların kümelenmesini destekleyecek şekilde üç popülasyona
ayrılmış (K = 3) ve Ln olasılığının
en yüksek ortalama değeri (-476.0) eşlik etmiştir. iPBS markörlerinin
kullanılması, türler arası seviyede oldukça yüksek düzeyde polimorfizm üretmiş
olup türlerin birbirinden ayrılmasını sağlamıştır. Çalışma, buğdaydan elde
edilmiş Fusarium spp. izolatlarının
genetik çeşitliliğini ve popülasyon yapısını iPBS markörleri analizi ile ortaya
koyan ilk çalışma olma özelliği taşımaktadır. 

Keywords

Buğday, Fusarium spp., iPBS markörleri, Retrotranspozon

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