Effects of Azadirachtin and Spinosad on the Survival and Virulence of Some Local Entomopathogenic Nematodes Isolates

Year 2019, Volume: 5 Issue: 2, 280 - 285, 13.12.2019

Ebubekir Yüksel , Ramazan Canhilal , Mustafa İmren

https://doi.org/10.24180/ijaws.591287

Cited By: 4

Abstract

Entomopathogenic nematodes (EPN) are often used in
combination with biopesticides in biologics control applications. Determination
of the toxic effects of biopesticides on EPNs is crucial for achieving the desired
results from these applications. The effects of commonly used biopesticides
[Nimiks (40 g L^-1 Azadirachtin) and Laser (Spinosad 480 g L^-1)]
on the survival and virulence of some local EPN species (Steinernema feltiae E-76, S.
littorale MGZ-4-S, Heterorhabditis
indica 216-H) were investigated on the last larval instar of Galleria mellonella L. (Lepidoptera:
Pyralidae) larvae in 24 and 48 hours periods in laboratory conditions (25^oC±2,
R.H.  %65±5). Of the EPN species directly
exposed to the highest recommended doses of Spinosad and Azadirachtin, the most
tolerant species was H. indica 216-H
with the survival rate of 94% during the first 24 hours. Steinernema littorale MGZ-4-S was the most susceptible species to
Azadirachtine with the mortality rate of 11% and 12% respectively in 24 and 48
hours periods. Mortality rates of the tested EPN species on the G. mellonella larvae ranged from 80% to
100%. The obtained results revealed that the EPN species tested are compatible
with Spinosad and Azadirachtin under laboratory conditions.

Keywords

Biopesticide, Azadirachtin, Spinosad, Entomopathogenic, Nematode

Azadirachtin ve Spinosadın Bazı Yerel Entomoapatojen Nematod İzolatlarının Canlılığı ve Virülensliği Üzerine Etkileri

Abstract

Entomopatojen nematodlar (EPN), biyoloijk mücadele
uygulamaları içerisinde çoğu zaman biyopestisitlerle birlikte kullanılmaktadır.
Biyopestisitlerin EPN’lar üzerindeki toksik etkilerinin belirlenmesi, bu
uygulamalardan istenilen başarının elde edilebilmesi için oldukça önemlidir. Bu
çalışmada yaygın bir şekilde kullanılan Nimiks (40 g L^-1 Azadirachtin) ve
Laser (Spinosad 480 g L^-1)
biyopestisitlerinin bazı yerel EPN türlerinin (Steinernema feltiae E-76, Heterorhabditis
indica 216-H, S. littorale
MGZ-4-S) hayatta kalma ve virülensliği üzerindeki etkileri 24 ve 48 saatlik
periyotlarda son dönem Galleria
mellonella larvaları üzerinde laboratuvar koşullarında (25^oC±2,
R.H. %65±5) araştırılmıştır. Spinosad ve Azadirachtinin tavsiye edilen en
yüksek uygulama dozlarına doğrudan maruz kalan EPN türleri içerisinde ilk 24
saatlik periyotta en tolerant türün %94 canlılık oranıyla H. indica 216-H olduğu belirlenmiştir. Steinernema littorale MGZ-4-S izolatının Azadirachtine 24 ve 48
saat periyotlarda sırasıyla %11 ve %12 ölüm oranlarıyla en çok duyarlılık
gösteren tür olmuştur. Test edilen EPN türlerinin son dönem G. mellonella larvaları üzerinde meydana
getirdikleri ölüm oranları %80 ile %100 arasında değişmiştir. Elde edilen
veriler, test edilen EPN türlerinin Spinosad ve Azadirachtin ile laboratuvar
şartlarında uyumlu olduğunu göstermektedir.

Keywords

Biyopestisit, Azadirachtin, Spinosad, Entomopatojen, Nematod

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