Mitigation of Salinity Stress on Chickpea Germination by Salicylic Acid Priming

Year 2020, Volume: 6 Issue: 3, 582 - 591, 21.12.2020

Mustafa Ceritoğlu Murat Erman

https://doi.org/10.24180/ijaws.774969

Cited By: 11

Abstract

Salinity is a global problem that threatens chickpea (Cicer arietinum L.) cultivation in all stages from germination to maturity. Also, salt-affected areas in the World are increasing by the day. Germination is one of the primary stages affecting plant growth and seed yield. Thus, germination performance of seeds has a vital role in vegetative and productive stages. Seed priming, a cheap and easily applicable technique, improves the germination performance by regulating enzymatic reactions and controlling water intake. Seed priming is controlled hydration of seeds soaked in a solution that has low osmotic potential to start the germination metabolism without elongation of radical. The aim of the study was to investigate the effects of different priming treatments on germination traits of chickpea under saline conditions. Three levels of salinity (control, 50 mM and 100 mM NaCl) and 5 priming treatments (non-primed, hydro-priming, 0.1 mM, 0.2 mM and 0.3 mM salicylic acid) were used in the study. Germination percentage (GP), mean germination time (MGT), germination rate (GR), germination index (GI) and coefficient of uniformity of germination (CUG) were varied between 80.7-98.3%, 1.40-2.67 day, 38.1-75.7, 37.5-72.5 and 0.3-0.7, respectively. Although all treatments caused significant positive effects, the 0.2 mM SA mostly improved the germination traits in the study. Also, it was determined that 0.2 mM salicylic acid priming is the threshold for chickpea and higher concentrations have inhibitory effects on the germination process.

Keywords

Hydro-priming, Osmo-priming, Ion toxicity, Stress alleviation, Uniformity of germination

Nohut Çimlenmesi Üzerine Tuzluluk Stresinin Salisilik Asit Priming ile Azaltılması

Abstract

Tuzluluk, nohut (Cicer arietinum L.) tarımını çimlenmeden olgunluğa kadar her aşamada tehdit eden küresel bir sorundur. Ayrıca, yeryüzünde tuzluluktan etkilenen alanlar giderek artmaktadır. Çimlenme ürün kalitesini ve tane verimini etkileyen evrelerin başında gelir. Bu nedenle, tohumların çimlenme performansları vejetatif ve generatif gelişim dönemleri açısından hayati önem taşımaktadır. Ucuz ve kolay uygulanabilir bir teknik olan priming, su alımını kontrol ederek ve enzimatik reaksiyonları düzenleyerek çimlenme performansının artmasını sağlar. Priming, düşük osmotik potansiyele sahip bir çözeltide bekletilerek tohumun çimlenme metabolizmasının kökcük çıkışı olmaksızın başlatıldığı kontrollü su alımını ifade eder. Çalışmanın amacı, tuzluluk stresi altındaki nohutta farklı priming uygulamalarının çimlenme özellikleri üzerine etkilerini belirlemektir. Çalışmada 3 tuzluluk seviyesi (kontrol, 50 mM NaCl ve 100 mM NaCl) ve 5 ekim öncesi uygulama (kontrol, hidro-priming, 0.1, 0.2 ve 0.3 mM salisilik asit) kullanılmıştır. Çimlenme yüzdesi (% 80.7-98.3), ortalama çimlenme süresi (1.40-2.67 gün), çimlenme hızı (38.1-75.7), çimlenme indeksi (37.5-72.5) ve çimlenme üniformluk katsayısı (0.3-0.7) değerleri , , ve Belirlenmiştir. Çalışmadaki tüm uygulamalar önemli pozitif etkilere sebep olmasına rağmen 0.2 mM SA uygulması çimlenme özelliklerini en çok iyileştiren uygulama olmuştur. Ayrıca, 0.2 mM SA uygulamasının nohutta eşik değer olduğu, daha yüksek konsantrasyonların çimlenme süreci üzerine engelleyici/azaltıcı etki gösterdiği tespit edilmiştir.

Keywords

Hidro-priming, Osmo-priming, İyon toksisitesi, stres azaltma, Çimlenme üniformitesi

References

• Aflaki, F., Sedghi, M., Pazuki, A., & Pessarakli, M. (2017). Investigation of seed germination indices for early selection of salinity tolerant genotypes: A case study in wheat. Emirates Journal of Food and Agriculture, 29(3), 222-226.
• Anonymous. (2020). Registry properties of Çakır chickpea cultivar. Available at: https://arastirma.tarimorman.gov.tr/gktaem/Belgeler/Tescilli%20%C3%87e%C5%9Fitlerimiz/Nohut/cakir.pdf. Access date: June 10, 2020.
• Asadi, M., Heidari, M. A., Kazemi, M., & Filinejad, A. R. (2013). Salicylic acid induced changes in some physiological parameters in chickpea (Cicer arietinum L.) under salt stress. Journal of Agricultural Technology, 9(2), 311-316.
• Azarnia, M., Biabani, A., Eisvand, H. R., Alamdari, E. G., & Safikhani, S. (2016). Effect of seed priming with gibberellic acid and salicylic acid on germination characteristic and seed and seedlings physiological quality of lentil (Lens culinaris L.). Iranian Journal of Seed Research, 3(1), 59-72.
• Bouallegue, A., Soissi, F., Nouairi, I., Souibgui, M., Abbes, Z., & Mhadhbi, H. (2017). Salicylic acid and hydrogen peroxide pretreatments alleviate salt stress in faba bean (Vicia faba) seeds during germination. Seed Science and Technology, 45(3), 675-690.
• Boukraa, D., Benabdelli, K., Belabid, L., & Bennabi, F. (2013). Effect of salinity on chickpea seed germination pre-treated with salicylic acid. Scientific Journal of Biological Sciences, 2(4), 86-93.
• Cha-Um, S., & Kirdmanee, C. (2010). Effect of glycine betaine on proline, water use, and photosynthetic efficiencies, and growth of rice seedlings under salt stress. Turkish Journal of Agriculture and Forestry, 34, 517-527.
• Chen, T., Pineda, I. M. G., Brand, A. M., & Stützel, H. (2020). Determining ion toxicity in cucumber under salinity stress. Agronomy, 10(5), 667.
• Ceritoglu, M., & Erman, M. (2019). Tane baklagillerin ekim nöbetinde kullanılması. VI. International Conference on Mathematic – Engineering- Natural & Medical Science, Adana.
• Ceritoglu, M., Erman, M., & Yildiz, F. (2020). Effect of salinity on germination and some agro-morfological traits in chickpea seedling. ISPEC Journal of Agricultural Science, 4(1), 82-96.
• Ellis, R. A., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9, 373-409.
• FAOSTAT. (2018). Chickpea production. Online database at https://www.fao.org/faostat/ en/#data. Access date: June 5, 2020.
• Farooq, M., Basra, S. M. A., Tauseef, M., Rehman, H., & Munir, H. (2007). Priming with ethanol, ascorbate and salicylicate enhances the germination and early seedling growth of pea (Pisum sativum L.). Pakistan Journal of Agricutural Science, 44(1), 30-39.
• Farooq, M., Hussain, M., Nawaz, A., Lee, D., Alghamdi, S. S., & Siddique, K. H. M. (2017). Seed priming improves chilling tolerance in chickpea by modulating germination metabolism, trehalose accumulation and carbon assimilation. Plant Physiology and Biochemistry, 111, 274-283.
• Francis, C., Jones, A., Crookston, K., Wittler, K., & Goodman, S. (1986). Strip cropping corn and grain legumes: A review. American Journal of Alternative Agriculture, 1(4), 159-164.
• Gan, Y., Zentner, R. P., McDonald, C. L., Warkentin, T., & Vandenberg, A. (2009). Adaptability of chickpea in northern high latitude areas—Maturity responses. Agricultural and Forest Meteorology, 149(3-4), 711-720.
• Gopalakrishnan, S., Pande, S., Sharma, M., Humayun, P., Kiran, B. K., Sandeep, D., Vidya, M. S., Deepthi, K., & Rupela, O. (2011). Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea. Crop Protection, 30(8), 1070-1078.
• Goharrizi, K. J., Amirmahani, F., & Salehi, F. (2020). Assessment of changes in physiological and biochemical traits in four pistachio rootstocks under drought, salinity and drought + salinity stresses. Physiologia Plantarum, 168, 973-989.
• Grewal, H. S. (2010). Water uptake, water use efficiency, plant growth and ionic balance of wheat, barley, canola and chickpea plants on a sodic vertosol with variable subsoil NaCl salinity. Agricultural Waste Management, 97(1), 148-156.
• Haider, M. U., Hussain, M., Farooq, M., & Nawaz, A. (2020). Optimizing zinc seed priming for improving the growth, yield and grain biofortification of mungbean (Vigna radiata (L.) wilczek). Journal of Plant Nutrition, 43(10), 1438-1446.
• Hajjarpoor, A., Vadez, V., Soltani, A., Gaur, P., Whitbread, A., Babu, D. S., Gumma, M. K., Diancoumba, M., & Kholova, J. (2018). Characterization of the main chickpea cropping systems in India using a yield gap analysis approach. Field Crops Research, 223, 93-104.
• Hubbard, M., Germida, J., & Vujanovic, V. (2012). Fungal endophytes improve wheat seed germination under heat and drought stress. Botany, 90, 137-149.
• Janda, T., Horváth, E., Szalai, G., & PáLdi, E. (2007). Role of Salicylic Acid in the Induction of Abiotic Stress Tolerance. In: Hayat S., Ahmad A. (eds) Salicylic Acid: A Plant Hormone. Dordrecht: Springer. https://doi.org/10.1007/1-4020-5184-0_5
• Jatana, B. S., Ram, H., & Gupta, N. (2020). Application of seed and foliar priming strategies to improve the growth and productivity of late sown wheat (Triticum aestivum L.). Cereal Research Communications.
• Kader, M. A. (2015). A comparison of seed germination calculation formulae and the associated ınterpretation of resulting data. Journal and Proceedings of the Royal Society of New South Wales, 138, 65-75.
• Kaya, M., Kaya, G., Kaya, M. D., Atak, M., Sağlam, S., Khawar, K. M., & Çiftçi, C. Y. (2008). Interaction between seed size and NaCl on germination and early seedling growth of some Turkish cultivars of chickpea (Cicer arietinum L.). Journal of Zhejiang University Science B, 9(5), 371-377.
• Kim, S., Rayburn, A. L., Voigt, T., Parrish, A., & Lee, D. K. (2012). Salinity effects on germination and plant growth of prairie cordgrass and switchgrass. BioEnergy Research, 5, 225-235.
• Labouriau, L. G. (1983). A germinação das sementes. Organização dos Estados Americanos. Programa Regional de Desenvolvimento Científico e Tecnológico. Série de Biologia. Monografia.
• Lavrenko, S. O., Lavrenko, N. M., & Lykhovyd, P. V. (2019). Effect of degree of salinity on seed germination and initial growth of chickpea (Cicer arietinum). Biosystem Diversity, 27(2), 101-105.
• Maguire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2, 176–177.
• Matthews, S., & Khajeh-Hosseini, M. (2007). Length of the lag period of germination and metabolic repair explain vigour differences in seed lots of maize (Zea mays). Seed Science and Technology, 35(1), 200-213.
• Maurya, D. K., Hasanain, M., Verma, S. K., Singh, P. K., Kumar, V., Singh, S., & Mishra, R. (2020). Seed priming and its effect on enhancing pulse productivity. Food and Scientific Reports, 1, 20-22.
• Mohtashami, M., Naderi, A., Ghanbari, A. A., Alavifazel, M., & Lak, S. (2016). Effect of seed pre-treatment with growth regulators on seed yield and yield components of common bean (Phaseolus vulgaris L.). Turkish Journal of Field Crops, 21(2), 313-317.
• Özaktan, H., Çiftçi, C. Y., Kaya, M. D., Uzun, S., Uzun, O., & Akdogan, G. (2018). Chloride salts inhibit emergence and seedling growth of chickpea rather than germination. Legume Research, 41(1), 60-66.
• Pakmehir, A., Rastgoo, M., Shekari, F., Saba, J., Vazayefi, M., & Zangani, A. (2011). Effect of salicylic acid priming on yield and yield components of cowpea (Vigna unguiculata L.) under water deficit at reproductive stage. Iranian Journal of Pulse Research, 2(1), 53-64.
• Piovan, M. J., Pratolongo, P., Donath, T. W., Loydi, A., & Eckstein, L. (2019). Germination response to osmotic potential, osmotic agents, and temperature of five halophytes occurring along a salinity gradient. International Journal of Plant Science, 180(4), 345-355.
• Ranal, M. A., & De Santana, D. G. (2006). How and why to measure the germination process?. Revista Brasileira de Botanica, 29(1), 1-11.
• Reis, S., Pavia, I., Carvalho, A., Moutinho-Perreira, J., Correira, C., & Brito, J. L. (2018). Seed priming with iron and zinc in bread wheat: effects in germination, mitosis and grain yield. Protoplasma, 255, 1179-1194.
• Qin, J., Dong, W. Y., He, K. N., Yu, Y., Tan, G. D., Han, L., Dong, M., Zhang, Y. Y., Zhang, D., Li, A. Z., & Wang, Z. L. (2010). NaCl salinity-induced changes in water status, ion contents and photosynthetic properties of Shepherdia argentea (Pursh) Nutt seedlings. Plant, Soil and Environment, 56, 325-332.
• Roorkiwal, M., Jain, A., Kale, S. M., Doddamani, D., Chitikineni, A., Thudi, M., & Varshney, R. K. (2018). Development and evaluation of high-density Axiom®CicerSNP Array for high-resolution genetic mapping and breeding applications in chickpea. Plant Biotechnology Journal, 16, 890–901.
• Sakhabutdinova, A. R., Fatkhutdinova, D. R., Bezrukova, M. V., & Shakirova., F. M. (2003). Salicylic acid prevents damaging action of stress factors on wheat plants. Bulgarian Journal of Plant Physiology, Special Issue, 314-319.
• Scott, S. J., Jones, R. A., & Williams, W. A. (1984). Review of data analysis methods for seed germination. Crop Science, 24, 1192-1199.
• Shahid, S. A., Zaman, M., & Heng, L. (2018). Soil Salinity: Historical perspectives and a world overview of the problem. In: Guideline for Salinity Assessment, Mitigation and Adaptation Using Nuclear and Related Techniques. Cham: Springer.
• Shakirova, F. M., Sakhabutdinova, A. R., Bezrukova, M. V., Fatkhutdinova, R. A., & Fatkhutdinova, D. R. (2003). Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164(3), 317-322.
• Shapiro, S. S., & Wilk, M. B. (1965). An analysis of variance test for normality (complete samples). Biometrika, 52(34), 591-611.
• Sher, A., Sarwar, T., Nawaz, A., Ijaz, M., Sattar, A., & Ahmad, S. (2019). Methods of seed priming. Priming and Pretreatment of seeds and seedling, Singapore: Springer.
• Shoor, M., Afrousheh, M., Rabeie, J., & Vahidi, M. (2014). The effect of salinity priming on germination and growth stage of cumin (Cuminum cyminum L.). Research Journal of Agricultural and Environmental Management, 3(7), 340-352.
• Singla, R., & Garg, N. (2005). Influence of salinity on growth and yield attributes in chickpea cultivars. Turkish Journal of Agriculture and Forestry, 29, 231-235.
• Soe, K. M., Htwe, A. Z., Moe, K., Tomomi, A., & Yamakawa, T. (2020). Diversity and effectivity of indigenous Mesorhizobium strains for chickpea (Cicer arietinum L.) in Myanmar. Agronomy, 10(2), 287.
• Soleymani, A., & Shahrajabian, M. H. (2018). Changes in germination and seedling growth of different cultivars of cumin to drought stress. Cercetări Agronomice în Moldova, 1(173), 91-100.
• Souhail, M., & Chaabane, R. (2009). Toxicity of the salt and pericarp ınhibition on the germination of some Atriplex species. American-Eurasian Journal of Toxicological Sciences, 1(2), 43-49.
• Soysal, S., Uçar, Ö., & Erman, M. (2020). The effects of different plant densities on yield and some yield characteristics of chickpea (Cicer arietinum L.) on the ecological conditions of Siirt province. EJONS International Journal on Mathematics, Engineering and Natural Sciences, 4(15), 785-795.
• Thomas, S., Anand, A., Chinnusamy, V., Dahaju, A., & Basu, S. (2013). Magnetopriming circumvents the effect of salinity stress on germination in chickpea seeds. Acta Physiologiae Plantarum, 35, 3401-3411.
• Ullah, A., Farooq, M., Hussain, M., Ahmad, R., & Wakeel, A. (2019). Zinc seed priming improves stand establishment, tissue zinc concentration and early seedling growth of chickpea. The Journal of Animal and Plant Science, 29(4), 1046-1053.
• Xing, Q., Dekker, S., Kyriakopoulou, K., Boom, R. M., Smid, E. J., & Schutyser, M. A. I. (2020). Enhanced nutritional value of chickpea protein concentrate by dry separation and solid state fermentation. Innovative Food Science and Emerging Technologies, 59, 102269.
• Varier, A., Vari, A. K., & Dadlani, M. (2010). The subcellular basis of seed priming. Current Science, 99, 450-456.
• Varshney, R. K, Thudi, M., Nayak, S. N., Gaur, P. M., Kashiwagi, J., Krishnamurthy, L., Jaganathan, D., Koppolu, J., Bohra, A., Tripathi, S., Rathore, A., Jukanti, A. K., Jayalakshmi, V., Vemula, A., Singh, S. J., Yasin, M., Sheshshayee, M. S., & Viswanatha, K. P. (2014) Genetic dissection of drought tolerance in chickpea (Cicer arietinum L.). Theoretical and Applied Genetics, 127, 445–462.
• Yildirim, E., Karlidag, H., & Turan, M. (2009). Mitigation of salt stress in strawberry by foliar K, Ca and Mg nutrient supply. Plant, Soil and Environment, 55, 213-221.
• Zapata, P. J., Serrano, M., Pretel, M. T., Amoros, A., & Botella, M. A. (2004). Polyamines and ethylene changes during germination of different plant species under salinity. Plant Science, 167(4), 781-788.
• Zhang, H., Irving, L. J., McGill, C., Matthew, C., Zhou, D., & Kemp, P. (2010.) The effects of salinity and osmotic stress on barley germination rate: sodium as an osmotic regulator. Annals of Botany, 106, 1027-1035.
• Zhu, G., An, L., Jiao, X., Chen, X., Zhou, G., & McLaughlin, N. (2019). Effects of gibberellic acid on water uptake and germination of sweet sorghum seeds under salinity stress. Chilean Journal of Agricultural Research, 79(3), 415-424.