In Vitro Propagation of Virus Indexed Gisela-5 (Prunus cerasus x Prunus canescens) - Clonal Cherry Rootstock

Abstract

In the present investigation, a technique for in vitro propagation of Gisela-5 (Prunus cerasus x Prunus canescens)- cherry rootstock has been developed.  Maximum in vitro establishment was achieved during the month of July and February. Treatment with 0.1 per cent HgCl₂ for 5 minutes was found to be the best for surface sterlization. Maximum in vitro establishment of explants (70%) was achieved on MS medium fortified with 0.5 mg/l BA and 0.5 mg/l GA_3.  Highest multiplication rate of 1:5 was observed on MS medium supplemented with five different concentrations and combinations of BA, GA₃, IBA and Kin. Shoot multiplication rate and shoot length showed an increase with the increase in number of subculturing passages which increased to a maximal of 1:9 and 6 cm after third and fourth passage. Best rooting of 18.20 per cent was observed in one step procedure whereas, maximum rooting (53.33%) was observed in two step procedure of rooting, Rooted plantlets were transplanted in sterilized sand for hardening and kept in the glasshouse, where 90 per cent survival was observed after 4 weeks of transfer. In vitro mother cultures were indexed for Cherry leaf roll virus, Apple chlorotic leaf spot virus and Prunus necrotic ring spot virus using DAS-ELISA procedure. All the tested samples showed negative results for the presence of these viruses, thus ensuring the production of healthy planting material.

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