In Vitro Propagation of Virus Indexed Gisela-5 (Prunus cerasus x Prunus canescens) - Clonal Cherry Rootstock

Abstract

In the present investigation, a technique for in vitro propagation of Gisela-5 (Prunus cerasus x Prunus canescens)- cherry rootstock has been developed.  Maximum in vitro establishment was achieved during the month of July and February. Treatment with 0.1 per cent HgCl₂ for 5 minutes was found to be the best for surface sterlization. Maximum in vitro establishment of explants (70%) was achieved on MS medium fortified with 0.5 mg/l BA and 0.5 mg/l GA_3.  Highest multiplication rate of 1:5 was observed on MS medium supplemented with five different concentrations and combinations of BA, GA₃, IBA and Kin. Shoot multiplication rate and shoot length showed an increase with the increase in number of subculturing passages which increased to a maximal of 1:9 and 6 cm after third and fourth passage. Best rooting of 18.20 per cent was observed in one step procedure whereas, maximum rooting (53.33%) was observed in two step procedure of rooting, Rooted plantlets were transplanted in sterilized sand for hardening and kept in the glasshouse, where 90 per cent survival was observed after 4 weeks of transfer. In vitro mother cultures were indexed for Cherry leaf roll virus, Apple chlorotic leaf spot virus and Prunus necrotic ring spot virus using DAS-ELISA procedure. All the tested samples showed negative results for the presence of these viruses, thus ensuring the production of healthy planting material.

Kaynakça

1. Buyukdemirci, H. 2008. The effects of medium ingredients on shoot propagation and rooting of cherry rootstocks in vitro. Acta Horticulturae, 795: 419-422.
2. Canli, F.A. and Demir, F. 2014. In vitro multiplication and rooting of ‘F12-1’ (Prunus avium L.) and ‘Maxma 14’ (Prunus mahaleb L. × P. avium L.) rootstocks. Indian Journal of Horticulture, 71 :145-150.
3. Clapa, D., Fira, A., Simu, M. and Horga, V.C. 2013 In vitro propagation of Gisela-5 cherry rootstock. Fruit Growing Research, 29: 100-105.
4. Clark, A.D. and Adams, A.S. 1977. Characteristics of the microplate method of ELISA for detection of plant viruses. Journal of General Virology, 34: 475-483.
5. Fidanci, A., Burak, M., Erenoglu, B. and Akçay, M.E. 2008. Determination of in vitro propagation techniques for some clonal cherry rootstocks. Acta Horticulturae, 795 :409-412.
6. Filiz, G., Fatma, K.and Bekir, S. 2010. The in vitro micropropagation of some clonal cherry rootstocks. Journal of Natural & Applied Sciences, 14 :144.
7. Gomez, K.A. and Gomez, A.A. 1984. Statistical Procedures for Agricultural Research. (John Wiley and Sons, New York)
8. Grant, N.J. and Hammatt, N. 1999. Increased root and shoot production during micropropagation of cherry and apple rootstocks: effect of subculture frequency. Tree physiology, 19 :899.

9. Hossini, A.D., Moghadam, E.G. and Anahid, S. 2010. Effects of media cultures and plant growth regulators in micropropagation of Gisela 6 rootstock. Annals of Biological Research, 1: 135-141.
10. Jones, O.P. and Hopgood, M.E. 1979. The successful propagation in vitro of two rootstocks of Prunus: the plum rootstock Pixy (P. insititia) and the cherry rootstock F12/1 (P. avium). Journal of Horticultural Sciences, 54:63-66.
11. Kaushal, N., Modgil, M., Thakur, M. and Sharma, D.R. 2005. In vitro clonal multiplication of an apple rootstock by culture of shoot apices and axillary buds. Indian Journal of Experimental Biology, 43: 561-565.
12. Marjanovic, D., Mededovic, S., Causevic, A. and Hadziselimovic, R. 2000. Micropropagation of wild cherry (Prunus avium L.) in vitro. Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 30: 35-42.
13. Minaev, V.A., Verzilin, A.V. and Vysostskii, V.A. 2003. Clonal micropropagation of low vigour clonal apple rootstocks bred at the Michurinsk State Agricultural University. Sadov-i-Vinog, 5 : 12-13.
14. Muna, A.S., Ahmad, A.K., Mahmoud, K. and Rahman, K.A. 1999. In vitro propagation of a semi-dwarfing cherry rootstock. Plant Cell, Tissue and Organ Culture, 59: 203-208.
15. Murashige, T. 1974. Plant propagation through tissue cultures. Annual Review of Plant Physiology, 25:135-166.
16. Nordstrom, A.C. and Eliasson, L. 1986 Uptake and translocation of C14-labled benzylaminopurine in apple shoots grown in vitro in relation to shoot development. Physiologia Plantarum, 68: 431-435.
17. Pevalek-Kozlina, B. and Jelaska, S. 1987. Microclonal propagation of Prunus avium. Acta Horticulturae, 212 :599-601.
18. Sarropoulou, V., Theriou, K.D. and Therios, I. 2014. L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture. Turkish Journal of Agriculture and Forestry, 38 : 887-897.
19. Sisko, M. 2011. In vitro propagation of Gisela 5 (Prunus cerasus × P. canescens). Agricultura,. 8 :31-34.
20. Sutter, E.G. 1996. General laboratory requirements, media and sterilization methods. In: Plant tissue culture concepts and laboratory Exercises edited by Trigiano RN & Gray DJ, (CRC Press, New York), 11-25.
21. Thakur, M., Sharma, D.R. and Kanwar, K. 2001. Mass micropropagation of Alnus nepalensis D. Don. Phytomorphology, 51: 123-127.
22. Xu, J., Kang, I.K., Kim, C.K., Han, J.S. and Choi, C. 2015.Optimization of apical tip culture condition for in vitro propagation of ‘Gisela 5’ dwarf cherry rootstock. Journal of Plant Biotechnology, 42 : 49-54.
23. Zamanipour, M., Moghadam, E.G., Tehranifar, A. and Abedi, B. 2015. The effects of media, plant growth regulators and apex size on the success of meristem culture in Prunus avium cv pishras-e-mashhad. Indian Journal of Fundamental and Applied Life Sciences, 5 : 924-929.