Antioxidant, Anticholinesterase and Antiproliferative Properties of the Wild Mushroom Hygrocybe Conica

Year 2025, Volume: 9 Issue: 2, 280 - 286

Aras Fahrettin Korkmaz

https://doi.org/10.32571/ijct.1779215

Abstract

In this study, antioxidant, anticholinesterase and antiproliferative properties of ethanol extracts of Hygrocybe conica were determined. The ethanol extract of the mushroom was extracted by Soxhlet apparatus. Antioxidant activities were determined by TAS, TOS, OSI, DPPH and FRAP analyses. The TAS value of the mushroom extract was 3.559 mmol/L, TOS value was 9.350 µmol/L and OSI value was 0.263. Radical scavenging and reducing capacities were also notable, with DPPH measured as 52.360 mg TE/g and FRAP as 78.503 mg TE/g. Anticholinesterase assays revealed moderate enzyme inhibition with IC₅₀ values of 169.29 µg/mL for AChE and 213.41 µg/mL for BChE, indicating lower activity compared to galantamine. The antiproliferative effect was evaluated in the A549 lung cancer cell line, where the extract significantly reduced cell viability in a dose-dependent manner, with the most pronounced inhibition observed at 200 µg/mL. These findings suggest that H. conica is a promising natural source of bioactive compounds with antioxidant, neuroprotective, and anticancer potential.

Keywords

Hygrocybe conica , antioxidant activity , anticholinesterase , antiproliferative effect , bioactive compounds

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