Production Of Hand Sterilizatıon Fluid Of Herbal Origin

Year 2019, Volume: 3 Issue: 2, 175 - 184, 16.12.2019

Sergen Cetebozan , Hatice Kubra Ozgul Sibel Yigitarslan

Abstract

When the body is exposed to
microorganisms of daily life, their negative effects on human health will be
observed. Industrial disinfection gels especially used for effective and rapid
hand disinfection, contain ethyl or isopropyl alcohol. Due to the presence of
alcohol, they cause some allergic reactions on the skin. The aim of this study
was to investigate the usability of flavonoids obtained from horse chestnut shells
as hand disinfection gel material. Single and multi-parameter optimizations were
applied to obtain the maximum amount of flavonoids from the material by
ultrasonic extraction. In single optimization, it was determined that
application of 50^oC, 2g-plant/100ml-water, 10 min-continuous sound
waves increased the extraction efficiency. To determine the optimum values ​​of
parameters in multiple optimization, Response-Surface Method including
Box-Benken design was used via Design-Expert program. As a result of the
numerical solution, application of ultrasonic extraction to 2.44g of horse
chestnut and 100ml of water at 40.1^oC for 14.89 minutes produced the
highest flavonoid content. The amount of antioxidant in extracts was analyzed
spectrophotometrically by DPPH method. Addition of 9.6ml of the extract to mixed
microorganism culture media resulted successful inhibition. Thus, it was
concluded that the disinfection liquid containg the same concentration produced
by thickening it with sodium arginate was found as an alternative to the
existing product containing synthetic chemicals.

Keywords

Aesculus hippocastanum, DPPH, Ultrasonic extraction

Supporting Institution

Suleyman Demırel University

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