Effects of Cadmium (Cd) on the Seed Germination and Growth of some selective Ethiopian Soybean (Glycine max L.) Genotypes

Abstract

The objective of this work was to evaluate the effects of cadmium (Cd) exposure on Soybean (Glycine max L.) plant germination and growth. This experiment was conducted at Biology Department laboratory and Biotechnology Department Green house, Wolkite University, Ethiopia, during the period of June to August 2019. The Petri dishes were treated with an equal volume of the different concentrations of CdCl2 solutions (0, 10,20,30,40 and 50 ppm). The seeds were allowed to germinate in the dark in an incubator at 25°C for 5 days and percentage of germination were recorded when the radicle reached 2 mm in length. In the pot culture experiment, Soybean were also analyzed on three different sampling (15, 30 and 45) days in soil added with various levels of cadmium similar with Petri dishes. The inner surfaces of the pot were lined with a polythene sheet and each pot contained 3kg of air dried soil. In each pot, six seeds of Soybean were sown and watered to field capacity. A maximum of three plants were thinned per each pots, after a week of germination and the effect of cadmium were tested at different levels of concentrations that decreased the germination and growth parameters such as root and shoot length compared with untreated plants.

Keywords

• Cadmium
• Genotype
• Growth
• Seed Germination
• Soybean

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