Tuz Stresi Koşullarında Prolin Uygulamalarının Biber Tohumlarının Çimlenmesi Üzerine Etkileri

Öz

Bu çalışmada, prolin uygulamalarının 75 mM NaCl ile oluşturulan tuz stresi altında Capsicum annuum L. (sivri biber) tohumlarının çimlenme özellikleri üzerine etkileri araştırılmıştır. Denemede, prolinin 0 (kontrol), 5 mM, 10 mM ve 15 mM olmak üzere dört farklı konsantrasyonu kullanılmış ve tohumlar bu solüsyonlarda 24 saat süreyle bekletilmiştir. Prolin ile muamele edilen tohumlar, stresiz (0 mM NaCl) ve tuz stresli (75 mM NaCl) koşullarda çimlenme testine tabi tutulmuştur. Çimlenme testi sonucunda çimlenme yüzdesi, çimlenme hızı, çimlenme üniformitesi, kökçük uzunluğu ve vigor indeksi değerlendirilmiştir. Elde edilen bulgular, tuz stresinin tüm çimlenme parametreleri üzerinde belirgin bir baskılayıcı etkiye sahip olduğunu ortaya koymuştur. Buna karşın, tohumlara uygulanan prolinin tuz stresinin olumsuz etkilerini önemli ölçüde azalttığı; çimlenme yüzdesi, çimlenme hızı, çimlenme üniformitesi, kökçük uzunluğu ve vigor indeksinde iyileşmeler sağladığı belirlenmiştir. Sonuç olarak, prolin uygulamalarının tuz stresine karşı biber tohumlarının çimlenme performansını artırmada etkili olduğu, özellikle 10 mM ve 15 mM prolin konsantrasyonlarının en başarılı sonuçları verdiği tespit edilmiştir.

Anahtar Kelimeler

• Biber (Capsicum annuum L.)
• priming
• çimlenme
• proline
• stres

Effects of Proline Applications on the Germination of Pepper Seeds under Salt Stress Conditions

Abstract

In this study, the effects of proline applications on the germination characteristics of Capsicum annuum L. (pointed pepper) seeds exposed to salt stress induced by 75 mM NaCl were investigated. In the experiment, four different concentrations of proline [0 (control), 5 mM, 10 mM, and 15 mM] were used, and the seeds were soaked in these solutions for 24 hours. Proline-treated seeds were subjected to germination tests under non-stress (0 mM NaCl) and salt-stress (75 mM NaCl) conditions. As a result of the germination tests, germination percentage, germination rate, germination uniformity, radicle length, and vigor index were evaluated. The findings revealed that salt stress had a pronounced inhibitory effect on all germination parameters. In contrast, proline treatments significantly alleviated the adverse effects of salt stress, leading to improvements in germination percentage, germination rate, germination uniformity, radicle length, and vigor index. In conclusion, proline applications were effective in enhancing the germination performance of pepper seeds under salt stress conditions, with 10 mM and 15 mM proline concentrations yielding the most successful results.

Keywords

• Pepper (Capsicum annuum L.)
• priming
• germination
• proline
• stress

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