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Tuz Stresi Koşullarında Prolin Uygulamalarının Biber Tohumlarının Çimlenmesi Üzerine Etkileri

Yıl 2026, Cilt: 6 Sayı: 1 , 29 - 37 , 30.03.2026
https://izlik.org/JA35MD45SD

Öz

Bu çalışmada, prolin uygulamalarının 75 mM NaCl ile oluşturulan tuz stresi altında Capsicum annuum L. (sivri biber) tohumlarının çimlenme özellikleri üzerine etkileri araştırılmıştır. Denemede, prolinin 0 (kontrol), 5 mM, 10 mM ve 15 mM olmak üzere dört farklı konsantrasyonu kullanılmış ve tohumlar bu solüsyonlarda 24 saat süreyle bekletilmiştir. Prolin ile muamele edilen tohumlar, stresiz (0 mM NaCl) ve tuz stresli (75 mM NaCl) koşullarda çimlenme testine tabi tutulmuştur. Çimlenme testi sonucunda çimlenme yüzdesi, çimlenme hızı, çimlenme üniformitesi, kökçük uzunluğu ve vigor indeksi değerlendirilmiştir. Elde edilen bulgular, tuz stresinin tüm çimlenme parametreleri üzerinde belirgin bir baskılayıcı etkiye sahip olduğunu ortaya koymuştur. Buna karşın, tohumlara uygulanan prolinin tuz stresinin olumsuz etkilerini önemli ölçüde azalttığı; çimlenme yüzdesi, çimlenme hızı, çimlenme üniformitesi, kökçük uzunluğu ve vigor indeksinde iyileşmeler sağladığı belirlenmiştir. Sonuç olarak, prolin uygulamalarının tuz stresine karşı biber tohumlarının çimlenme performansını artırmada etkili olduğu, özellikle 10 mM ve 15 mM prolin konsantrasyonlarının en başarılı sonuçları verdiği tespit edilmiştir.

Kaynakça

  • Ahmadzai, A.S., Hu, C., Zhang, C., & Li, Y. (2025). Mechanisms of anthocyanin-mediated salt stress alleviation and cellular homeostasis in plants. Plant Growth Regulation, 105, 655-673.
  • Aktaş, H., Abak, K., Cakmak, I. (2006). Genotypic variation in the response of pepper to salinity. Scientia Horticulturae, 110(3), 260-266.
  • Ashraf, M., Foolad, M.R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59: 206-216.
  • Bayat, R.A., Kuşvuran, Ş., Ellialtıoğlu, Ş., Üstün, A.S. (2014). Tuz stresi altindaki genç kabak (Cucurbita pepo L. ve C. moschata Poir.) bitkilerine uygulanan prolin’in, antioksidatif enzim aktiviteleri üzerine etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 1(1): 25-33.
  • Chang, B. W., Zhong, P., Liu, J., Tang, Z. H., Gao, Y., Yu, H., & Guo, W. (2019). Effect of low-temperature stress and gibberellin on seed germination and seedling physiological responses in peanut. Acta Agron. Sin, 45(1), 118-130. Chaudhry, S., & Sidhu, G.P.S. (2022). Climate change regulated abiotic stress mechanisms in plants: A comprehensive review. Plant Cell Reports, 41, 1-31.
  • Cha-um, S., Rai, V., & Takabe, T. (2019). Proline, glycinebetaine, and trehalose uptake and inter-organ transport in plants under stress. In Osmoprotectant-mediated abiotic stress tolerance in plants: Recent advances and future perspectives (pp. 201-223). Cham: Springer International Publishing.
  • Dodd, G. L., & Donovan, L. A. (1999). Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. American Journal of Botany, 86(8), 1146-1153.
  • Farooq, M., Basra, S. M., Rehman, H., & Mehmood, T. (2006). Germination and early 49 seedling growth as affected by pre-sowing ethanol seed treatments in fine rice. International Journal of Agriculture and Biology, 8, 19-22.
  • Hosseinifard, M., Stefaniak, S., Ghorbani Javid, M., Soltani, E., Wojtyla, Ł., & Garnczarska, M. (2022). Contribution of exogenous proline to abiotic stresses tolerance in plants: a review. International Journal of Molecular Sciences, 23(9), 5186.
  • Hu, J., Zhu, Z.Y., Song, W.J., Wang, J.C., Hu, W.M. (2005). Effects of sand priming on germination and field performance in direct-sown rice (Oryza sativa L.). Seed Science and Technology 33: 243-248.
  • Hua-long, L., Han-jing, S., Jing-guo, W., Yang, L., De-tang, Z., Hong-wei, Z. (2014). Effect of seed soaking with exogenous proline on seed germination of rice under salt stress. J. Northeast Agric. Univ., 21, 1–6.
  • Karaca, A. (2025a). Effects of Potassium Nitrate Containing Priming on Germination of Tomato (Lycopersicon lycopersicum L.) Seeds Under Salt Stress. Turkish Journal of Agricultural and Natural Sciences, 12(4), 971-978.
  • Karaca, A. (2025b). Effects of cyclic 3-hydroxymelatonin on pepper seed germination under salt and water stress. Bahçe, 54(1), 25-33.
  • Khajeh-Hosseini, M., Powell, A. A., & Bingham, I. J. (2003). The interaction between salinity stress and seed vigour during germination of soyabean seeds. Seed Science and Technology, 31(3), 715-725.
  • Muhammad, M., Waheed, A., Wahab, A., Majeed, M., Nazim, M., Liu, Y.-H., Li, L., & Li, W.J. (2024). Soil salinity and drought tolerance: An evaluation of plant growth, productivity, microbial diversity, and amelioration strategies. Plant Stress, 11, Article 100319
  • Muchate, N. S., Nikalje, G. C., Rajurkar, N. S., Suprasanna, P., & Nikam, T. D. (2016). Plant salt stress: adaptive responses, tolerance mechanism and bioengineering for salt tolerance. The Botanical Review, 82(4), 371-406.
  • Navarro, J.M., Garrido, C., Carvajal, M., Martinez, V. (2002). Yield and fruit quality of pepper plants under sulphate and chloride salinity. The Journal of Horticultural Science and Biotechnology, 77(1), 52-57.
  • Nawaz, K., Talat, A., Hussain, K., & Majeed, A. (2010). Induction of salt tolerance in two cultivars of sorghum (Sorghum bicolor L.) by exogenous application of proline at seedling stage. World Applied Sciences Journal, 10(1), 93-99.
  • Ozbay, N. (2018). Studies on seed priming in pepper (Capsicum annuum L.). In A. Rakshit & H. Singh (Eds.), Advances in seed priming (pp. 209–239). Springer.
  • Perruc, E., Kinoshita, N., & Lopez‐Molina, L. (2007). The role of chromatin‐remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination. The Plant Journal, 52(5), 927-936.
  • Posmyk, M. M., & Janas, K. M. (2007). Effects of seed hydropriming in presence of exogenous proline on chilling injury limitation in Vigna radiata L. seedlings. Acta Physiologiae Plantarum, 29(6), 509-517.
  • Rajjou, L., Duval, M., Gallardo, K., Catusse, J., Bally, J., Job, C., & Job, D. (2012). Seed germination and vigor. Annual Review of Plant Biology, 63, 507-533.
  • Sá, F.V.D.S., Souto, L.S., Paiva, E.P.D., Torres, S. B., & Oliveira, F.A.D. (2019). Initial development and tolerance of pepper species to salinity stress. Revista Caatinga, 32(3), 826-833.
  • Sevgi, B., & Leblebici, S. (2023). Tuz stresinin bitkiler üzerindeki etkileri ve geliştirilen tolerans mekanizmaları. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 11(3), 1498-1516.
  • Singh, M., Singh, A., Nehal, N., Sharma, N. (2018). Effect of proline on germination and seedling growth of rice (Oryza sativa L.) under salt stress. J. Pharmacogn. Phytochem. 7, 2449–2452.
  • Singh, P., Choudhary, K.K., Chaudhary, N., Gupta, S., Sahu, M., Tejaswini, B., & Sarkar, S. (2022). Salt stress resilience in plants mediated through osmolyte accumulation and its crosstalk mechanism with phytohormones. Frontiers in Plant Science, 13, Article 1006617.
  • Subedi, B., Poudel, A., & Aryal, S. (2023). The impact of climate change on insect pest biology and ecology: Implications for pest management strategies, crop production, and food security. Journal of Agriculture and Food Research, 14, 100733.
  • Szabados, L., Savouré, A. (2010). Proline: A multifunctional amino acid. Trends in Plant Science, 15(2): 89-97.
  • Unlukara, A., Demir, I., Kesmez, D., Çelikkol, T., & Demir, K. (2013). Seed yield and quality of pepper plants grown under salt stress. African Journal of Biotechnology, 12(49), 6833-6836.
  • Yaqoob, H., Akram, N. A., Iftikhar, S., Ashraf, M., Khalid, N., Sadiq, M., ... & Ahmad, P. (2019). Seed pretreatment and foliar application of proline regulate morphological, physio-biochemical processes and activity of antioxidant enzymes in plants of two cultivars of quinoa (Chenopodium quinoa Willd.). Plants, 8(12), 588.
  • Yildirim, E., Güvenç, İ. (2006). Salt tolerance of pepper cultivars during germination and seedling growth. Turkish Journal of Agriculture and Forestry, 30(5), 347-353.
  • Yüksel, E. A. (2024). Tuz Stresinin Hafifletilmesinde L-Argininin Zea mays’ da Antioksidan Enzim Aktivitesi Üzerine Etkisi. Turkish Journal of Agriculture-Food Science and Technology, 12(3), 447-452.
  • Zuo, S., Li, J., Gu, W., & Wei, S. (2022). Exogenous proline alleviated low temperature stress in maize embryos by optimizing seed germination, inner proline metabolism, respiratory metabolism and a hormone regulation mechanism. Agriculture, 12(4), 548.
  • Zheng, Y., Cao, D., Zhang, S., Guan, Y. (2008). Effect of polyamines on chilling tolerance in seed imbibition and seed germination in maize. Acta Agronomica Sinica, 34(2), 261–267.

Effects of Proline Applications on the Germination of Pepper Seeds under Salt Stress Conditions

Yıl 2026, Cilt: 6 Sayı: 1 , 29 - 37 , 30.03.2026
https://izlik.org/JA35MD45SD

Öz

In this study, the effects of proline applications on the germination characteristics of Capsicum annuum L. (pointed pepper) seeds exposed to salt stress induced by 75 mM NaCl were investigated. In the experiment, four different concentrations of proline [0 (control), 5 mM, 10 mM, and 15 mM] were used, and the seeds were soaked in these solutions for 24 hours. Proline-treated seeds were subjected to germination tests under non-stress (0 mM NaCl) and salt-stress (75 mM NaCl) conditions. As a result of the germination tests, germination percentage, germination rate, germination uniformity, radicle length, and vigor index were evaluated. The findings revealed that salt stress had a pronounced inhibitory effect on all germination parameters. In contrast, proline treatments significantly alleviated the adverse effects of salt stress, leading to improvements in germination percentage, germination rate, germination uniformity, radicle length, and vigor index. In conclusion, proline applications were effective in enhancing the germination performance of pepper seeds under salt stress conditions, with 10 mM and 15 mM proline concentrations yielding the most successful results.

Kaynakça

  • Ahmadzai, A.S., Hu, C., Zhang, C., & Li, Y. (2025). Mechanisms of anthocyanin-mediated salt stress alleviation and cellular homeostasis in plants. Plant Growth Regulation, 105, 655-673.
  • Aktaş, H., Abak, K., Cakmak, I. (2006). Genotypic variation in the response of pepper to salinity. Scientia Horticulturae, 110(3), 260-266.
  • Ashraf, M., Foolad, M.R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59: 206-216.
  • Bayat, R.A., Kuşvuran, Ş., Ellialtıoğlu, Ş., Üstün, A.S. (2014). Tuz stresi altindaki genç kabak (Cucurbita pepo L. ve C. moschata Poir.) bitkilerine uygulanan prolin’in, antioksidatif enzim aktiviteleri üzerine etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 1(1): 25-33.
  • Chang, B. W., Zhong, P., Liu, J., Tang, Z. H., Gao, Y., Yu, H., & Guo, W. (2019). Effect of low-temperature stress and gibberellin on seed germination and seedling physiological responses in peanut. Acta Agron. Sin, 45(1), 118-130. Chaudhry, S., & Sidhu, G.P.S. (2022). Climate change regulated abiotic stress mechanisms in plants: A comprehensive review. Plant Cell Reports, 41, 1-31.
  • Cha-um, S., Rai, V., & Takabe, T. (2019). Proline, glycinebetaine, and trehalose uptake and inter-organ transport in plants under stress. In Osmoprotectant-mediated abiotic stress tolerance in plants: Recent advances and future perspectives (pp. 201-223). Cham: Springer International Publishing.
  • Dodd, G. L., & Donovan, L. A. (1999). Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. American Journal of Botany, 86(8), 1146-1153.
  • Farooq, M., Basra, S. M., Rehman, H., & Mehmood, T. (2006). Germination and early 49 seedling growth as affected by pre-sowing ethanol seed treatments in fine rice. International Journal of Agriculture and Biology, 8, 19-22.
  • Hosseinifard, M., Stefaniak, S., Ghorbani Javid, M., Soltani, E., Wojtyla, Ł., & Garnczarska, M. (2022). Contribution of exogenous proline to abiotic stresses tolerance in plants: a review. International Journal of Molecular Sciences, 23(9), 5186.
  • Hu, J., Zhu, Z.Y., Song, W.J., Wang, J.C., Hu, W.M. (2005). Effects of sand priming on germination and field performance in direct-sown rice (Oryza sativa L.). Seed Science and Technology 33: 243-248.
  • Hua-long, L., Han-jing, S., Jing-guo, W., Yang, L., De-tang, Z., Hong-wei, Z. (2014). Effect of seed soaking with exogenous proline on seed germination of rice under salt stress. J. Northeast Agric. Univ., 21, 1–6.
  • Karaca, A. (2025a). Effects of Potassium Nitrate Containing Priming on Germination of Tomato (Lycopersicon lycopersicum L.) Seeds Under Salt Stress. Turkish Journal of Agricultural and Natural Sciences, 12(4), 971-978.
  • Karaca, A. (2025b). Effects of cyclic 3-hydroxymelatonin on pepper seed germination under salt and water stress. Bahçe, 54(1), 25-33.
  • Khajeh-Hosseini, M., Powell, A. A., & Bingham, I. J. (2003). The interaction between salinity stress and seed vigour during germination of soyabean seeds. Seed Science and Technology, 31(3), 715-725.
  • Muhammad, M., Waheed, A., Wahab, A., Majeed, M., Nazim, M., Liu, Y.-H., Li, L., & Li, W.J. (2024). Soil salinity and drought tolerance: An evaluation of plant growth, productivity, microbial diversity, and amelioration strategies. Plant Stress, 11, Article 100319
  • Muchate, N. S., Nikalje, G. C., Rajurkar, N. S., Suprasanna, P., & Nikam, T. D. (2016). Plant salt stress: adaptive responses, tolerance mechanism and bioengineering for salt tolerance. The Botanical Review, 82(4), 371-406.
  • Navarro, J.M., Garrido, C., Carvajal, M., Martinez, V. (2002). Yield and fruit quality of pepper plants under sulphate and chloride salinity. The Journal of Horticultural Science and Biotechnology, 77(1), 52-57.
  • Nawaz, K., Talat, A., Hussain, K., & Majeed, A. (2010). Induction of salt tolerance in two cultivars of sorghum (Sorghum bicolor L.) by exogenous application of proline at seedling stage. World Applied Sciences Journal, 10(1), 93-99.
  • Ozbay, N. (2018). Studies on seed priming in pepper (Capsicum annuum L.). In A. Rakshit & H. Singh (Eds.), Advances in seed priming (pp. 209–239). Springer.
  • Perruc, E., Kinoshita, N., & Lopez‐Molina, L. (2007). The role of chromatin‐remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination. The Plant Journal, 52(5), 927-936.
  • Posmyk, M. M., & Janas, K. M. (2007). Effects of seed hydropriming in presence of exogenous proline on chilling injury limitation in Vigna radiata L. seedlings. Acta Physiologiae Plantarum, 29(6), 509-517.
  • Rajjou, L., Duval, M., Gallardo, K., Catusse, J., Bally, J., Job, C., & Job, D. (2012). Seed germination and vigor. Annual Review of Plant Biology, 63, 507-533.
  • Sá, F.V.D.S., Souto, L.S., Paiva, E.P.D., Torres, S. B., & Oliveira, F.A.D. (2019). Initial development and tolerance of pepper species to salinity stress. Revista Caatinga, 32(3), 826-833.
  • Sevgi, B., & Leblebici, S. (2023). Tuz stresinin bitkiler üzerindeki etkileri ve geliştirilen tolerans mekanizmaları. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 11(3), 1498-1516.
  • Singh, M., Singh, A., Nehal, N., Sharma, N. (2018). Effect of proline on germination and seedling growth of rice (Oryza sativa L.) under salt stress. J. Pharmacogn. Phytochem. 7, 2449–2452.
  • Singh, P., Choudhary, K.K., Chaudhary, N., Gupta, S., Sahu, M., Tejaswini, B., & Sarkar, S. (2022). Salt stress resilience in plants mediated through osmolyte accumulation and its crosstalk mechanism with phytohormones. Frontiers in Plant Science, 13, Article 1006617.
  • Subedi, B., Poudel, A., & Aryal, S. (2023). The impact of climate change on insect pest biology and ecology: Implications for pest management strategies, crop production, and food security. Journal of Agriculture and Food Research, 14, 100733.
  • Szabados, L., Savouré, A. (2010). Proline: A multifunctional amino acid. Trends in Plant Science, 15(2): 89-97.
  • Unlukara, A., Demir, I., Kesmez, D., Çelikkol, T., & Demir, K. (2013). Seed yield and quality of pepper plants grown under salt stress. African Journal of Biotechnology, 12(49), 6833-6836.
  • Yaqoob, H., Akram, N. A., Iftikhar, S., Ashraf, M., Khalid, N., Sadiq, M., ... & Ahmad, P. (2019). Seed pretreatment and foliar application of proline regulate morphological, physio-biochemical processes and activity of antioxidant enzymes in plants of two cultivars of quinoa (Chenopodium quinoa Willd.). Plants, 8(12), 588.
  • Yildirim, E., Güvenç, İ. (2006). Salt tolerance of pepper cultivars during germination and seedling growth. Turkish Journal of Agriculture and Forestry, 30(5), 347-353.
  • Yüksel, E. A. (2024). Tuz Stresinin Hafifletilmesinde L-Argininin Zea mays’ da Antioksidan Enzim Aktivitesi Üzerine Etkisi. Turkish Journal of Agriculture-Food Science and Technology, 12(3), 447-452.
  • Zuo, S., Li, J., Gu, W., & Wei, S. (2022). Exogenous proline alleviated low temperature stress in maize embryos by optimizing seed germination, inner proline metabolism, respiratory metabolism and a hormone regulation mechanism. Agriculture, 12(4), 548.
  • Zheng, Y., Cao, D., Zhang, S., Guan, Y. (2008). Effect of polyamines on chilling tolerance in seed imbibition and seed germination in maize. Acta Agronomica Sinica, 34(2), 261–267.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sebze Yetiştirme ve Islahı
Bölüm Araştırma Makalesi
Yazarlar

Nusret Özbay 0000-0001-9642-119X

Aygül Karaca 0000-0001-9142-9678

Gönderilme Tarihi 20 Şubat 2026
Kabul Tarihi 18 Mart 2026
Yayımlanma Tarihi 30 Mart 2026
IZ https://izlik.org/JA35MD45SD
Yayımlandığı Sayı Yıl 2026 Cilt: 6 Sayı: 1

Kaynak Göster

APA Özbay, N., & Karaca, A. (2026). Effects of Proline Applications on the Germination of Pepper Seeds under Salt Stress Conditions. Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi, 6(1), 29-37. https://izlik.org/JA35MD45SD