Exosome isolation by ultracentrifugation and precipitation methods from human adipose-derived mesenchymal stem cells and the effects on human colon cancer cell line.

Abstract

Exosomes, one of the extracellular vesicles, mediate molecular information and intercellular communication between cells by containing various proteins, lipids, mRNA, metabolic enzymes and numerous essential molecules such as miRNAs. The therapeutic potential of paracrine factors of exosomes released from human adipose mesenchymal stem cells is still under investigation. In our study, we isolated exosomes released from human adipose mesenchymal stem cells by two different methods. To evaluate the cytotoxic effect of the obtained exosomes, we performed MTT assay in SW-480 cell line at 24, 48 and 72 hours. In the results obtained, no statistically significant difference was observed at 24 and 72 hours, while statistically significant differences were determined between the concentration groups at 48 hours.

Keywords

• MTT
• exosome
• colon cancer
• mesenchymal stem cells

İnsan yağ dokusundan elde edilen mezenkimal kök hücrelerinden ultra santrifüj ve çöktürme yöntemleriyle ekzosom izolasyonu ve insan kolon kanseri hücre hattına etkileri.

Abstract

Hücre dışı veziküllerden biri olan ekzosomlar, çeşitli proteinler, lipitler, mRNA, metabolik enzimler ve miRNA gibi çok sayıda esansiyel molekülü içererek hücreler arasındaki moleküler bilgiyi ve hücreler arası iletişimi sağlar. İnsan yağ mezenkimal kök hücrelerinden salınan ekzosomların parakrin faktörlerinin terapötik potansiyeli hala araştırılmaktadır. Çalışmamızda, insan yağ mezenkimal kök hücrelerinden salınan ekzosomlar iki farklı yöntemle izole edildi. Elde edilen ekzosomların sitotoksik etkisini değerlendirmek için SW-480 hücre hattında 24, 48 ve 72. saatlerde MTT analizi yapıldı. Elde edilen sonuçlarda, 24 ve 72. saatlerde istatistiksel olarak anlamlı bir fark gözlenmezken, 48. saatte konsantrasyon grupları arasında istatistiksel olarak anlamlı farklar belirlendi.

Keywords

• MTT
• kolon kanseri
• eksozom
• mezenkimal kök hücre

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