Transposon studies on Colchium chalcedonicum

Year 2019, , 25 - 35, 21.04.2019

Elif Karlık , Merve Albayrak Erdal Uzen , Nermin Gözükırmızı

https://doi.org/10.38001/ijlsb.529237

Abstract

Colchicum chalcedonicum is one of
the endemic plants in Turkey. The aim of this study was the investigation of
the retrotransposon SIRE1, Sukkula
and Nikita presence and insertion
patterns in C. chalcedonicum. The
plant samples were collected from the botanic garden of the Istanbul
University. DNA isolation was performed from leaves by using modified
CTAB/SEVAG protocol. Retrotransposon movements were investigated using SIRE1, Sukkula and Nikita primers by Inter Retrotranposon Amplified Polymorphism PCR
technique (IRAP-PCR). Polymorphism percentages (%) were calculated based on
Jaccard Similarity Index. We observed that polymorphism ratios of SIRE1, Sukkula and Nikita
retrotransposons among all samples were 0-40%, 0-100% and 0-60%, respectively.
This is the first report to demonstrate three barley ─ SIRE1, Sukkula and Nikita─ retrotransposons presence and
movements in C. chalcedonicum which
is belonged to Colchium family, thus these IRAP primers may be
used in further characterization and diversity studies of Colchicum
family. 

 

Keywords

Colchicum chalcedonicum, Retrotransposons, SIRE1, Sukkula, Nikita

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