Isolation of bacteria from Tuz Gölü lake that can grow on high salt concentration

Abstract

Today, extremophile is widely studied by the scientist due to its strong survival features that allow them to survive under extreme environment. Halophile is one example that inhabit high salt concentration environment. Isolation of bacteria from the area of Tuz Gölü lake, also known as hypersaline lake in the central plateau of Turkey, led to the isolation of 4 halotolerant bacteria, which were able to grow optimally in media with 0–10% of salt. Surprisingly, the strain A-4 isolate was successfully isolated from the Tuz Gölü lake water on the minimal media that consists of 2,2-dichloropropionic acid as a carbon source. This indicated that the strain A-4 was very useful in the environmental remediation due to its capability to break down 2,2-DCP, a halocarboxylic found in herbicide.  Further analysis such as biochemical tests and 16srRNA sequence analysis were necessary to further identify the species of the bacteria in the next experiment.

Keywords

• Halophile,halotolerant bacteria,tuz gölu

References

1. Camur, M. Z., and Mutlu, H., Major-ion geochemistry and mineralogy of the Salt Lake (Tuz Gölü) basin, Turkey. Chemical Geology, 1996.127(4): p. 313-329.
2. Dumorné, K., Córdova, D. C., Astorga-Eló, M., and Renganathan, P., Extremozymes: a potential source for industrial applications. Journal Microbiol Biotechnol, 2017. 27(4): p.649-659.
3. Edbeib, M. F., Wahab, R. A., Kaya, Y., and Huyop, F., In silico characterization of a novel dehalogenase (DehHX) from the halophile Pseudomonas halophila HX isolated from Tuz Gölü Lake, Turkey: insights into a hypersaline-adapted dehalogenase. Annals of Microbiology, 2017. 67(5): p. 371-382.
4. Hareland, W. A., Crawford, R. L., Chapman, P. J., and Dagley, S., Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. Journal of Bacteriology, 1975. 121(1), 272-285.
5. Huston, A. L., Krieger-Brockett, B. B., & Deming, J. W., Remarkably low temperature optima for extracellular enzyme activity from Arctic bacteria and sea ice. Environmental microbiology, 2000. 2(4), 383-388.
6. Ma, Y., Galinski, E. A., Grant, W. D., Oren, A., and Ventosa, A., Halophiles 2010: life in saline environments. In: Am Soc Microbiol., 2010.
7. Mir, S. I., Gasim, M. B., Rahim, S. A., and Toriman, M. E., Soil loss assessment in the Tasik Chini catchment, Pahang, Malaysia., 2010.
8. Mutlu, M. B., Martínez-García, M., Santos, F., Peña, A., Guven, K., & Antón, J., Prokaryotic diversity in Tuz Lake, a hypersaline environment in Inland Turkey. FEMS microbiology ecology, 2008. 65(3), 474-483

9. Ollivier, B., Caumette, P., Garcia, J.-L., & Mah, R., Anaerobic bacteria from hypersaline environments. Microbiology and Molecular Biology Reviews, 1994. 58(1), 27-38.
10. Paul, V. G., & Mormile, M. R., A case for the protection of saline and hypersaline environments: a microbiological perspective. FEMS microbiology ecology,2017. 93(8).
11. Sorokin, D. Y., Tourova, T., Galinski, E., Belloch, C., & Tindall, B., Extremely halophilic denitrifying bacteria from hypersaline inland lakes, Halovibrio denitrificans sp. nov. and Halospina denitrificans gen. nov., sp. nov., and evidence that the genus name Halovibrio Fendrich 1989 with the type species Halovibrio variabilis should be associated with DSM 3050. International journal of systematic and evolutionary microbiology, 2006.56(2), 379-388.