Improvement of Bioavailability of Sage and Mint by Ultrasonic Extraction

Abstract

Plant extracts are complex mixtures obtained from fruites, leaves, flowers, woods, resins and seeds of a fresh or dried plant by various methods. Because of the disadvantages of conventional extraction methods such as ultrasound assisted systems have drawn higher attention in recent years.

In this study, the ultrasound-assisted extraction(USE) conditions(temperature, time, ultrasonication power and solvent-solid ratio) were determined for the production of crude extracts from sage (Salvia officinalis) and mint (Mentha piperita). Bioavailability of the plant extracts were also compared to those obtained by classical hot-water extraction (HWE).  USE parameters for the highest yield were 40 ⁰C, 10 min, 400 W, as determined by preliminary experiements. Total peholic contents of the mint and sage samples increased by ultrasound assisted liquid extraction method at the levels of 23.88 % and 14.97 %, respectively. The bioavailability of total phenolic contents in classis and ultrasound extraction of mint (Mentha piperita) extract was 20.11 %, 32.45 % respectively. In conclusion, the results of the present study showed that ultrasound assisted extraction was more effective method for extraction of bioactive substances from sage and mint with shorther extraction time, increased bioactivity and bioavailability.

Keywords

• Medicinal herbs extract,ultrasound-assisted extraction,Bioavailability

Ultrasonik Ekstraksiyon ile Adaçayı ve Nanenin Biyoyararlılığının Geliştirilmesi

Abstract

Bitkisel ekstraktlar, taze veya kurutulmuş bir bitkinin meyvelerinden, yapraklarından, çiçeklerinden, odunlarından, reçinelerinden ve çekirdeklerinden çeşitli yöntemlerle elde edilen karmaşık karışımlardır. Geleneksel ekstraksiyon uygulamaları çok uzun zaman almakta ve büyük miktarlarda solvente ihtiyaç duyulmaktadır. Ultrason destekli sistemlerin ekstraksiyonda kullanımı konusundaki çalışmalar son yıllarda giderek artmaktadır.

 

Bu çalışmada, adaçayı (Salvia officinalis) ve nane (Mentha piperita) 'dan elde edilen ham özütlerin üretimi için ultrason destekli ekstraksiyon (USE) koşulları (sıcaklık, zaman, ultrason gücü ve çözücü-katı oranı) belirlenmiştir. Bitki ekstrelerinin biyoyararlanımı ayrıca klasik sıcak su ekstraksiyonu (HWE) ile elde edilenlerle karşılaştırıldı. Ön denemelerle belirlenen en yüksek ekstraksiyon veriminin elde edildiği paremetreler 40 ° C, 10 dak, 400 W kullanılmıştır. Nane numunelerinin toplam fenolik içeriği, ultrason destekli sıvı ekstraksiyon yöntemiyle% 23.88 oranında artarken, adaçayı numuneleri% 14.97 arttı. Nane (Mentha Piperita) ekstrelerinin klasik ve ultrason ekstraksiyonunda toplam fenolik içeriğin biyoyararlanımı sırasıyla % 20.11,% 32.45 idi.

 

Bu çalışmanın sonuçları gösteriyor ki, ultrason destekli ekstraksiyon, tıbbi bitkilerden biyoaktif maddelerin elde edilmesinde klasik yönteme göre daha verimli olmuştur. Biyoaktif madde miktarını arttırırken, biyoyararlılıklarını  da arttırmıştır.

Keywords

• ultrason destekli ekstraksiyon,Tıbbi bitki ekstraktı,biyoyararlılık

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