Salvia fruticosa’nın (Anadolu Adaçayı) Terapötik Etkileri

Abstract

Anadolu adaçayı (Salvia fruticosa), Lamiaceae familyasına ait bir bitki olup, Anadolu’da geleneksel halk hekimliğinde yaygın olarak kullanılmaktadır. Bitkinin yaprakları çay şeklinde tüketilir ve yapraklarından uçucu yağ elde edilir. Salvia fruticosa bitkisi farmasötik ajan olarak (antioksidan, antikanser, antimikrobiyal, antifungal, antihiperglisemik, antihipertansif vb.) kullanımı dışında aromaterapi, parfümeri ve kozmetik sektöründe; yiyeceklerde baharat, aroma verici ve koruyucu olarak pek çok alanda kullanıma sahiptir. Ancak bitkinin irrasyonel kullanımı ciddi yan etkilere ve komplikasyonlara yol açmaktadır. Bu derleme, Salvia fruticosa’nın botanik özelliklerini, uçucu yağ bileşenlerini, bu bileşenlerin potansiyel terapötik etkilerini ve neden olabileceği olası toksik etkileri değerlendirmeyi amaçlamaktadır.

Keywords

• Anadolu adaçayı
• Salvia fruticosa
• Terapötik etkiler
• Toksik etkiler

References

1. Referans1 Tundis, R., et al., Assessment of antioxidant, antitumor and pro-apoptotic effects of Salvia fruticosa Mill. subsp. thomasii (Lacaita) Brullo, Guglielmo, Pavone & Terrasi (Lamiaceae). Food and Chemical Toxicology, 2017. 106: p. 155-164.
2. Referans2 Essawi, T. and M. Srour, Screening of some Palestinian medicinal plants for antibacterial activity. Journal of ethnopharmacology, 2000. 70(3): p. 343-349.
3. Referans3 Boukhary, R., et al., Anti-inflammatory and antioxidant activities of Salvia fruticosa: An HPLC determination of phenolic contents. Evidence-Based Complementary and Alternative Medicine, 2016. 2016.
4. Referans4 Lu, Y. and L.Y. Foo, Polyphenolics of Salvia—a review. Phytochemistry, 2002. 59(2): p. 117-140.
5. Referans5 Topçu, G., Bioactive triterpenoids from Salvia species. Journal of natural products, 2006. 69(3): p. 482-487.
6. Referans6 Gürdal, B. and Ş. Kültür, An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). Journal of Ethnopharmacology, 2013. 146(1): p. 113-126.
7. Referans7 WCSP, World checklist of selected plant families. Facilitated by the Royal Botanic Gardens, Kew, 2014.
8. Referans8 Gali-Muhtasib, H., Anticancer and medicinal properties of essential oil and extracts of East Mediterranean sage (Salvia triloba). Advances in Phytomedicine, 2006. 2: p. 169-180.

9. Referans9 Dincer, C., et al., A comparative study on phenolic composition, antioxidant activity and essential oil content of wild and cultivated sage (Salvia fruticosa Miller) as influenced by storage. Industrial Crops and Products, 2012. 39: p. 170-176.
10. Referans10 Şenol, F.S., et al., Survey of 55 Turkish Salvia taxa for their acetylcholinesterase inhibitory and antioxidant activities. Food Chemistry, 2010. 120(1): p. 34-43.
11. Referans11 Atmaca, H. and E. Bozkurt, Apoptotic and anti-angiogenic effects of Salvia triloba extract in prostate cancer cell lines. Tumor Biology, 2016. 37(3): p. 3639-3646.
12. Referans12 Delamare, A.P.L., et al., Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food chemistry, 2007. 100(2): p. 603-608.
13. Referans13 Zihlif, M., et al., The antiangiogenic activities of ethanolic crude extracts of four Salvia species. BMC Complementary and Alternative Medicine, 2013. 13(1): p. 358.
14. Referans14 Exarchou, V., et al., HPLC-SPE-NMR characterization of major metabolites in Salvia fruticosa Mill. Extract with antifungal potential: Relevance of carnosic acid, carnosol, and hispidulin. Journal of agricultural and food chemistry, 2015. 63(2): p. 457-463.
15. Referans15 Azevedo, M.F., et al., Rosmarinic acid, major phenolic constituent of Greek sage herbal tea, modulates rat intestinal SGLT1 levels with effects on blood glucose. Molecular nutrition & food research, 2011. 55(S1): p. S15-S25.
16. Referans16 Xavier, C.P., et al., Salvia fruticosa, Salvia officinalis, and rosmarinic acid induce apoptosis and inhibit proliferation of human colorectal cell lines: the role in MAPK/ERK pathway. Nutrition and cancer, 2009. 61(4): p. 564-571.
17. Referans17 TUBIVES, Turkish Plants Data Service. 2004. Available from: http://www.tubives.com/ [Erişim Tarihi: 29.04.2020].
18. Referans18 Bayram, E. and Ç. Sönmez, Adaçayı Yetiştiriciliği. EÜ Tar. Uyg. ve Araş. Merkezi Yayım Bülteni, 2006(48).
19. Referans19 Papageorgiou, V., et al., Variation of the chemical profile and antioxidant behavior of Rosmarinus officinalis L. and Salvia fruticosa Miller grown in Greece. Journal of agricultural and food chemistry, 2008. 56(16): p. 7254-7264.
20. Referans20 Ceylan A., Ege University Agriculture Faculty Press, Izmir, Turkey, p. 225 (1997).
21. Referans21 Putievsky, E., U. Ravid, and N. Dudai, The essential oil and yield components from various plant parts of Salvia fruticosa. Journal of natural products, 1986. 49(6): p. 1015-1017.
22. Referans22 Altay, A., et al., Anatolian sage Salvia fruticosa inhibits cytosolic glutathione-s-transferase activity and colon cancer cell proliferation. Journal of Food Measurement and Characterization, 2019. 13(2): p. 1390-1399.
23. Referans23 Bayram, E., A. Ceylan, and H. Geren, Anadolu adaçayı (Salvia fruticosa Mill.) ıslahında geliştirilen klonların agronomik ve kalite özellikleri üzerinde araştırma. Türkiye, 1999. 3: p. 212-217.
24. Referans24 Zgheib, R., et al., Investigation of essential oil chemical polymorphism of Salvia fruticosa naturally growing in Lebanon. Journal of Essential Oil Bearing Plants, 2019. 22(2): p. 408-430.
25. Referans25 Karioti, A., et al., Effect of nitrogen concentration of the nutrient solution on the volatile constituents of leaves of Salvia fruticosa Mill. in solution culture. Journal of agricultural and food chemistry, 2003. 51(22): p. 6505-6508.
26. Referans26 Sarrou, E., S. Martens, and P. Chatzopoulou, Metabolite profiling and antioxidative activity of Sage (Salvia fruticosa Mill.) under the influence of genotype and harvesting period. Industrial Crops and Products, 2016. 94: p. 240-250.
27. Referans27 Bellomaria, B., et al., Contribution to the study of the essential oils from three species of Salvia growing wild in the eastern Mediterranean region. Journal of Essential Oil Research, 1992. 4(6): p. 60.
28. Referans28 El-Wahab, A., et al., Effect of different planting locations in Egypt on Salvia fruticosa mill. Plants. Egyptian Journal of Desert Research, 2015. 65(2): p. 291-307.
29. Referans29 Altindal, D., Determination of genetic diversity of natural sage populations in Muğla region of Turkey. International Journal of Environmental Science and Technology, 2019. 16(9): p. 5219-5226.
30. Referans30 Karousou, R., D. Vokou, and S. Kokkini, Variation of Salvia fruticosa essential oils on the island of Crete (Greece). Botanica acta, 1998. 111(3): p. 250-254.
31. Referans31 Skoula, M., J.E. Abbes, and C.B. Johnson, Genetic variation of volatiles and rosmarinic acid in populations of Salvia fruticosa mill growing in Crete. Biochemical systematics and ecology, 2000. 28(6): p. 551-561.
32. Referans32 Pitarokili, D., et al., Volatile metabolites from Salvia fruticosa as antifungal agents in soilborne pathogens. Journal of agricultural and food chemistry, 2003. 51(11): p. 3294-3301.
33. Referans33 Kaplan, M., et al. The effects of different organic manure applications on the dry weight and the essential oil quantity of sage (Salvia fruticosa Mill.). in I International Medicinal and Aromatic Plants Conference on Culinary Herbs 826. 2007.
34. Referans34 Kocabas, I., et al., Effects of different organic manure applications on the essential oil components of Turkish sage (Salvia fruticosa Mill.). Asian Journal of chemistry, 2010. 22(2): p. 1599.
35. Referans35 Uritu, C.M., et al., Medicinal plants of the family Lamiaceae in pain therapy: A review. Pain Research and Management, 2018. 2018.
36. Referans36 Alzoreky, N. and K. Nakahara, Antibacterial activity of extracts from some edible plants commonly consumed in Asia. International journal of food microbiology, 2003. 80(3): p. 223-230.
37. Referans37 Sivropoulou, A., et al., Antimicrobial, cytotoxic, and antiviral activities of Salvia fructicosa essential oil. Journal of Agricultural and food Chemistry, 1997. 45(8): p. 3197-3201.
38. Referans38 Aşkun, T., et al., Characterization of essential oils of some Salvia species and their antimycobacterial activities. Turkish Journal of Biology, 2010. 34(1): p. 89-95.
39. Referans39 Duletić-Laušević, S., et al., Composition and biological activities of Libyan Salvia fruticosa Mill. and S. lanigera Poir. extracts. South African Journal of Botany, 2018. 117: p. 101-109.
40. Referans40 Giweli, A., et al., Antimicrobial and antioxidant activities of essential oils of Satureja thymbra growing wild in Libya. Molecules, 2012. 17(5): p. 4836-4850.
41. Referans41 Karadağ, A.E., et al., Antibacterial evaluation of Elettaria cardamomum (L.) Maton, Lavandula angustifolia Mill. and Salvia fruticosa Mill. essential oil combinations in mouthwash preparations. Natural Volatiles&Essential Oils, 2020. 7(1): p. 9-17.
42. Referans42 Soković, M. and L.J. Van Griensven, Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of plant pathology, 2006. 116(3): p. 211-224.
43. Referans43 Ağırman, B., M.N. Akalın, and H. Erten, Meyve ve sebzelerde hasat sonrası fungal hastalıkların antagonistik mayalar ile biyokontrolü. Gıda, 2019. 44(1): p. 31-49.
44. Referans44 Dwivedi, S.K. and N. Dubey, Potential use of the essential oil of Trachyspermum ammi against seed-borne fungi of Guar (Cyamopsis tetragonoloba L.(Taub.) Mycopathologia, 1993. 121(2): p. 101-104.
45. Referans45 Mishra, A. and N. Dubey, Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and environmental microbiology, 1994. 60(4): p. 1101-1105.
46. Referans46 Kadoglidou, K., et al., Inhibitory and stimulatory effects of essential oils and individual monoterpenoids on growth and sporulation of four soil-borne fungal isolates of Aspergillus terreus, Fusarium oxysporum, Penicillium expansum, and Verticillium dahliae. European journal of plant pathology, 2011. 130(3): p. 297-309.
47. Referans47 Abou-Jawdah, Y., H. Sobh, and A. Salameh, Antimycotic activities of selected plant flora, growing wild in Lebanon, against phytopathogenic fungi. Journal of agricultural and food chemistry, 2002. 50(11): p. 3208-3213.
48. Referans48 Shimoni, M., et al., Antifungal activity of volatile fractions of essential oils from four aromatic wild plants in Israel. Journal of chemical ecology, 1993. 19(6): p. 1129-1133.
49. Referans49 Adam, K., et al., Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. Journal of Agricultural and Food Chemistry, 1998. 46(5): p. 1739-1745.
50. Referans50 Ali‐Shtayeh, M. and S.I. Abu Ghdeib, Antifungal activity of plant extracts against dermatophytes. mycoses, 1999. 42(11‐12): p. 665-672.
51. Referans51 Urek, R.O., et al., Comparison of antioxidant capacities of the leaves and flowers of Salvia fruticosa grown in Turkey. Asian Journal of Chemistry, 2008. 20(3): p. 2091.
52. Referans52 Valko, M., et al., Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions, 2006. 160(1): p. 1-40.
53. Referans53 Hani, S.B. and M. Bayachou, Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry. Asian Pacific Journal of Tropical Biomedicine, 2014. 4(5): p. 399-403.
54. Referans54 Garg, D., et al., In-vitro antioxidant activity and phytochemical analysis in extracts of Hibiscus rosa-sinensis stem and leaves. Free Radicals and Antioxidants, 2012. 2(3): p. 41-46.
55. Referans55 Rice-Evans, C., N. Miller, and G. Paganga, Antioxidant properties of phenolic compounds. Trends in plant science, 1997. 2(4): p. 152-159.
56. Referans56 Özyürek, M., Reaktif Oksijen Türleri Süpürücü Antioksidan Aktivitenin Ölçümünde Modifiye CUPRAC Yöntemlerinin Geliştirilmesi. İstanbul Üniversitesi, Doktora tezi, İstanbul, 2008.
57. Referans57 Pizzale, L., et al., Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanum onites and O indercedens) extracts related to their phenolic compound content. Journal of the Science of Food and Agriculture, 2002. 82(14): p. 1645-1651.
58. Referans58 Bozan, B., et al., Antioxidant and free radical scavenging activities of eight Salvia species. Chemistry of Natural Compounds, 2002. 38(2): p. 198-200.
59. Referans59 Pasias, I.N., et al., Elemental content and total antioxidant activity of Salvia fruticosa. Food analytical methods, 2010. 3(3): p. 195-204.
60. Referans60 Giweli, A.A., et al., The chemical composition, antimicrobial and antioxidant activities of the essential oil of Salvia fruticosa growing wild in Libya. Archives of Biological Sciences, 2013. 65(1): p. 321-329.
61. Referans61 Durling, N.E., et al., Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures. Food chemistry, 2007. 101(4): p. 1417-1424.
62. Referans62 Hoeijmakers, J.H., DNA damage, aging, and cancer. New England Journal of Medicine, 2009. 361(15): p. 1475-1485.
63. Referans63 Wild, C., E. Weiderpass, and B. Stewart, World cancer report: cancer research for cancer prevention. Lyon: International Agency for Research on Cancer, 2020.
64. Referans64 Yıllığı, S.İ., TC Sağlık Bakanlığı. Sağlık Araştırmaları Genel Müdürlüğü. Ankara, 2017.
65. Referans65 Gali-Muhtasib, H. and N. Bakkar, Modulating cell cycle: current applications and prospects for future drug development. Current cancer drug targets, 2002. 2(4): p. 309-336.
66. Referans66 Abu-Dahab, R., et al., Mechanistic studies of antiproliferative effects of Salvia triloba and Salvia dominica (Lamiaceae) on breast cancer cell lines (MCF7 and T47D). Zeitschrift für Naturforschung C, 2014. 69(11-12): p. 443-451.
67. Referans67 Shih, H., G.V. Pickwell, and L.C. Quattrochi, Differential effects of flavonoid compounds on tumor promoter-induced activation of the human CYP1A2 enhancer. Archives of Biochemistry and Biophysics, 2000. 373(1): p. 287-294.
68. Referans68 Altay, A. and F. Bozoğlu, Salvia fruticosa modulates mRNA expressions and activity levels of xenobiotic metabolizing CYP1A2, CYP2E1, NQO1, GPx, and GST enzymes in human colorectal adenocarcinoma HT-29 cells. Nutrition and cancer, 2017. 69(6): p. 892-903.
69. Referans 69 Akaberi, M., S. Mehri, and M. Iranshahi, Multiple pro-apoptotic targets of abietane diterpenoids from Salvia species. Fitoterapia, 2015. 100: p. 118-132.
70. Referans70 Gali-Muhtasib, H. and N. Affara, Chemopreventive effects of sage oil on skin papillomas in mice. Phytomedicine, 2000. 7(2): p. 129-136.
71. Referans71 Kaileh, M., et al., Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. Journal of ethnopharmacology, 2007. 113(3): p. 510-516.
72. Referans72 Badisa, R., et al., Cytotoxic Activities of Salvia of the Labiatae Family. Pharmaceutical biology, 2005. 42(8): p. 640-645.
73. Referans73 Agarwal, R., Cell signaling and regulators of cell cycle as molecular targets for prostate cancer prevention by dietary agents. Biochemical pharmacology, 2000. 60(8): p. 1051-1059.
74. Referans74 Kan, S.F., et al., Anti‐proliferative effects of evodiamine on human prostate cancer cell lines DU145 and PC3. Journal of cellular biochemistry, 2007. 101(1): p. 44-56.
75. Referans75 Ramos, A.A., et al., Polyphenolic compounds from Salvia species protect cellular DNA from oxidation and stimulate DNA repair in cultured human cells. Journal of agricultural and food chemistry, 2010. 58(12): p. 7465-7471.
76. Referans76 Al-Kalaldeh, J.Z., R. Abu-Dahab, and F.U. Afifi, Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutrition Research, 2010. 30(4): p. 271-278.
77. Referans77 Abu-Dahab, R., et al., Comparison of the antiproliferative activity of crude ethanol extracts of nine salvia species grown in Jordan against breast cancer cell line models. Pharmacognosy magazine, 2012. 8(32): p. 319.
78. Referans78 Sevindik, N. and E. Rencuzogullari, The genotoxic and antigenotoxic effects of Salvia fruticosa leaf extract in human blood lymphocytes. Drug and chemical toxicology, 2014. 37(3): p. 295-302.
79. Referans79 Fraihat, A., et al., Inhibitory effects of methanol extracts of selected plants on the proliferation of two human melanoma cell lines. Tropical Journal of Pharmaceutical Research, 2018. 17(6): p. 1081-1086.
80. Referans80 Koutsoulas, A., et al., Characterization of phenolic compounds and antiproliferative effects of Salvia pomifera and Salvia fruticosa extracts. Molecules, 2019. 24(16): p. 2921.
81. Referans81 Eltawaty, S.I., et al., Anticancer effects of methanol extract of Libyan Salvia fruticosa Mill. on MCF7, T47D and (MDA-MB-468) breast cells lines. European Journal of Pharmaceutical and Medical Research, 7(2): p. 165-169.
82. Referans82 Folkman, S. and R.S. Lazarus, Stress, appraisal, and coping. 1984: New York: Springer Publishing Company.
83. Referans83 Hur, J.M., et al., Cryptotanshinone but not tanshinone IIA inhibits angiogenesis in vitro. Experimental & molecular medicine, 2005. 37(2): p. 133-137.
84. Referans84 Jung, H.-J., et al., Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of an ethanol extract of Salvia plebeia R. Brown. Journal of ethnopharmacology, 2009. 126(2): p. 355-360.
85. Referans85 Keshavarz, M., et al., In vitro and ex vivo antiangiogenic activity of Salvia officinalis. Phytotherapy Research, 2010. 24(10): p. 1526-1531.
86. Referans86 Liu, F., et al., Effect of Salvia chinensis extraction on angiogenesis of tumor. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica, 2012. 37(9): p. 1285-1288.
87. Referans87 Perry, N.S., et al., In‐vitro inhibition of human erythrocyte acetylcholinesterase by Salvia lavandulaefolia essential oil and constituent terpenes. Journal of pharmacy and pharmacology, 2000. 52(7): p. 895-902.
88. Referans88 Savelev, S.U., E.J. Okello, and E.K. Perry, Butyryl‐and acetyl‐cholinesterase inhibitory activities in essential oils of Salvia species and their constituents. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 2004. 18(4): p. 315-324.
89. Referans89 Orhan, I.E., et al., Assessment of anticholinesterase and antioxidant properties of selected sage (Salvia) species with their total phenol and flavonoid contents. Industrial Crops and Products, 2013. 41: p. 21-30.
90. Referans90 Orhan, I. and M. Aslan, Appraisal of scopolamine-induced antiamnesic effect in mice and in vitro antiacetylcholinesterase and antioxidant activities of some traditionally used Lamiaceae plants. Journal of Ethnopharmacology, 2009. 122(2): p. 327-332.
91. Referans91 Şenol, F.S., et al., Evaluation of cholinesterase inhibitory and antioxidant activities of wild and cultivated samples of sage (Salvia fruticosa) by activity-guided fractionation. Journal of medicinal food, 2011. 14(11): p. 1476-1483.
92. Referans92 Topçu, G., et al., Terpenoids, essential oil composition, fatty acid profile, and biological activities of Anatolian Salvia fruticosa Mill. Turkish Journal of Chemistry, 2013. 37(4): p. 619-632.
93. Referans93 Mahdy, K., et al., Effect of some medicinal plant extracts on the oxidative stress status in Alzheimer’s disease induced in rats. Eur Rev Med Pharmacol Sci, 2012. 16(3): p. 31-42.
94. Referans94 Perfumi, M., N. Arnold, and R. Tacconi, Hypoglycemic activity of Salvia fruticosa Mill. from Cyprus. Journal of ethnopharmacology, 1991. 34(2-3): p. 135-140.
95. Referans95 Arabiyat, S., et al., Antilipolytic and hypotriglyceridemic effects of dietary Salvia triloba Lf (Lamiaceae) in experimental rats. Tropical Journal of Pharmaceutical Research, 2016. 15(4): p. 723-728.
96. Referans96 Cam, M.E., et al., Antidiabetic effects of Salvia triloba and Thymus praecox subsp. skorpilii var. skorpilii in a rat model of streptozotocin/nicotinamide-induced diabetes. Marmara Pharmaceutical Journal, 2017. 21(4): p. 818-827.
97. Referans97 Elbetieha, A., et al., Reproductive toxicity potentials of Salvia fruticosa (Labiatae) in rats. Journal of ethnopharmacology, 1998. 61(1): p. 67-74.
98. Referans98 Daradka, H.M., The effect of Salvia fruticosa ingestion on male rat sexual behavior. Res J Biol Sci, 2009. 4(12): p. 1288-91.
99. Referans99 Todorov, S., et al., Experimental pharmacological study of three species from genus Salvia. Acta physiologica et pharmacologica Bulgarica, 1984. 10(2): p. 13-20.
100. Referans100 Anwar, M.A., et al., Salvia fruticosa induces vasorelaxation in rat isolated thoracic aorta: role of the PI3K/Akt/eNOS/NO/cGMP signaling pathway. Scientific reports, 2017. 7(1): p. 1-14.
101. Referans101 Millet, Y., et al., Toxicity of some essential plant oils. Clinical and experimental study. Clinical toxicology, 1981. 18(12): p. 1485-1498.
102. Referans102 Millet, Y., et al., Experimental study of the toxic convulsant properties of commercial preparations of essences of sage and hyssop (author's transl). Revue d'electroencephalographie et de neurophysiologie clinique, 1979. 9(1): p. 12-18.
103. Referans103 Elisabetsky, E., J. Marschner, and D.O. Souza, Effects of linalool on glutamatergic system in the rat cerebral cortex. Neurochemical research, 1995. 20(4): p. 461-465.
104. Referans104 Farhat, G., N. Affara, and H. Gali-Muhtasib, Seasonal changes in the composition of the essential oil extract of East Mediterranean sage (Salvia libanotica) and its toxicity in mice. Toxicon, 2001. 39(10): p. 1601-1605.
105. Referans105 Harmancı, K., et al., Elma yağının yanlış kullanımına bağlı gelişen kimyasal pnömonili bir olgu. Asthma Allergy Immunol, 2011. 9: p. 101-1.
106. Referans106 Gündüz, S., et al., Acı elma yağının yanlış kullanımına bağlı dört zehirlenme vakası. Dicle Tıp Dergisi, 2016. 43(2): p. 364-366.