Selection of Root-Knot Nematod Resistance in Inbred Tomato Lines Using CAPS Molecular Markers

Year 2018, Volume: 1 Issue: 1, 10 - 16, 20.07.2018

Zafer Secgin , Yunus Emre Arvas , Simon Peter Ssendawula Yilmaz Kaya

https://doi.org/10.38001/ijlsb.427056

Cited By: 3

Abstract

Tomato root-knot nematode, is one of the most
important disease agents in tomatoes (Solanum lycopersicum).  A practical and effective method for
controlling this disease is by means of tomato genotypes with resistance genes
against this disease.  Tomato plants are offered resistance to root-knot
nematode by Mi gene. Phenotypically observing genetic lines in terrestrial
conditions is difficult and time consuming due to concerns about the transfer
of this agent to other plants and their stability. By using the molecular
markers related to resistance genes, this problem can be avoided and the
selection efficiency can be increased. In this project, 34 inbred lines were
tested using 1 CAPS marker associated with Mi gene, resistant and susceptible
genotypes were identified.  In the study,
34 genotypes were not found to be homozygous resistant (MI / MI) genotypes
whereas 7 genotypes were found to be heterozygous resistant (MI / mi) and 27
genotypes (mi / mi)homozygous susceptible. As a result of this study, root-knot
nematode is the basis for developing resistant varieties.

Keywords

Tomato, Mi gene

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