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Chemical Composition of Trigonella foenum-graecum Seeds and Inhibitory Activity of Their Seeds Oil Against Some Microbes

Year 2018, Volume: 1 Issue: 2, 75 - 83, 18.12.2018

Abstract



The study aimed to
know the chemical composition of fenugreek seeds and test the inhibitory
effectiveness of the extracts of the seed oil on the types of bacteria and
yeast. The results of the chemical analysis of the circuit showed that the
moisture content of 4.90%, fiber 6.55%, ash 3.35%, protein 27.50%, fat 4.50%,
and carbohydrates 42.26%. The physiochemical properties of fenugreek seed oil
were similar to edible oil. Plant extracts were obtained by extracting oil with
hexane at concentrations 40, 80 and 100%. The inhibitory activity was tested
against the experimental cells that included the Gram negative: Escherichia coli O157: H7s, Samonella. typhimurium ,. Gram positive:
Staphylococcus aureus Aspergillus niger. Antibody activity was
highly effective against living organisms, specially against E.Coli at which the aortic diameter
reached the highest concentration (100%) 21 mm.






References

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  • 2. Khosla, P., Gupta, D.D., & Nagpal, R.K.(1995). Effect of Trigonella foenum graecum (Fenugreek) on serum lipids in normal and diabetic rats. International Journal of Pharmacology; 27, 89-93.
  • 3. Kaviarasan, S., Ramamurty, N., Gunasekaran. P., Varalakshmi, E.,& Anuradha, C.V. Fenugreek.(2006). (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism; Vol. 41, No.3, 267-273
  • 4. Ramachandraiah, O.S., Reddy, P.N., Azeemoddin, G., Ramayya, D.A., &Rao, S.D.T.( 1986).Essential and fatty oil content in umbelliferous and fenugreek seeds of Andhra Pradesh habitat. Indian Cocoa, Arecanut and spices Journal ; 10(1), 12. 5. El Bairi K, Ouzir M, Agnieszka N, Khalki L. (2017)Anticancer potential of Trigonella foenum graecum: cellular and molecular targets. Biomed Pharmacother.;90:479–91.
  • 6. Gupta VK, Sharma SK.(2006) Plants as natural antioxidants. Nat Prod Rad.;5:326–34
  • 7. Mehta B.K., Verma M., Gupta M.J. (2008). Novel lipid constituents identified in seed of Nigella sativa Linn. Braz Chem Soc; 19 (3): 458-462.
  • 8. Cheikh-Rouhou, S., Besbes, S., Lognay, G., Blecker, C., Deroanne, C., & Attia, H. Sterol(2008). composition of black cumin (Nigella sativa L.) and Aleppo pine (Pinus halpensis Mill.) seed oils. J Food Comp Anal ; 21(2): 162-168.
  • 9. Parthasarathy, V.A., Chempakam, B., & Zachariah, T.J. (2008). "Chemistry of Spices". CAB International .
  • 10. Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Damanhouri, Z.A., Anwar.F. A(2013). review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed; 3(5): 337-352.
  • 11. El-Hadiyah, T.M., Raza, M., Mohammed, O.Y., & Abdallah, A.A.(2003). Evaluation of Nigella sativa Seed Constituents for Their in vivo Toxicity in Mice. Natural Product Sciences; 9(1): 22-27
  • 12. Anuradha C.V;( 2001) Ravikumar P. Indian J Physiol Pharmacol., 45, 408–420
  • 13. P. Khosla,P D. D. Gupta, and R. K Nagpal(1995). Int. J.of Pharmacol., 27-89
  • 14. Cw Fetrow; JR. Avila (1999) Professional's Handbook of Complementary and Aternative Medicines. Springhouse, PA: Springhouse Corporation.
  • 15. Kaviarasan, S., Ramamurty, N., Gunasekaran. P., Varalakshmi, E.,& Anuradha, C.V.(2006) Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism ; Vol. 41, No.3, 267-273
  • 16. G.Ribes; Y Sauvaire; CD Costa; JC Baccou; MM Loubatieres-Mariani.(1986).proc soc Exp Biol Med,182, 159–166.
  • 17. AOAC (2005). Official and Tentative Method. 3rd edn. American Oil Chemist’s Society (AOCS), Chpaign, U.S.A.
  • 18. Atlas, R.A.,Parks,L.C.&Brown,A.E.(1995). Laboratory manual experimental microbiology . 1st ed. Mosby-year book :312.
  • 19. El Tahir, KE.H., Al Tahir, A.Y., & Ageel, A.M. (1999).Pharmacological studies on Sesame an Nigella sativa fixed oils: effects on the sensitivities of the adrenoceptors, baroreceptors, platelets and uterus of the rat. Saudi Pharm. J.,; 7, 205-215.
  • 20. Eloff, J. (1998).Which extract should be used for the screening and isolation of antimicrobial compounds from plants. J. Ethnopharm ; 60: 1-8.
  • 21. Crespo, M. E., Jimenez, J., Gomis, E., & Navarro, C. (1990). Antibacterial activity of the essential oil of Thymus serpylloides subspecies gadorensis. Microbios, 61(248-249), 181-184.
  • 22. Han, J. S., Shin, D. H., & Baek, N. I. (2001). Identification of growth inhibitory substance on food-borne microorganisms from commiphora molmol Engl. and its application to food products. Korean Journal of Food Science and Technology, 33(4), 401-408.
  • 23. Mounir, Z. Awadalla, G., Hudson, J. and David, A.T. and South gole (1978). The composition of some native Egyption foodstuffs. Plants foods for man, 2, 147-151
  • 24. Abdel Aal, Y., Shehat M.H. and Elmahdy, A.R. (1985).Chemical and functional properties of some legume powder. Egyptian Journal of Food Science 13(s) ;201-205.
  • 25. Haram, M.A. (1991). The protein quality of Sudanese fenugreek seeds. M. Sc.Thesis, University of Khartoum. Sudan.
  • 26. Shankaracharya, N.B. and Nalarjan, C.P. (1972). Fenugreek chemical composition and values Indian spices (India) (1): 2-12.
  • 27. Abdel-Hamid, M.R., Alalla, R.K. and Moue, Z.N. (1984). “Chemical studies on Egyptian fenugreek seed” Ann. Agric. Sc. Ain Shams University 29(1): 43-60.
  • 28. Sulieman, A. Elhadi. (1995). Fenugreek Supplemented Backed Products, Quality Aspects. M. Sc. Thesis, University of Gezira, Wad Medani, Sudan.
  • 29. Abdel-Ala, M.H. and Rahma, E.H. (1986). “Change in grass chemical composition with emphasis on lipid and protein fractions during germination of fenugreek seed” Food Chemistry 22:193-207.
  • 30. Badreldin, L. E. (2006). Chemical composition of ginger(Zingiber officinale Rose) and detection of antimicrobial activity of its oil. M.Sc.Thesis, University of Gezira.
  • 31. Abdel Moneim E. S, Heba E. A, Awad M. A, (2008). The Chemical Composition of Fenugreek (Trigonella foenum graceum L) and the Antimicrobial Properties of its Seed Oil. Gezira j. of eng. & applied sci.3 (2) :52– 71.
  • 32. Almahna , E.K.H., (2002) .Effect of some Iraqi plant extracts on microorganisms isolated from different body regions. Master Thesis, Faculty of Science, Mustansiriya University.
  • 33. Aksoy, H. M., Kaya, Y., Ozturk, M., Secgin, Z., Onder, H., and Okumus, A. (2017). Pseudomonas putida–induced response in phenolic profile of tomato seedlings (Solanum lycopersicum L.) infected by Clavibacter michiganensis subsp. michiganensis. Biol. Control 105, 6–12. doi: 10.1016/j.biocontrol.2016.11.001.
Year 2018, Volume: 1 Issue: 2, 75 - 83, 18.12.2018

Abstract

References

  • 1. Parthasarathy, V.A., Chempakam, B., & Zachariah, T.J.(2008) "Chemistry of Spices". CAB International .
  • 2. Khosla, P., Gupta, D.D., & Nagpal, R.K.(1995). Effect of Trigonella foenum graecum (Fenugreek) on serum lipids in normal and diabetic rats. International Journal of Pharmacology; 27, 89-93.
  • 3. Kaviarasan, S., Ramamurty, N., Gunasekaran. P., Varalakshmi, E.,& Anuradha, C.V. Fenugreek.(2006). (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism; Vol. 41, No.3, 267-273
  • 4. Ramachandraiah, O.S., Reddy, P.N., Azeemoddin, G., Ramayya, D.A., &Rao, S.D.T.( 1986).Essential and fatty oil content in umbelliferous and fenugreek seeds of Andhra Pradesh habitat. Indian Cocoa, Arecanut and spices Journal ; 10(1), 12. 5. El Bairi K, Ouzir M, Agnieszka N, Khalki L. (2017)Anticancer potential of Trigonella foenum graecum: cellular and molecular targets. Biomed Pharmacother.;90:479–91.
  • 6. Gupta VK, Sharma SK.(2006) Plants as natural antioxidants. Nat Prod Rad.;5:326–34
  • 7. Mehta B.K., Verma M., Gupta M.J. (2008). Novel lipid constituents identified in seed of Nigella sativa Linn. Braz Chem Soc; 19 (3): 458-462.
  • 8. Cheikh-Rouhou, S., Besbes, S., Lognay, G., Blecker, C., Deroanne, C., & Attia, H. Sterol(2008). composition of black cumin (Nigella sativa L.) and Aleppo pine (Pinus halpensis Mill.) seed oils. J Food Comp Anal ; 21(2): 162-168.
  • 9. Parthasarathy, V.A., Chempakam, B., & Zachariah, T.J. (2008). "Chemistry of Spices". CAB International .
  • 10. Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Damanhouri, Z.A., Anwar.F. A(2013). review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed; 3(5): 337-352.
  • 11. El-Hadiyah, T.M., Raza, M., Mohammed, O.Y., & Abdallah, A.A.(2003). Evaluation of Nigella sativa Seed Constituents for Their in vivo Toxicity in Mice. Natural Product Sciences; 9(1): 22-27
  • 12. Anuradha C.V;( 2001) Ravikumar P. Indian J Physiol Pharmacol., 45, 408–420
  • 13. P. Khosla,P D. D. Gupta, and R. K Nagpal(1995). Int. J.of Pharmacol., 27-89
  • 14. Cw Fetrow; JR. Avila (1999) Professional's Handbook of Complementary and Aternative Medicines. Springhouse, PA: Springhouse Corporation.
  • 15. Kaviarasan, S., Ramamurty, N., Gunasekaran. P., Varalakshmi, E.,& Anuradha, C.V.(2006) Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol-induced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism ; Vol. 41, No.3, 267-273
  • 16. G.Ribes; Y Sauvaire; CD Costa; JC Baccou; MM Loubatieres-Mariani.(1986).proc soc Exp Biol Med,182, 159–166.
  • 17. AOAC (2005). Official and Tentative Method. 3rd edn. American Oil Chemist’s Society (AOCS), Chpaign, U.S.A.
  • 18. Atlas, R.A.,Parks,L.C.&Brown,A.E.(1995). Laboratory manual experimental microbiology . 1st ed. Mosby-year book :312.
  • 19. El Tahir, KE.H., Al Tahir, A.Y., & Ageel, A.M. (1999).Pharmacological studies on Sesame an Nigella sativa fixed oils: effects on the sensitivities of the adrenoceptors, baroreceptors, platelets and uterus of the rat. Saudi Pharm. J.,; 7, 205-215.
  • 20. Eloff, J. (1998).Which extract should be used for the screening and isolation of antimicrobial compounds from plants. J. Ethnopharm ; 60: 1-8.
  • 21. Crespo, M. E., Jimenez, J., Gomis, E., & Navarro, C. (1990). Antibacterial activity of the essential oil of Thymus serpylloides subspecies gadorensis. Microbios, 61(248-249), 181-184.
  • 22. Han, J. S., Shin, D. H., & Baek, N. I. (2001). Identification of growth inhibitory substance on food-borne microorganisms from commiphora molmol Engl. and its application to food products. Korean Journal of Food Science and Technology, 33(4), 401-408.
  • 23. Mounir, Z. Awadalla, G., Hudson, J. and David, A.T. and South gole (1978). The composition of some native Egyption foodstuffs. Plants foods for man, 2, 147-151
  • 24. Abdel Aal, Y., Shehat M.H. and Elmahdy, A.R. (1985).Chemical and functional properties of some legume powder. Egyptian Journal of Food Science 13(s) ;201-205.
  • 25. Haram, M.A. (1991). The protein quality of Sudanese fenugreek seeds. M. Sc.Thesis, University of Khartoum. Sudan.
  • 26. Shankaracharya, N.B. and Nalarjan, C.P. (1972). Fenugreek chemical composition and values Indian spices (India) (1): 2-12.
  • 27. Abdel-Hamid, M.R., Alalla, R.K. and Moue, Z.N. (1984). “Chemical studies on Egyptian fenugreek seed” Ann. Agric. Sc. Ain Shams University 29(1): 43-60.
  • 28. Sulieman, A. Elhadi. (1995). Fenugreek Supplemented Backed Products, Quality Aspects. M. Sc. Thesis, University of Gezira, Wad Medani, Sudan.
  • 29. Abdel-Ala, M.H. and Rahma, E.H. (1986). “Change in grass chemical composition with emphasis on lipid and protein fractions during germination of fenugreek seed” Food Chemistry 22:193-207.
  • 30. Badreldin, L. E. (2006). Chemical composition of ginger(Zingiber officinale Rose) and detection of antimicrobial activity of its oil. M.Sc.Thesis, University of Gezira.
  • 31. Abdel Moneim E. S, Heba E. A, Awad M. A, (2008). The Chemical Composition of Fenugreek (Trigonella foenum graceum L) and the Antimicrobial Properties of its Seed Oil. Gezira j. of eng. & applied sci.3 (2) :52– 71.
  • 32. Almahna , E.K.H., (2002) .Effect of some Iraqi plant extracts on microorganisms isolated from different body regions. Master Thesis, Faculty of Science, Mustansiriya University.
  • 33. Aksoy, H. M., Kaya, Y., Ozturk, M., Secgin, Z., Onder, H., and Okumus, A. (2017). Pseudomonas putida–induced response in phenolic profile of tomato seedlings (Solanum lycopersicum L.) infected by Clavibacter michiganensis subsp. michiganensis. Biol. Control 105, 6–12. doi: 10.1016/j.biocontrol.2016.11.001.
There are 32 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Mohammed Abed 0000-0002-4609-4757

Sara T. Hadi This is me

Noor j. Fadhil This is me

Publication Date December 18, 2018
Published in Issue Year 2018 Volume: 1 Issue: 2

Cite

EndNote Abed M, Hadi ST, Fadhil Nj (December 1, 2018) Chemical Composition of Trigonella foenum-graecum Seeds and Inhibitory Activity of Their Seeds Oil Against Some Microbes. International Journal of Life Sciences and Biotechnology 1 2 75–83.



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