Study of chemical composition and antimicrobial properties of Rosemary (Rosmarinus officinalis) essential oil on Staphylococcus aureus and Escherichia coli in vitro

Year 2020, Volume: 3 Issue: 1, 62 - 69, 15.04.2020

Abolfazl Jafarı-sales , Mehrdad Pashazadeh

https://doi.org/10.38001/ijlsb.693371

Cited By: 29

Abstract

Nowadays, with increasing bacterial resistance, finding new antimicrobial agents to replace herbal remedies with less side effects than conventional drugs has provided. The aim of this study was to investigate the chemical composition and antimicrobial properties of Rosemary (Rosmarinus officinalis) essential oil on Staphylococcus aureus and Escherichia coli in vitro. In this experimental study, rosemary was prepared from Tabriz city and the essential oil was extracted after drying by water distillation using Clevenger apparatus. The essential oil components were identified by Gas chromatography mass spectrometry (GC-MS). Essential oil of 10, 5, 2.5, 1.25, 0.625 and 0.312% of essential oil was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Based on the results of GC-MS analysis, 19 compounds were identified, of which 1.8 Cineole and α-pinen had the highest essential oil composition. MIC and MBC of essential oils were obtained on S. aureus (0.625 and 1.25%) and E. coli (1.25 and 2.5%), respectively. The results of this study showed that rosemary essential oil has remarkable antibacterial activity and can be used as a suitable replacement for synthetic antibiotics.

Keywords

: Antibacterial effects, Essential oils, Rosemary, Pathogenic bacteria

References

• 1. Rajaee Maleki S. The In Vitro Inhibitory Effects of the Rosemary Essential Oil on Some Gram Positive and Negative Bacteria. scientific journal of ilam university of medical sciences. 2016 Jun 10;24(2):80-9. 2. Alviano DS, Alviano CS. Plant extracts: search for new alternatives to treat microbial diseases. Current pharmaceutical biotechnology. 2009 Jan 1;10(1):106-21. 3. Jafari-Sales A, Hossein-Nezhad P. Antimicrobial effects of Rosmarinus officinalis methanolic extract on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in laboratory conditions. Journal of Medicinal and Chemical Sciences. 2019 Sep 2:103-8. 4. Skaltsa HD, Lazari DM, Chinou IB, Loukis AE. Composition and antibacterial activity of the essential oils of Stachys candida and S. chrysantha from southern Greece. Planta medica. 1999 Apr;65(03):255-6. 5. Malini M, Abirami G, Hemalatha V, Annadurai G. Antimicrobial activity of ethanolic and aqueous extracts of medicinal plants against waste water pathogens. International Journal of Research in Pure and Applied Microbiology. 2013;3(2):40-2. 6. Skaltsa HD, Demetzos C, Lazari D, Sokovic M. Essential oil analysis and antimicrobial activity of eight Stachys species from Greece. Phytochemistry. 2003 Oct 1;64(3):743-52. 7. Digrak M, Alma MH, Ilçim A. Antibacterial and antifungal activities of Turkish medicinal plants. Pharmaceutical Biology. 2001 Jan 1;39(5):346-50. 8. Özcan M. Antioxidant activities of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil. Journal of medicinal food. 2003 Oct 1;6(3):267-70. 9. Bozin B, Mimica-Dukic N, Samojlik I, Jovin E. Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. Journal of agricultural and food chemistry. 2007 Sep 19;55(19):7879-85. 10. Peng Y, Yuan J, Liu F, Ye J. Determination of active components in rosemary by capillary electrophoresis with electrochemical detection. Journal of pharmaceutical and biomedical analysis. 2005 Sep 15;39(3-4):431-7. 11. Simon JE, Chadwick AF. Herbs an indexed bibliography 1971-1980: the scientific literature on selected herbs and aromatic and medicinal plants of the temperature zone. 1984. 12. Martinez-Lirola MJ, González-Tejero MR, Molero-Mesa J. Ethnobotanical resources in the province of Almería, Spain: Campos de Níjar. Economic Botany. 1996 Jan 1;50(1):40-56. 13. Chandler F. Memory stimulat herbal medicine. Can Pharm J1995;28:40-53 14. Sotelo-Felix JI, Martinez-Fong D, Muriel P, Santillan RL, Castillo D, Yahuaca P. Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride-induced acute hepatotoxicity in the rat. Journal of ethnopharmacology. 2002 Jul 1;81(2):145-54. 15. Nieto G, Estrada M, Jordán MJ, Garrido MD, Bañón S. Effects in ewe diet of rosemary by-product on lipid oxidation and the eating quality of cooked lamb under retail display conditions. Food Chemistry. 2011 Feb 15;124(4):1423-9. 16. Nieto G, Bañon S, Garrido MD. Administration of distillate thyme leaves into the diet of Segureña ewes: effect on lamb meat quality. animal. 2012 Dec;6(12):2048-56. 17. Nieto G, Díaz P, Bañón S, Garrido MD. Dietary administration of ewe diets with a distillate from rosemary leaves (Rosmarinus officinalis L.): Influence on lamb meat quality. Meat Science. 2010 Jan 1;84(1):23-9. 18. Okoh OO, Chemical Transformations and Phytochemical Studies of Bioactive Components from Extracts of Rosmarinus Officinalis L 2010: University of Fort Hare. 19. Senatore F. Influence of harvesting time on yield and composition of the essential oil of a thyme (Thymus pulegioides L.) growing wild in Campania (Southern Italy). Journal of agricultural and food chemistry. 1996 May 16;44(5):1327-32. 20. Malakootian M, Hatami B. Survey of Chemical Composition and Antibacterial Activity of Rosmarinus Officinalis Essential oils on Escherichia Coli and Its Kinetic. TB. 2013; 12 (1) :1-13 21. Zaouali Y, Bouzaine T and Boussaid M, Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food and Chemical Toxicology 2010;48:3144-52. 22. Okoh OO, Sadimenko AP, Afolayan AJ. Comparative evaluation of the antibacterial activities of the essential oils of Rosmarinus officinalis L. obtained by hydrodistillation and solvent free microwave extraction methods. Food chemistry. 2010 May 1;120(1):308-12. 23. Linares B, Arráez-Román D, Herrero M, et al., Comparison of different extraction procedures for the comprehensive characterization of bioactive phenolic compounds in Rosmarinus officinalis by reversed-phase high-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight mass spectrometry. Journal of Chromatography2011;1218:7682- 90. 24. Santoyo S, Cavero S, Jaime L, Ibanez E, Senorans FJ, Reglero G. Chemical composition and antimicrobial activity of Rosmarinus officinalis L. essential oil obtained via supercritical fluid extraction. Journal of food protection. 2005 Apr;68(4):790-5. 25. Angioni A, Barra A, Cereti E, Barile D, Coïsson JD, Arlorio M, Dessi S, Coroneo V, Cabras P. Chemical composition, plant genetic differences, antimicrobial and antifungal activity investigation of the essential oil of Rosmarinus officinalis L. Journal of agricultural and food chemistry. 2004 Jun 2;52(11):3530-5. 26. Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian journal of microbiology. 2000 Oct;31(4):247-56. 27. Fu Y, Zu Y, Chen L, Shi X, Wang Z, Sun S, Efferth T. Antimicrobial activity of clove and rosemary essential oils alone and in combination. Phytotherapy research. 2007 Oct;21(10):989-94. 28. Seydim AC, Sarikus G. Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food research international. 2006 Jun 1;39(5):639-44. 29. Tsai PJ, Tsai TH, Ho SC. In vitro inhibitory effects of rosemary extracts on growth and glucosyltransferase activity of Streptococcus sobrinus. Food chemistry. 2007 Jan 1;105(1):311-6. 30. ahmady-asbchin, S., Mostafapour, M. Anti-bacterial interactions Rosemary (Officinalis rosmarinus) and essential oils of lavender (Lavandula stoechas) on two Gram-positive and three Gram- negative bacteria in vitro. Iranian Journal of Cellular and Molecular Researches, 2018; 31(2): 177-187. 31. Soltan Dallal M M, Ghorbanzade Mashkani M, Yazdi M H, Agha Amiri S, Mobasseri G, Abedi Mohtasab T P et al . Antibacterial effects of Rosmarinus officinalis on Methicillin -resistant Staphylococcus aureus isolated from patients and foods. SJKU. 2011; 16 (1) :73-80 32. Mashreghi M, Momtazi F. Comparison of the Antibacterial Effects of Various Concentrations of Alcoholic Extracts of Rosmarinus officinalis, Hypericum perforatum and Carthamus tinctorius on the Growth Phases of Esherichia coli O157. JRUMS. 2012; 11 (2) :103-114