in vitro Study of Antidiabetic Effect of Abrus precatorius Methanol Leaves Extract against Glucose Absorption

Year 2020, Volume: 3 Issue: 2, 117 - 126, 15.08.2020

Hafedh Al-moalemi , Wahid Altowayti Siti Pauliena Mohd Bohari

https://doi.org/10.38001/ijlsb.701093

Cited By: 1

Abstract

Diabetes mellitus is a common chronic systemic disorder characterised by hyperglycaemia as a standard feature. A traditional plant known as Abrus precatorius (AP) has been used for the treatment of type II diabetes mellitus in Malaysia. The potential of the 80% methanolic extract of A. precatorius leaves has been tested in vitro for its α-glucosidase inhibition and its glucose diffusion activity. It was observed that the extraction of A. precatorius leaves exhibit a high α-glucosidase inhibition at the concentrations of 25 and 50 mg/mL (65.4% and 84.6%), respectively, but low inhibition at the concentration of 6.25 to 12.5 mg/mL (25% and 28.2%) when compared to control. Meanwhile, the methanolic extract of A. precatorius slightly affected the glucose diffusion at the concentration of 50 mg/mL (9.5%) within 24 h compared to the control group. These results indicated that the methanolic extract of A. precatorius leaves is capable of inhibiting α-glucosidase activity, besides halting glucose diffusion activity by delaying the glucose absorption in the gut.

Keywords

Abrus precatorius, Diabetes mellitus, Acarbose, Alpha-glucosidase, Glucose diffusion

Supporting Institution

Universiti Teknologi Malaysia

Project Number

Q.J130000.3554.07G36

Thanks

The authors thank Universiti Teknologi Malaysia for the financial support under the Research University Grant (Q.J130000.3554.07G36).

References

• 1. West, I., Radicals and oxidative stress in diabetes. Diabetic Medicine, 2000. 17(3): p. 171-180.
• 2. Sridhar, S., S. Kumari, and A. T Paul, Diabetic complications: A natural product perspective. Pharmaceutical Crops, 2014. 5(1).
• 3. Ogurtsova, K., et al., IDF Diabetes Atlas: Global estimates for the prevalence of diabetes for 2015 and 2040. Diabetes research and clinical practice, 2017. 128: p. 40-50.
• 4. Association, A.D., 2. Classification and diagnosis of diabetes: standards of medical care in diabetes—2018. Diabetes care, 2018. 41(Supplement 1): p. S13-S27.
• 5. Soumya, D. and B. Srilatha, Late stage complications of diabetes and insulin resistance. J Diabetes Metab, 2011. 2(9): p. 1000167.
• 6. Penumala, M., et al., Phytochemical profiling and in vitro screening for anticholinesterase, antioxidant, antiglucosidase and neuroprotective effect of three traditional medicinal plants for Alzheimer’s Disease and Diabetes Mellitus dual therapy. BMC complementary and alternative medicine, 2018. 18(1): p. 77.
• 7. Jones, K., et al., Mapping the intestinal alpha-glucogenic enzyme specificities of starch digesting maltase-glucoamylase and sucrase-isomaltase. Bioorganic & medicinal chemistry, 2011. 19(13): p. 3929-3934.
• 8. Lieberman, M. and A.D. Marks, Marks' basic medical biochemistry: a clinical approach. 2009: Lippincott Williams & Wilkins.
• 9. Sinha, D., et al., Recent status on carbohydrate metabolizing enzyme inhibitors in regulation of diabetes: a mechanism based review. Journal of applied pharmaceutical research, 2015. 3(2): p. 1-7.
• 10. Heacock, P.M., et al., Effects of a medical food containing an herbal α-glucosidase inhibitor on postprandial glycemia and insulinemia in healthy adults. Journal of the American Dietetic Association, 2005. 105(1): p. 65-71.
• 11. Wu, H., et al., Comparative assessment of the efficacy and safety of acarbose and metformin combined with premixed insulin in patients with type 2 diabetes mellitus. Medicine, 2017. 96(35).
• 12. Thongnum, K. and S. Chanthai, Inhibitory reactivity of capsaicin with α-amylase and α-glucosidase related to antidiabetes using molecular docking and quantum calculation methods. Oriental Journal of Chemistry, 2018. 34(5): p. 2211.
• 13. Figueiredo-González, M., et al., α-Glucosidase and α-amylase inhibitors from Myrcia spp.: a stronger alternative to acarbose? Journal of pharmaceutical and biomedical analysis, 2016. 118: p. 322-327.
• 14. Kee, K.T., et al., Screening culinary herbs for antioxidant and α‐glucosidase inhibitory activities. International Journal of Food Science & Technology, 2013. 48(9): p. 1884-1891.
• 15. Mohamed, E.A.H., et al., Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism. BMC complementary and alternative medicine, 2012. 12(1): p. 176.
• 16. Shadhan, R.M. and S.P.M. Bohari, Effects of Hibiscus sabdariffa Linn. fruit extracts on α-glucosidase enzyme, glucose diffusion and wound healing activities. Asian Pacific Journal of Tropical Biomedicine, 2017. 7(5): p. 466-472.
• 17. Bohari, M., et al., Glucose uptake: stimulatory activity of Gynura procumbens in 3T3-F442A adipocytes. Malaysian Medicinal Plant: Chemistry and Biological Activity, 2006.
• 18. Dong, H.-Q., et al., Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α-amylase linked to type 2 diabetes. Food Chemistry, 2012. 130(2): p. 261-266.
• 19. Picot, C., A.H. Subratty, and M.F. Mahomoodally, Inhibitory potential of five traditionally used native antidiabetic medicinal plants on α-amylase, α-glucosidase, glucose entrapment, and amylolysis kinetics in vitro. Advances in Pharmacological Sciences, 2014. 2014.
• 20. Saini, P. and M. Gangwar, Enzyme and free radical inhibitory potentials of ethyl acetate extract of endophytic actinomycete from Syzygium cumini. 2017.
• 21. Edwards, C., et al., Viscosity of food gums determined in vitro related to their hypoglycemic actions. The American journal of clinical nutrition, 1987. 46(1): p. 72-77.
• 22. Basha, S.K. and V.S. Kumari, In vitro antidiabetic activity of psidium guajava leaves extracts. Asian Pacific Journal of Tropical Disease, 2012. 2: p. S98-S100.
• 23. Ortiz-Andrade, R., et al., α-Glucosidase inhibitory activity of the methanolic extract from< i> Tournefortia hartwegiana</i>: An anti-hyperglycemic agent. Journal of ethnopharmacology, 2007. 109(1): p. 48-53.
• 24. Hanefeld, M. and F. Schaper, The role of alpha-glucosidase inhibitors (acarbose), in Pharmacotherapy of Diabetes: New Developments. 2007, Springer. p. 143-152.
• 25. Shim, Y.-J., et al., Inhibitory effect of aqueous extract from the gall of< i> Rhus chinensis</i> on alpha-glucosidase activity and postprandial blood glucose. Journal of ethnopharmacology, 2003. 85(2): p. 283-287.
• 26. Rege, A. and A.S. Chowdhary, Evaluation of Alpha-Amylase and Alpha-Glucosidase Inhibitory Activities of Ocimum sanctum Linn. International Journal of Pharmaceutical Sciences Review & Research, 2014. 25(1).
• 27. Joshi, S.R., et al., Therapeutic potential of α-glucosidase inhibitors in type 2 diabetes mellitus: an evidence-based review. Expert opinion on pharmacotherapy, 2015. 16(13): p. 1959-1981.
• 28. Bastaki, A., Diabetes mellitus and its treatment. International journal of Diabetes and Metabolism, 2005. 13(3): p. 111.
• 29. Chaudhuri, A. and S. Sharma, Evaluation of antidiabetic activity of polyherbal formulation in streptozotocin-induced diabetic rats. UK Journal of Pharmaceutical and Biosciences, 2016. 4(5): p. 01-06.
• 30. Chiasson, J.-L., et al., Acarbose for prevention of type 2 diabetes mellitus: the STOP-NIDDM randomised trial. The Lancet, 2002. 359(9323): p. 2072-2077.
• 31. Sekar, V., et al., Mangiferin from Mangifera indica fruits reduces post-prandial glucose level by inhibiting α-glucosidase and α-amylase activity. South African journal of botany, 2019. 120: p. 129-134.
• 32. Alagesan, K., et al., Identification of α-glucosidase inhibitors from Psidium guajava leaves and Syzygium cumini Linn. seeds. International Journal of Pharma Sciences and Research, 2012. 3(2): p. 316-322.
• 33. Gallagher, A., et al., The effects of traditional antidiabetic plants on in vitro glucose diffusion. Nutrition research, 2003. 23(3): p. 413-424.
• 34. Edwards, C., I. Johnson, and N. Read, Do viscous polysaccharides slow absorption by inhibiting diffusion or convection? European Journal of Clinical Nutrition, 1988. 42(4): p. 307-312.
• 35. Qujeq, D. and A. Babazadeh, The entrapment ability of aqueous and ethanolic extract of Teucrium polium: glucose diffusion into the external solution. International journal of molecular and cellular medicine, 2013. 2(2): p. 93.
• 36. Preedy, V.R., Dietary Sugars: Chemistry, Analysis, Function and Effects. 2012: Royal Society of Chemistry.
• 37. Ogbonnia, S. and C. Anyakora. Chemistry and Biological Evaluation of Nigerian Plants with Anti-Diabetic Properties. in ACS symposium series. 2009. Oxford University Press.