An investigation of the probiotic properties of Lactobacillus fermentum

Year 2020, Volume: 3 Issue: 2, 180 - 191, 15.08.2020

Serap Çetinkaya Samet Kocabay Ali Yenidunya

https://doi.org/10.38001/ijlsb.725520

Cited By: 1

Abstract

The study assessed some of the probiotic characteristics of an isolate (AB4 26) of Lactobacillus fermentum. AB4 26 was isolated from a faecal sample of an infant, fed with breast milk. The results indicated that the isolate had acceptable survival rates in gastric juice both in the presence and absence of pepsin. It also displayed acceptable sensitivity rates to eleven different antibiotics. Hydrophobicity test showed that the isolate had a good capacity to adhere to xylene. It could also destroy sodium salts. AB4 26 displayed the least survival rates in bile salt. These initial findings could suggest that infant faeces and breast milk could serve as good sources of probiotic organisms.

Keywords

Acid tolerance, Lactobacillus fermentum, Probiotic, Surface hydrophobicity

Project Number

F-498

References

• 1. Ahmad, I., Ahmad, F., Pichtel, J., Microbes and Microbial Technology, Springer. 2011.
• 2. Binns, N., Probiotics, Prebiotics and The Gut Microbiota, ILSI Europe Concise Monograph Series, International Life Sciences Institute. 2013.
• 3. Hoffman, F., A., Heimbach, J. T., Sanders, M. E. and Hibberd, P. L. Executive Summary Scientific and Regulatory Challenges of Development of Probiotics as Foods and Drugs, Science and Regulation of Probiotics,.2008:46.
• 4. Tahri, K., Grill, J. P., Schneider, F., Bifidobacteria strain behaviour toward cholesterol: coprecipitation with bile salts and assimilation. Curr. Microbiol. 1996. 33, 187-193.
• 5. Floch, M. H., Binder, H. J., Filburn, B. and Gershengoren, W. The effect of bile acids on intestinal microflora. Am J Clin Nutr. 1972;25:1418-26.
• 6. Mishra, C. and Lambert, J. Production of antimicrobial substances by probiotics. Asian Pacific J Clinic Nutr. 1996. 5(1): 20-24.
• 7. Rolfe, R. D., The role of probiotic cultures in the control of gastrointestinal health. J Nutr 2000;130:396S–402S.
• 7. Reid, G. and Bruce, A.W. Selection of Lactobacillus strains for urogenital probiotic applications. J. Infect. Dis. 2001. 183.
• 8. Barbosa, T.M. and Levy, S. B., The impact of antibiotic use on resistance development and persistence. Drug Resist. Updat. 2000. 3, 303–311.
• 9. Klaenhammer, T. R., Genetics of intestinal lactobacilli. Int. Dairy J. 1995. 5, 1019–1058.
• 10. Delves-Broughton, J., Blackburn, P., Evans, R.J., Hugenholtz, J., Applications of the bacteriocin, nisin. Antonie van Leeuwenhoek. 1996. 69, 193–202.
• 11. Reid, G., Bruce, A. W., McGroarty, J. A., Cheng, K. J., and Costerton, J. W., Is there a role for lactobacilli in prevention or urogenital and intestinal infection? Clin. Microbiol. Rev., 1990; 3(4): 335–344.
• 12. Kolenbrander, P. E. Intergeneric coaggregation among human oral bacteria and ecology of dental plaque. Annu. Rev. Microbiol. 1988. 42:627–56.
• 13. TURHAN YILMAZ, F., Determination of Potential Probiotic Properties of Some Strains of Lactic Acid Bacteria Isolated in Vaginal Secretion, Ankara University, Biotechnology Institute, Ph.D. Thesis. 2011.
• 14. Bulut, C., Gunes, H., Okuklu, B., Harsa, S., Kilic, S., Coban, H.S., Yenidunya, A.F., Homofermentative lactic acid bacteria of a traditional cheese, Comlek peyniri from Cappadocia region. J. Dairy Res. 2005. 72, 19–24.
• 15. Yenidünya, A. F., Kitapcı, A. Y. And Cetin, A., A comparative study on colonic and milk bacteria by double-digested 16S rRNA gene amplicons, Biotechnol. Biotechnol. Equip. 2010. 1310–2818.
• 16. Iver, R., Tomar, S. K., Kapila, S., Mani, J., Sign, R., Probiotic properties of folate producing Streptococcus thermophilus strains. Food Research International. 2010. 43:103-110.
• 17. Elli, M., Cellegari, M., L., Ferrari, S., Bessi, E., Cattivelli, D., Soldi, S., Survival of yoghurt bacteria in the human gut, Applied and Environmental Microbiology 2006. 72: 5113-5117.
• 18. Zoral, S., İNSAN KAYNAKLI Lactobacillus Spp SUŞLARININ PROBİYOTİK ÖZELLİKLERİNİN BELİRLENMESİ, Ahi Evran Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Yüksek Lisans Tezi. 2013.
• 19. Ghosh, K., Ray, M., Adak, A., Halder, S., K., Das, A., Jana, A., Parua, S., Role of probiotic Lactobacillus fermentum KKL1 in the preparation of a rice based fermented beverage, Bioresource Technology. 2015. 188: 161–168.
• 20. Corcoran, B. M., Stanton, C., Fitzgerald, G. F., Ross, R. P., Survival of Probiotic Lactobacilli in Acidic Environments Is Enhanced in the Presence of Metabolizable Sugars, Applied And Enivronmental Microbiology. 2005. 3060–3067.
• 21. Ahmad, V., Iqbal, A. N. M. Z., Haseeb, M., Khan, M. S., Antimicrobial potential of bacteriocin producing Lysinibacillus jx416856 against foodborne bacterial and fungal pathogens, isolated from fruits and vegetable waste, Anaerobe. 2014. 27: 87-95.