Effects of Salt Stress on Morpho-Physiological and Biochemical Characters of Lentisk (P. lentiscus L.)

Abstract

Among abiotic stresses salinity is the most detrimental factor in limiting crop productivity. In this study, the effect of different sodium chloride (NaCl) concentrations (0, 50, 100, 150, 200, 250 mM) on growth and physiological parameters of Pistacia lentiscus L. seedlings raised in in vitro condition for 4 weeks was investigated. For this purpose the seeds of Lentisk were germinated in Murashige and Skoog, (1962) basal mediums containing different NaCl concentrations. The morphological, physiological and biochemical changes that occurred in the seedlings were measured and recorded after exposure to salt stress. These results show that visible leaf damage of Lentisk seedlings are affected by high salt concentrations. High salinity concentrations significantly reduce root and stem lengths, relative water content (RWC), total chlorophyll, Cl-a, Cl-b and carotenoid values after the culture periods. At 250 mM salt concentration, root and stem growth were found to be completely stopped. The parameters over the 50 mM salt concentrations caused in decrease in the activity of the antioxidant enzyme peroxidase (POD).

Keywords

• in vitro,salt stress,morphology,P. lentiscus L.,physiology

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