Effects of Salt Stress on Morpho-Physiological and Biochemical Characters of Lentisk (P. lentiscus L.)

Year 2019, Volume: 3 Issue: 1, 20 - 31, 01.06.2019

Emine Ayaz Tilkat Alevcan Kaplan , Engin Tilkat , Gurbet Bağlamış Ahmet Onay

Abstract

Among abiotic
stresses salinity is the most detrimental factor in limiting crop productivity. In this study, the effect of
different sodium chloride (NaCl) concentrations (0, 50, 100, 150, 200, 250 mM)
on growth and physiological parameters of Pistacia
lentiscus L. seedlings raised in in vitro condition for 4 weeks was
investigated. For this purpose the seeds of Lentisk were germinated in
Murashige and Skoog, (1962) basal mediums containing different NaCl
concentrations. The morphological, physiological and biochemical changes that
occurred in the seedlings were measured and recorded after exposure to salt
stress. These results show that visible
leaf damage of Lentisk seedlings are affected by high salt
concentrations. High salinity concentrations significantly reduce root and stem
lengths, relative water content (RWC), total chlorophyll, Cl-a, Cl-b and
carotenoid values after the culture periods. At 250 mM salt concentration, root
and stem growth were found to be completely stopped. The parameters over the
50 mM salt concentrations caused in decrease in the activity of the antioxidant
enzyme peroxidase (POD).

Keywords

in vitro, salt stress, morphology, P. lentiscus L., physiology

References

• 1. Abbaspour H, Afshari H, Abdel-Wahhab A. 2012. Influence of salt stress on growth, pigments, soluble sugars and ion accumulation in three pistachio cultivars. Journal of Medicinal Plant. Research 6, 2468- 2473.
• 2. Adish M, Fekri M, Hokmabadi H. 2010. Response of Badami-Zarand pistachio rootstock to salinity stress. Int J Nuts Related Sci 1:1-111.
• 3. Arnon, DI. 1949. Copper Enzymes in Isolated Chloroplasts Polyphanoloxidase in Beta vulgaris. Plant Physiol 24:1–15.
• 4. Asadollahi Z, Mozaffari V. 2013. Effects of salinity and manganese on growth and chemical composition of pistachio (Pistacia vera L.) seedlings in perlite medium. EJGCST; 3:13-28.
• 5. Ashraf MY, Bhatti AS. 2000. Effect of salinity on growth and chlorophyll content in rice. Pak J Ind Res 43:130-131.
• 6. Batool N, Shahzad A, Ilyas N, Noor T. 2014. Plants and salt stress. Int J Agri Crop Sci. 7:582-589.
• 7. Ben Hamed S, Lefi E. 2015. Dynamics of grpwth and phytomas allocation in seedlings of Pistacia atlantica Desf. versus Pistacia vera L. under salt stress. Int J Agr Agri Res 6:16-27.
• 8. Ben Hassaini H, Fetati A, Hocine AK, Belkhodja M. 2012. Effect of salt stress on growth and accumulation of proline and soluble sugars on plantlets of Pistacia atlantica Desf. subsp. atlantica used as rootstocks. Biotechnol Agron Soc Environ 16:159-165.
• 9. Benmahioul B, Daguin F, Kaid-Harche M. 2009. Effect of salt stress on germination and growth in vitro of the pistachio (Pistacia vera L.). C R Biol 332:752-758.
• 10. Bouriche H, Saidi A, Ferradji A, Belambri SA, Senator A. 2016. Anti-inflammatory and immunomodulatory properties of Pistacia lentiscus extracts. JAPS 6: 140-146.
• 11. Bozorgi M, Memariani Z, Mobli M, et al. 2013. Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk and P. lentiscus): A review of their traditional uses, phytochemistry and pharmacology. Sci World J. 219815.
• 12. Chelli-Chaabouni A, Ben Mosbah A, Maalej M, Gargouri K, Gargouri-Bouzid R, Drira N. 2010.In vitro salinity tolerance of two pistachio rootstocks: Pistacia vera L. and Pistacia atlantica Desf. Environ Exp Bot 69:302-312.
• 13. Cristiano G, Camposeo S, Fracchiolla M, Vivaldi GA, De Lucia B, Cazzato E. 2016. Salinity differentially affects growth and ecophysiology of two mastic tree (Pistacia lentiscus L.) accessions. Forests 7:156.
• 14. Çelik Ö, Atak Ç. 2012. The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties. Turk J Biol, 36:339-356.
• 15. Di paolo, G. 2015. The efficacy of chios mastic (Pistacia lentiscus) to gastritis. Website:http://www.global-antiaging-medicine.com/2015/12/07/the-efficacy-of-chios-mastic-pistacia-lentiscus-to-gastritis [accessed 10 July 2017].
• 16. Diego AM, Marco AO, Carlos AM, José C. 2003. Photosynthesis and activity of superoxide dismutase peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76.
• 17. Feng L. 2011. Comparative study of slat resistance of Pistacia chinensis and Hovenia dulcis Journal of Northwest Forestry University, 3:41-44.
• 18. Ferguson L, Poss PA, Grattan SR, Grieve CM, Want D, Wilson C, Donovan TJ, Chao CT. 2002. Pistachio rootstocks influence scion growth and ion relations under salinity and boron stress.J Amer Soc Hort Sci 127:194-199.
• 19. Hellicar M, Gilligan P. 1994. A guide to the herbs of Cyprus, planted in the Pano AkourdaliaChryseleousaHerbGarden,websitehttp://kypros.org/projects/laona/lentisk.html [accessed 10 July 2017].
• 20. Hokmabadi H, Arzani K, Grierson PF. 2005. Growth, chemical composition and carbon isotope discrimination of pistachio (Pistacia vera L.) rootstocks seedlings in response to salinity. Aust J Agric Res 56:135-144.
• 21. Kahrizi S, Sedighi M, Sofalian O. 2012. Effect of salt stress on proline and activity of antioxidant enzymes in ten Durum wheat cultivars. Ann Biol Res 3:3870-3874.
• 22. Kamiab F, Talaie A, Javanshah A, Khezri M, Khalighi A. 2012. Effect of long-term salinity on growth, chemical composition and mineral elements of pistachio (Pistacia vera cv. Badamizarand) rootstock seedlings. Ann Biol Res 3:5545-5551.
• 23. Kılınç FM, Süzerer V, Özden Çiftçi Y, Onay A, Yıldırım H, Uncuoğlu Altınkut A, Tilkat E, Koc I, Akdemir ÖF, Metin Karakaş Ö. 2015. Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers. Plant Growth Regul 75:75-88.
• 24. Kumar KB, Khan PA. 1982. Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. Ind J Exp Bot 20:412-416.
• 25. Kumar V, Shriram V, Nikam TD, Jawali N, Shitole MG. 2009. Antioxidant enzyme activities and protein profiling under salt stress in indica rice genotypes differing in salt tolerance. Arch Agron Soil Sci 55:379-394.
• 26. Lichtenthaler HK, Wellburn AR. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc T 11:591- 592.
• 27. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1977. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265.
• 28. M'barek BN, Cheick-M'hamed H, Raoudha A, Bettaib-Kaab L. 2007. Relationship between peroxidase activity and salt tolerance during barley seed germination. Agron J 6:433-438.
• 29. Miljuš-Djukić J, Stanisavljević N, Radović S, Jovanović 1Ž, Mikić A, Maksimović V. 2013. Differential response of three contrasting pea (Pisum arvense, P. sativum and P. fulvum) species to salt stress: assessment of variation in antioxidative defence and miRNA expression. Aust J Crop Scie 7: 2145-2153.
• 30. Mohanty B, Ong BL. 2003. Contrasting effects of submergence in light and dark on pyruvate decarboxylase activity in roots of rice lines differing in submergence tolerance. Ann Bot 91:291-300.
• 31. Musyimi DM. 2015. Ecology and physiology of plant growth in relation to soil salinity. Sci Agri 11:26-31.
• 32. Pirbalouti AG, Aghaee K. 2011. Chemical composition of essential oil of Pistacia khinjuk stocks grown in Bakhtiari Zagross Mountains, Iran. Elect J Biol 7:67–69.
• 33. Ranjbar A, van Damme P, Samson R, Lemeur R. 2002. Leaf water status and photosynthetic gas exchange of Pistacia khinjuk and P. mutica exposed to osmotic drought stress. Acta Hort, 591:423-428.
• 34. Rezaeyan S, Pourmajidian MR, Jalilvand H, Parsakhoo A. 2009. Growth parameters of Pistacia atlantica Desf under different soil conditions in Iran. Afr J Plant Sci 3:184-189.
• 35. Sairam RK, Rao KV, Srivastava GC. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci 163:1037-1046.
• 36. Shrivastava P, Kumar R. 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J Biol Sci. 22(2):123-31.
• 37. Shivanna MB, Nagashree BR, Gurumurthy BR. 2013. In vitro response of Azadirachta indica to salinity stress and its effect of certain osmoprotectants and antioxidative enzymes. Int J Pharm Bio Sci 4:591-602.
• 38. Swapna TS. 2003. Salt stress induced changes on enzyme activities during different developments stages of rice (Oryza sativa Linn.). IJBT 2:251- 258.
• 39. Tavallali V, Rahemi M, Panahi B. 2008. Calcium induces salinity tolerance in pistachio rootstocks. Fruits 63:201-208.
• 40. Tilkat E, Işıkalan Ç, Onay A. 2005. In vitro propagation of Khinjuk Pistachio (Pistacia khinjuk Stocks) through seedling apical shoot tip culture, Propag Ornam Plants 5:124-128.
• 41. Topaloğlu K. 2010. Tuz stresinin Chili biberlerinin pigment ve kapsaisinoid değişimi ile peroksidaz aktivitesi arasındaki ilişki. Yüksek Lisans Tezi. Çukurova Üniversitesi, Adana, Türkiye.
• 42. Tourabi M. 2014. Physiological and Biochemical Responses of Plants to Salt Stress. The 1st International Conference on New Ideas in Agriculture. Islamic Azad University Khorasgan Branch, 26-27 Jan., Isfahan, Iran.
• 43. Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Ghassemi-Golezani K. 2012. Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J 5:60-67.
• 44. Yıldız M, Terzi H, Cenkci SE, Arıkan Terzi ES, Uruşak B. 2010. Physiological and Biochemical Markers of Salinity Tolerance in Plants. AUJST-A 1:1-33.
• 45. Zarinkamar F, Farjady E. 2011. Salt’s effect on anatomy and deformity of Pistacia vera nut. J Agric Sci Technol 1:599-607.