Thermal Behavior Investigation of L-Hydroxyproline Involved in Collagen Synthesis

Year 2026, Volume: 29 Issue: 1 , 1 - 12 , 08.03.2026

Ana Neacsu , Daniela Gheorghe , Ancuta Mihaela Sofronia , Cornelia Marinescu

https://doi.org/10.5541/ijot.1727178

https://izlik.org/JA44RT77SE

Abstract

In the present work, the use of various calorimetric methods (combustion calorimetry, mixing (C80), differential scanning calorimetry (DSC) and coupled method thermogravimetry-differential scanning calorimetry (TG-DSC)) were illustrated as a powerfull techniques that can provide valuable thermodynamic properties of an important component of collagen, namely L-hydroxyproline (4-hydroxy-L-proline). The thermal behavior of 4-hydroxy-L-proline was studied in the temperature range between 20 ºC to 500 °C, the transformation (melting-decomposition) point, weight loss, purity and the associated thermal effects were determined from the DSC, TG-dTG curves respectively. The values of 4-hydroxy-L-proline enthalpies of combustion and formation in solid state were calculated; information about the thermodynamic stability were obtained. Spectral characterization of the sample applying Fourier Transformed Infrared (FT-IR) method was performed. The thermal effects of the amino acid solution in water at 25 °C were calculated.

Keywords

4-hydroxy-L-proline , enthalpy of combustion and formation , thermodynamic stability , heat of solution , purity , spectral characterization

Ethical Statement

Authors approve that to the best of their knowledge, there is not any conflict of interest or common interest with an institution/organization or a person that may affect the review process of the paper.

Thanks

This contribution is carried out within the research program “Chemical Thermodynamics” of the “Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy. Support of the EU (ERDF) and Romanian Government, for the acquisition of the research infrastructure under Project INFRANANOCHEM- No.19/01.03.2009 is gratefully acknowledged.

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