Türkiye'den Fumaria asepala Boiss. ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Hausskn. 'nın Uçucu Yağ Bileşenleri ile Antimikrobiyal Aktivitesi ve Antioksidan Kapasitesinin Analizi

Abstract

Bu çalışmada, Türkiye’de yetişen Fumaria asepala Boiss. (Akşahtere) ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. (Şahtere) türlerinin uçucu yağ bileşenleri ile metanol ekstrelerinin antimikrobiyal ve antioksidan aktivitelerinin belirlenmesi amaçlanmıştır. Uçucu yağlar hidrodistilasyon yöntemi ile izole edilirken, bu yağların analizi GC/MS cihazı ile belirlenmiştir. Elde edilen sonuçlara göre F. asepala’nın uçucu yağının esas bileşeninin Phytol (%20.74) olduğu, bunu Thymol (%20.42), Benzyl Benzoate (%15.89) ve Hexahydrofarnesyl acetone (%12.92)’ ın izlediği; F. schleicheri subsp. microcarpa’nın ise uçucu yağının esas bileşeninin Benzyl Benzoate (%29.07) olduğu ve bunu sırasıyla Hexahydrofarnesyl acetone (%19.72), n-Hexadecanoic acid (%11.40) ve Phytol (%10.04)’ ün izlediği saptanmıştır. Antimikrobiyal etkilerini tespit etmek için disk difüzyon yöntemi kullanılmıştır. F. schleicheri subsp. microcarpa’nın toprak üstü kısımları en iyi antimikrobiyal etkiyi K. pneumoniae’e- ATCC 700603 (25mm) karşı gösterirken, F. asepala ise en iyi antimikrobiyal etkiyi S. aureus-ATCC 25923 (24mm) ve K. pnuemoniae’e karşı (24mm) göstermiştir. Antioksidan etkilerini belirlemek için toplam antioksidan seviyesi (TAS) ve toplam oksidan seviyesi (TOS) tespit edilmiştir. F. schleicheri subsp. microcarpa’nın 1 mg/ml konsantrasyonda metanol ekstresinin TAS değeri 2.8314 mmol olduğu, F. asepala’nın TAS değerinin aynı konsantrasyonda 3.1610 mmol olduğu tespit edilmiştir. Her iki türün toplam oksidan seviyelerinin ise yüksek değerlerde olduğu belirlenmiştir.

Keywords

• Antimikrobiyal aktivite
• antioksidan kapasite
• fumaria
• uçucu yağ

Analysis of the Essential Oil Composition, Antimicrobial Activity and Antioxidant Capacity of Fumaria asepala Boiss. and Fumaria schleicheri Soy.Will. subsp. microcarpa Hausskn. from Turkey

Abstract

This study aims to determine the antimicrobial and antioxidant activities of essential oil components and methanol extracts of Fumaria asepala Boiss. (Akşahtere) and Fumaria schleicheri Soy.-Will. subsp. microcarpa Hausskn. (Şahtere) species grown in Turkey. While essential oils were isolated by the hydrodistillation method, the analysis of these oils was determined by GC/MS device. According to the results obtained, the main component of the essential oil of F. asepala is Phytol (20.74%), followed by Thymol (20.42%), Benzyl Benzoate (15.89%), and Hexahydrofarnesyl acetone (12.92%); It was determined that the main component of the essential oil of F. schleicheri subsp. microcarpa is Benzyl Benzoate (29.07%), followed by Hexahydrofarnesyl acetone (19.72%), n-Hexadecanoic acid (11.40%) and Phytol (10.04%). The disk diffusion method was used to determine its antimicrobial effects. The above-ground parts of F. schleicheri subsp. microcarpa showed the best antimicrobial effect against K. pneumoniae-ATCC 700603 (25mm), while F. asepala showed the best antimicrobial effect against S. aureus-ATCC 25923 (24mm) and K. pneumoniae (24mm). To determine their antioxidant effects, total antioxidant level (TAS) and total oxidant level (TOS) were determined. It was determined that the TAS value of the methanol extract of F. schleicheri subsp. microcarpa at a concentration of 1 mg/ml was 2.8314 mmol, and the TOS value of F. asepala was 3.1610 mmol at the same concentration. It was determined that the total oxidant levels in both species were high.

Keywords

• Antimicrobial activity
• Antioxidant capacity
• Fumaria
• Essential oil

References

1. Adhama, A., Abedb, R., Ritsemab, C. (2021). A Reliability Analysis of Rainwater Catchment System. Water Resources, 48(3), 361–367.
2. Ashnagar, A., Gharib Naseri, N., Yaghobi, M. (2007). Chemical composition of the essential oil of fumitory plant (Fumaria parviflora Lam.) (Fineleaf fumitory) grown in Ahwaz, Iran. Asian Journal of Chemistry, 19(7), 5336-5340.
3. Avoseh, O.N., Mtunzi, F.M., Ogunwande, I.A., Ascrizzi, R., Guido, F. (2021). Albizia lebbeck and Albizia zygia volatile oils exhibit anti-nociceptive and anti-inflammatory properties in pain models. Journal of Ethnopharmacology, 268, 113676.
4. Bayaz, M. (2014). Esansiyel Yağlar: Antimikrobiyal, Antioksidan ve Antimutajenik Aktiviteleri. Akademik Gıda, 12, 45-53.
5. Baydar, H. (2005). Tıbbi, Aromatik ve Keyf Bitkileri Bilimi ve Teknolojisi. Süleyman Demirel Üniversitesi Yayınları, Isparta, 113–115.
6. Bribi, N., Algieri, F., Rodriguez-Nogales, A., Vezza, T., Garrido-Mesa, J., Utrilla, M.U., Contreras, M., Maiza, F., Segura-Carretero, A., Rodriguez-Cabezas, M.E., Galvez, J. (2016). Intestinal anti-inflammatory effects of total alkaloid extract from Fumaria capreolata in the DNBS model of mice colitis and intestinal epithelial CMT93 cells. Phytomedicine, 23: 901–913.
7. Coban, T., Citoǧlu, G., Sever, B., İscan, M. (2003). Antioxidant activities of plants used in traditional medicine in Turkey. Pharmaceutical biology, 41(8), 608-613.
8. Collins, C.M., Lyne, P.M. (2004). Microbiological methods. Oxford University Press Inc, London, 140.

9. Craker, L.E., Gardner, Z., Etter, S.C. (2003). Herbs in American fields: A horticultural perspective of herband medical plant production in the United Sates. Horticultural science, 38: 977–983.
10. Daines, A., Payne, R., Humphries, M., Abell, A. (2003). The synthesis of naturally occurring vitamin K and vitamin K analogues. Current organic chemistry, 7(16), 1625-1634.
11. Demirpolat, A., Bagci, E., Doğan, G. (2017). Essential Oil Composition of Scandix iberica Bieb. and Scandix stellata Banks and Sol. (Apiaceae) from Different Parts, a Chemotaxonomic Approach. Global journal of botanical science, 5(2), 55-62.
12. Erecevit Sönmez, P., Çakılcıoğlu, U. (2021). Türkiye’de Yetişen Centaurea urvillei DC. subsp. urvillei’ nin Antibakteriyel ve Antifungal Özelliği. Int. J. Pure Appl. Sci., 7(3), 517-525.
13. Goncalves, S., Romano, A. (2016). The medicinal potential of plants from genus Plantago (Plantaginaceae). Industrial Crops and Products, 83(1), 213-226.
14. Inci, S., Eren, A., Kirbag S. (2021). Determination of antimicrobial and antioxidant activity of Alchemilla alpina L. Turkish Journal of Agriculture-Food Science and Technology 9(12), 2260-2264.
15. Jaberian, H., Piri, K., Nazari J. (2013). Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food chemistry, 136(1), 237-244.
16. Kan, Y. (2005). Production and consumption potential of medicinal and aromatic plants in Turkey. Pharmacognosy and Phytotherapy Symposium; İstanbul, Proceedings Book, 56–61.
17. Karaaslan, N.M., Karaaslan, M.G., Ates, B. (2018). Effects of Some Extraction Solvents on the Antioxidant Properties of Strawberry Fruit. Int. J. Pure and Appl. Sci., 4 (2), 102-109.
18. Khamtache-Abderrahim, S., Lequart-Pillon, M., Gontier, E., Gaillard, I., Pilard, S., Mathiron, D., Maiza-Benabdesselam, F. (2016). Isoquinoline alkaloid fractions of Fumaria officinalis: Characterization and evaluation of their antioxidant and antibacterial activities. Industrial Crops and Products, 94, 1001-1008.
19. Kirbag, S., Erecevit, P., Zengin, F., Guvenc, A.N. (2013). Antimicrobial activities of some Euphorbia species. Afr J Tradit Complement Altern Med., 10(5), 305-309.
20. Knowles, C.O. (1991). 22.4.2 Benzyl Benzoate. In Hayes, Wayland J.; Laws, Edward R. (eds.). Handbook of Pesticide Toxicology, Classes of Pesticides. Academic Press, 3, 1505–1508.
21. Landry, B.K., Tariq, S., Malik, A., Sufyan, M., Ashfaq, U.A., Ijaz, B., Shahid, A.A. (2021). Berberis lyceum and Fumaria indica: in vitro cytotoxicity, antioxidant activity, and in silico screening of their selected phytochemicals as novel hepatitis C virus nonstructural protein 5A inhibitors. Journal of Biomolecular Structure and Dynamics, 25: 1-25.
22. Maiza, B.F., Khentache, S., Bougoffa, K., Chibane, M., Adach, S., Chapeleur, Y., Max, H., Laurain-Mattar, D. (2007). Antioxidant activities of alkaloid extracts of tow Algerian species of Fumaria: Fumaria capreolata and Fumaria bastardii. Records of Natural Products, 1: 28-35.
23. Maiza, B.F., Bribi, N. (2012). Antibacterial activity against multi resistant bacteria strains of alkaloid extracts of two Algerian Fumaria species. Microbes In Applied Research, 464-468.
24. Moghtader, M. (2013). In vitro antifungal effects of Fumaria vaillantii Loisel. essential oil on Aspergillus flavus. Journal of Yeast and Fungal Research, 4: 21–25.
25. Netscher, T. (2007). Synthesis of Vitamin E, Vitamins & Hormones, Academic Press, 76: 155-202.
26. Orhan, I., Özçelik, B., Karaoğlu, T., Şener, B. (2007). Antiviral and antimicrobial profiles of selected isoquinoline alkaloids from Fumaria and Corydalis species. Zeitschrift für Naturforschung C, 62(1-2), 19-26.
27. Orhan, I.E., Sener, B., Musharraf, S.G. (2012). Antioxidant and hepatoprotective activity appraisal of four selected Fumaria species and their total phenol and flavonoid quantities. Experimental and Toxicologic Pathology, 64, 205–209.
28. Păltinean, R., Wauters, J.N., Tits, M., Frédérich, M., Angenot, L., Tămas, M., Crisan, G. (2013). Comparative morphological studies on some species of the genus Fumaria. Farmacia, 61: 371–377.
29. Păltinean, R., Crisan, G., Balica, G., Ielciu, I., Stefănescu, C., Tămas, M. (2015). Histo-anatomical researches on the vegetative organs of five Romanian Fumaria species. Farmacia, 63: 262–266.
30. Păltinean, R., Mocan, A., Vlase, L., Gheldiu, A.M., Crișan, G., Ielciu, I., Voștinaru, O., Crișan, O. (2017). Evaluation of Polyphenolic Content, Antioxidant and Diuretic Activities of Six Fumaria Species. Molecules, 22(4), 639.
31. Pandey, M.B., Singh, A.K., Singh, A.K., Singh, U.P. (2007). Inhibitive Effect of Fuyuziphine isolated from Plant (Pittapapra) (Fumaria indica) on Spore Germination of Some Fungi. Mycobiology, 35(3), 157-158.
32. Pérez-Gutiérrez, M.A., Romero-García, A.T., Salinas, M.J., Blanca, G., Fernández, C., Suárez-Santiago, V.N. (2012). Phylogeny of the tribe Fumarieae (Papaveraceae s.l.) based on chloroplast and nuclear DNA sequences: Evolutionary and biogeographic implications. American Journal of Botany, 99: 517–528.
33. Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., Ercisli S. (2009). Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences, 22(1), 102-106.
34. Sofiane, I., Seridi, R. (2021). Phytochemical profile, total phenolic content and antioxidant activity of ethanolic extract of fumitory (Fumaria capreolata L.) from Algeria. European Journal of Biological Research, 11(4), 404-416.
35. Sousek, J., Guedon, D. (1999). Alkaloids and organic acids content of Eight Fumaria species. Phytochem Anal, 10, 6-11.
36. Stanojević, L., Zvezdanović, J., Danilović, B., Cvetković, D., Stanojević, J., Ilić, D., Cakić, M. (2018). The antioxidative and antimicrobial activity of the aqueous earth smoke (Fumaria officinalis L.) extract. Advanced Technologies, 7(2), 31-40.
37. Stuart MC, Kouimtzi M, Hill SR (eds.). (2009). World Health Organization, WHO Model Formulary p. 311. hdl:10665/44053. ISBN 9789241547659.
38. Tutin, T.G., Burges, N.A., Chater, A.O., Edmonson, J.R., Heywood, V.H., Moore, D.M., Valentine, D.H., Walters, S.M., Webb, D.A. (2010). Psilotaceae to Platanaceae In Flora Europaea. Cambridge University Press, Cambridge, UK, 2(1), 306–311.
39. Vrancheva, R., Ivanov, I., Marchev, A., Pavlov, A. (2012). Qualitative and quantitative determination of protopine in Fumaria sp. by TLC-densitometry method. Journal of BioScience and Biotechnology, 1, 255–259.
40. Vrancheva, R.Z., Ivanov, I.G., Aneva, I.Y., Dincheva, I.N., Badjakov, I.K., Pavlov, A.I. (2014). GC-MS based metabolite profiling of five Bulgarian Fumaria species. Journal of BioScience & Biotechnology, 3(3), 195-201.
41. Wei, G., Kong, L., Zhang, J., Ma, C., Wu, X., Li, X., Jiang, H. (2016). Essential oil composition and antibacterial activity of Lindera nacusua (D. Don) Merr. Natural Product Research, 30(23), 2704-2706.