Dağ Keçilerinden İzole Edilen Anaerobik Gut Fungusların Moleküler Tanımlanması

Abstract

Anaerobik Gut funguslar (AGF) Neocallimastigomycota filumunda bulunmaktadır, bu fungusların özellikle otoburların sindirim sisteminde, yemlerin tüketiminde temel koloni olduğundan dolayı önemli rol oynadığı bilinmektedir. AGF’lar salgıladıkları lig-noselülozik enzimler yardımıyla bitki dokularını parçalayarak sindirilmesini sağla-maktadırlar. Bu enzimler son yıllarda gıda ve yem sanayinde de kullanılmaktadır. Bu açıdan farklı ruminant hayvanlardan izole edilen ve tanımlaması yapılan funguslar değerli olmaktadır. Bu çalışmada Tunceli yöresinde yaban hayatta bulunan dağ keçilerinden fungus izolasyonu yapılmış, morfolojik ve moleküler olarak tanımlanmıştır. Moleküler tanımlamada yaklaşık 1500 bç uzunluğundaki ITS bölgesi kullanılmıştır. İzole edilen 9 fungustan Orpinomiyces sp. (AGF-5) Feramyces sp.(AGF-8,10) ve Neocallimastix sp. (AGF-1,2,3,4,7,11) cinslerine ait olduğu belir-lenmiştir. Yabani ruminant hayvanlardaki mikrobiyal popülasyonun belirlenmesi için daha fazla çalışmaya ihtiyaç bulunmaktadır.

Keywords

• Anaerobik Gut fungi
• Dağ keçileri
• PCR
• ITS

Molecular Identification of Anaerobic Gut Fungi Isolated from Mountain Goats

Abstract

Anaerobic Gut Fungi (AGF) are found in the Neocallimastigomycota phylum, and it is known that these fungi play an important role, especially in the digestive system of herbivores, as they are the main colonies in the consumption of feed. AGFs provide digestion by breaking down plant tissues with the help of lignocellulosic enzymes they secrete. These enzymes have also been used in the food and feed industry in recent years. In this respect, fungi isolated and identified from different ruminant animals are valuable. In this study, fungi were isolated from mountain goats living in the wild in the Tunceli region and identified morphologically and molecularly. The ITS region of ap-proximately 1500 bp was used in molecular identification. It was determined that 9 isolated fungi belonged to the genera Orpinomyces sp. (AGF-5), Feramyces sp. (AGF-8,10) and Neocallimastix sp. (AGF-1,2,3,4,7,11). Further studies are needed to determine the microbial population in wild ruminant animals.

Keywords

• Anaerobik Gut fungi
• Mountain Goats
• fungus
• PCR
• ITS

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