Green Synthesis of Glycerol-Plasticized Chitosan Biofilms Containing Macerated St. John's Wort Oil

Abstract

Abstract: In this study, St. John's Wort oil was obtained by maceration, a tradi-tional method, using olive oil macerate. St. John's Wort oil was added to chi-tosan-based biofilms as an additive, and chitosan-based biofilms containing chi-tosan/St. John's Wort oil (SJWO) were obtained. Biofilms were characterized by FTIR. The preparation and physical potential use of these films are presented. The porosity, swelling capacity, thickness, degradation rates, pore volumes and opacity of biofilms were determined. It was observed that as the amount of oil entering the structure increased, the thickness of the film increased and its swelling properties decreased. The thickness and opacity of CN/SJW-0.2, CN/SJW-0.6 and CN/SJW-0.8 biofilms were determined as 0.17, 0.28 and 0.30 mm and 1.21, 0.98 and 0.94, respectively. It was determined that increasing the amount of SJWO in-creased the film thickness and decreased its opacity. The pore volume (Vp) values of CN/SJW-0.2, CN/SJW-0.6, and CN/SJW-0.8 were calculated as 0.098, 0.0085 and 0.0086. The porosity values of the films CN/SJW-0.2, CN/SJW-0.6 and CN/SJW-0.8 were determined as 0.01952, 0.00172 and 0.00175 re-spectively.

Keywords

• maseration
• biofilm
• chitosan
• porosity
• st. john's wort oil

Maserat Sarı Kantaron Yağı İçeren Gliserol-Plastikleştirilmiş Kitosan Biyofilmlerinin Yeşil Sentezi

Abstract

Özet: Bu çalışmada, zeytinyağı maseratı kullanılarak geleneksel bir yöntem olan ma-serasyon yoluyla kantaron yağı elde edildi. Kantaron yağı, kitosan bazlı biyofilmlere katkı maddesi olarak eklenmiş ve kitosan/ kantaron yağı (SJWO) içeren kitosan bazlı biyofilmler elde edildi. Biyofilmler FTIR ile karakterize edilmiştir. Bu filmlerin hazırlanması ve fiziksel kullanım potansiyelleri sunulmuştur. Biyofilmlerin gözenekliliği, şişme kapasitesi, kalınlığı, bozunma hızları, gözenek hacimleri ve opaklığı belirlendi. Yapıya giren yağ miktarı arttıkça filmin kalınlığının arttığı ve şişme özelliklerinin azaldığı gözlendi. CN/SJW-0.2, CN/SJW-0.6 ve CN/SJW-0.8 biyofilm-lerinin kalınlığı ve opaklığı sırasıyla 0.17, 0.28 ve 0.30 mm ve 1.21, 0.98 ve 0.94 olarak belirlendi. SJWO miktarının art-masıyla film kalınlığını artırdığı ve opaklığının azaldığı belirlendi. CN/SJW-0.2, CN/SJW-0.6 ve CN/SJW-0.8'in gözenek hacmi (Vp) değerleri sırasıyla 0.098, 0.0085 ve 0.0086 olarak hesaplandı. CN/SJW-0.2, CN/SJW-0.6 ve CN/SJW-0.8 filmlerinin gözeneklilik değerleri ise sırasıyla 0.01952, 0.00172 ve 0.00175 olarak belirlendi.

Keywords

• Sarı kantaron yağı
• maserasyon
• biyofilm
• kitosan
• gözeneklilik

References

1. [1] T. Perumal, C. Krebs De Souza, T. C. Nihues, P. Jain, K. K. Gaikwad, ve S. Roy, “A review on biopolymer-based oil and water-resistant functional paper coating for food packaging”, Food Bioscience, c. 63, 105656, 2025, doi: 10.1016/j.fbio.2024.105656.
2. [2] M. Woźniak, J. Młodziejewska, K. Stefanowska, L. Mrówczyńska, A. Sip, R. Dobrucka, ve I. Ratajczak, “Chitosan-based films with essential oil components for food packaging”, Coatings, c. 14, sy 7, 830, 2024, doi: 10.3390/coatings14070830.
3. [3] M. Aider, “Chitosan application for active bio-based films production and potential in the food industry: Review”, LWT-Food Science and Technology, c. 43, sy 6, ss. 837-842, 2010, doi: 10.1016/j.lwt.2010.01.021.
4. [4] L. Li, C. Wu, Q. Chen, Z. Shi, K. Xu, Y. Niu, ve X. Rao, “Preparation of dehydroabietic acid modified chitosan/wintergreen essential oil film and mandarin freshness preservation study”, Food Chemistry, c. 464, 141836, 2025, doi: 10.1016/j.foodchem.2024.141836.
5. [5] A. Homez-Jara, L. D. Daza, D. M. Aguirre, J. A. Muñoz, J. F. Solanilla, ve H. A. Váquiro, “Characterization of chitosan edible films obtained with various polymer concentrations and drying temperatures”, International Journal of Biological Macromolecules, c. 113, ss. 1233-1240, 2018, doi: 10.1016/j.ijbiomac.2018.03.057.
6. [6] B. Azimzadeh ve M. Jahadi, “Effect of chitosan edible coating with Laurus nobilis extract on shelf life of cashew”, Food Science & Nutrition, c. 6, sy 4, ss. 871-877, 2018, doi: 10.1002/fsn3.630.
7. [7] Q. Ma, Y. Zhang, F. Critzer, P. M. Davidson, S. Zivanovic, ve Q. Zhong, “Physical, mechanical, and antimicrobial properties of chitosan films with microemulsions of cinnamon bark oil and soybean oil”, Food Hydrocolloids, c. 52, ss. 533-542, 2016, doi: 10.1016/j.foodhyd.2015.07.036.
8. [8] E. Eroglu ve S. N. Girgin, “A unique phenolic extraction method from olive oil macerate of Hypericum perforatum using DMSO: Assessment of in vitro anticancer activity, LC-MS/MS profile, total phenolic content and antioxidant capacity”, South Afr. J. Bot., c. 139, ss. 6-11, 2021, doi: 10.1016/j.sajb.2021.01.015.

9. [9] M. Jarzębski, W. Smułek, H. M. Baranowska, Ł. Masewicz, J. Kobus-Cisowska, M. Ligaj, ve E. Kaczorek, “Characterization of St. John’s wort (Hypericum perforatum L.) and the impact of filtration process on bioactive extracts incorporated into carbohydrate-based hydrogels”, Food Hydrocolloids, c. 104, 105748, 2020, doi: 10.1016/j.foodhyd.2020.105748.
10. [10] G. Aydin, N. Sirin, M. Kekecoglu, T. T. Akcay, N. Sipahi, ve H. Goksu, “The use of natural preservative propolis and Hypericum perforatum oil in herbal cream production”, IJTCMR, c. 2, sy 1, ss. 27-35, 2021.
11. [11] İ. Küçük ve N. Yıldırım, “Sarı Kantaron (Hypericum perforatum) katkılı kitosan ve jelatin esaslı yara örtücü filmlerinin üretimi ve karakterizasyonu”, GUMMFD, c. 35, sy. 1, ss. 127–136, 2019, doi: 10.17341/gazimmfd.443639.
12. [12] S. Güneş ve F. Tıhmınlıoğlu, “Hypericum perforatum incorporated chitosan films as potential bioactive wound dressing material”, International Journal of Biological Macromolecules, c. 102, ss. 933-943, 2017, doi: 10.1016/j.ijbiomac.2017.04.080.
13. [13] İ. Erdogan Orhan, M. Kartal, A. R. Gülpinar, P. Cos, A. Matheeussen, L. Maes ve D. Taşdemir, “Assessment of antimicrobial and antiprotozoal activity of the olive oil macerate samples of Hypericum perforatum and their LC-DAD-MS analyses”, Food Chemistry, c. 138, sy 2-3, ss. 870-875, 2013, doi: 10.1016/j.foodchem.2012.11.053.
14. [14] İ. Arsić, A. Žugić, D. Runjajić Antić, G. Zdunić, D. Dekanski, G. Marković ve V. Tadić, “Hypericum Perforatum L. Hypericaceae/Guttiferae sunflower, olive and palm oil extracts attenuate cold restraint stress–induced gastric lesions”, Molecules, c. 15, sy 10, ss. 6688-6698, 2010, doi: 10.3390/molecules15106688.
15. [15] J. Bonilla, L. Atarés, M. Vargas ve A. Chiralt, “Effect of essential oils and homogenization conditions on properties of chitosan-based films”, Food Hydrocolloids, c. 26, sy 1, ss. 9-16, 2012, doi: 10.1016/j.foodhyd.2011.03.015.
16. [16] L. Sánchez-González, M. Cháfer, C. González-Martínez, A. Chiralt ve S. Desobry, “Study of the release of limonene present in chitosan films enriched with bergamot oil in food simulants”, Journal of Food Engineering, c. 105, sy 1, ss. 138-143, 2011, doi: 10.1016/j.jfoodeng.2011.02.016.
17. [17] S. Bhowmik, D. Agyei ve A. Ali, “Bioactive chitosan and essential oils in sustainable active food packaging: Recent trends, mechanisms, and applications”, Food Packaging and Shelf Life, c. 34, 100962, 2022, doi: 10.1016/j.fpsl.2022.100962.
18. [18] S. Fan, O. Yang, M. Fauconnier, C. Zhu, F. Fang, X. Li, D. Zhang, A. Richel ve C. Hou, “Zein/chitosan Janus film incorporated with tannic acid and cinnamon essential oil co-loaded Pickering emulsion for sustained controlled release and pork preservation”, International Journal of Biological Macromolecules, c. 286, 138429, 2025, doi: 10.1016/j.ijbiomac.2024.138429.
19. [19] T. De Moraes Crizel, A. De Oliveira Rios, V. D. Alves, N. Bandarra, M. Moldão-Martins ve S. Hickmann Flôres, “Active food packaging prepared with chitosan and olive pomace”, Food Hydrocolloids, c. 74, ss. 139-150, 2018, doi: 10.1016/j.foodhyd.2017.08.007.
20. [20] M. Daeialiakbar, S. Yousefi ve W. Weisany, “Enhanced properties of chitosan-PVA nanocomposite films with lemongrass oil microcapsules”, Carbohydrate Polymer Technologies and Applications, c. 9, 100668, 2025, doi: 10.1016/j.carpta.2025.100668.
21. [21] R. Cai, L. Jia, R. Yang, H. Tao, H. Cui, L. Lin, E. Khojah, T. Bushnaq ve C. Shi, “Fabrication of guar gum/chitosan edible films reinforced with orange essential oil nanoemulsion for cheese preservation”, International Journal of Biological Macromolecules, c. 285, 138285, 2025, doi: 10.1016/j.ijbiomac.2024.138285.
22. [22] I. Demirhan, A. Korkmaz, E. Oner, N. Gumuscu, Y. Erbil, O. Babaarslan ve E. B. Kurutas, “Synthesis, characterization, and antibacterial effect of St. John’s wort oil loaded chitosan hydrogel”, International Journal of Biological Macromolecules, c. 260, 129444, 2024, doi: 10.1016/j.ijbiomac.2024.129444.
23. [23] M. Hadidi, S. Pouramin, F. Adinepour, S. Haghani ve S. M. Jafari, “Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities”, Carbohydrate Polymers, c. 236, 116075, 2020, doi: 10.1016/j.carbpol.2020.116075.
24. [24] Z. Li, S. Lin, S. An, L. Liu, Y. Hu ve L. Wan, “Preparation, characterization and anti-aflatoxigenic activity of chitosan packaging films incorporated with turmeric essential oil”, International Journal of Biological Macromolecules, c. 131, ss. 420-434, 2019, doi: 10.1016/j.ijbiomac.2019.02.169.
25. [25] Y. Sun, Z. Liu, X. Wang, F. Zhang, X. Huang, J. Li, X. Sun, Y. Guo ve X. Han, “Effect of HLB value on the properties of chitosan/zein/lemon essential oil film-forming emulsion and composite film”, International Journal of Food Science and Technology, c. 56, sy 10, ss. 4925-4933, 2021, doi: 10.1111/ijfs.15216.
26. [26] X. Yang, J. Liang, Q. Bao, W. Pan, Y. Wang, K. Wang, Y. Gong, H. Wu ve Q. Liu, “Preparation and characterization of polyvinyl alcohol and chitosan composite film with cassia oil encapsulated in β-cyclodextrin and its application in fresh banana”, International Journal of Biological Macromolecules, c. 301, 140246, 2025, doi: 10.1016/j.ijbiomac.2025.140246.
27. [27] Q. Peng, F. Wen, X. Tang, S. Lu, H. Li, C. Wang, Z. Chen, Z. Zhou, S. Tan, H. Qin, Y. Bi, Z. Wang ve F. Kong, “Carboxymethyl chitosan-gelatin based films filled with whey protein-stabilized nanoscale essential oil for skin wound healing: In vivo and in vitro studies”, International Journal of Biological Macromolecules, c. 305, 141119, 2025, doi: 10.1016/j.ijbiomac.2025.141119.
28. [28] X. Zhang, B. B. Ismail, H. Cheng, T. Z. Jin, M. Qian, S. A. Arabi, D. Liu ve M. Guo, “Emerging chitosan-essential oil films and coatings for food preservation: A review of advances and applications”, Carbohydrate Polymers, c. 273, 118616, 2021, doi: 10.1016/j.carbpol.2021.118616.
29. [29] F. F. Taktak ve H. N. Kaya, “Biodegradable PVA/chitosan-based films enriched with rose hip extract and seed oil: Investigation of the influence of tragacanth gum ratio on functional properties and its application in cherry preservation”, International Journal of Biological Macromolecules, c. 307, 141023, 2025, doi: 10.1016/j.ijbiomac.2025.141023.
30. [30] M. W. Apriliyani, P. Purwadi, A. Manab, M. W. Apriliyanti ve A. D. Ikhwan, “Characteristics of moisture content, swelling, opacity and transparency with addition chitosan as edible films/coating base on casein”, Advance Journal of Food Science and Technology, c. 18, sy 1, ss. 9-14, 2020, doi: 10.19026/ajfst.18.6041.
31. [31] A. Mohandas, B. S. Anisha, K. P. Chennazhi ve R. Jayakumar, “Chitosan–hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds”, Colloids and Surfaces B: Biointerfaces, c. 127, ss. 105-113, 2015, doi: 10.1016/j.colsurfb.2015.01.024.
32. [32] P. T. S. Kumar, V.-K. Lakshmanan, R. Biswas, S. V. Nair ve R. Jayakumar, “Synthesis and biological evaluation of chitin hydrogel/nano ZnO composite bandage as antibacterial wound dressing”, Journal of Biomedical Nanotechnology, c. 8, sy 6, ss. 891-900, 2012, doi: 10.1166/jbn.2012.1461.
33. [33] N. Bölgen, D. Demir, M. S. Yalçın ve S. Özdemir, “Development of Hypericum perforatum oil incorporated antimicrobial and antioxidant chitosan cryogel as a wound dressing material”, International Journal of Biological Macromolecules, c. 161, ss. 1581-1590, 2020, doi: 10.1016/j.ijbiomac.2020.08.056.