Karkamış Baraj Gölü'nde Gökkuşağı Alabalığı (Oncorhynchus mykiss) Yetiştiriciliği Yapan Işletmelerden Janthinobacterium lividum'un Izolasyonu, Identifikasyonu ve Antibiyotik Duyarlılığı

Abstract

Karkamış Baraj Gölünde faaliyet gösteren alabalık yetiştiricilik tesislerinden enfeksiyona dayalı ölüm bildirisi ile işletmelere gidildi. Hasta gökkuşağı alabalıkları klinik muayeneden geçirildi. Balıklarda yavaş hareket, yeme tepkisizlik, vücut renginde kararma, ekzoftalmus ve ara sıra vücut yüzeyinde görülen ülserler tespit edildi. Hastalık semtomu gösteren balıklardan karaciğer, dalak, böbrek, bağırsak ve deri örnekleri alındı ve hem genel hem de özel besiyerlerinde ekimler yapıldı. Bu örnekler bir inkübatörde 24°C arasında değişen sıcaklıklarda 48 saatlik bir süre boyunca inkübe edildi. Besiyerinde saflaştırılan suşlara biyokimyasal tanımlama testlerinin yanı sıra Gram-pozitif ve Gram-negatif bakterilerin tür düzeyinde tanımlanmasında kulanılan (Biolog GEN III) 94 biyokimyasal tanımlama test yapıldı. Bu testlerin sonucunda 11 Janthinobacterium lividum bakterisi tanımlandı. Disk difüzyon testi sonucunda izolatların amoksisilin, penisilin, oksitetrasiklin, gentamisin, enrofloksasin, florfenikol ve amoksisilin/klavulanik asit duyarlı, eritromisin ve kolistin sülfata ise dirençli olduğu belirlendi.

Keywords

• Karkamış Baraj Gölü
• gökkuşağı alabalığı
• Biolog GEN III
• Janthinobacterium lividum

Isolation, Identification and Antibiotic Susceptibility of Janthinobacterium lividum from Rainbow Trout (Oncorhynchus mykiss) Cultured in Karkamış Dam Lake

Abstract

Fish farms operating in the Karkamış Dam Lake were visited following reports of infection-related mortalitie. A clinical examination was conducted on sick rainbow trout. The clinical signs were observed as slow movement, unresponsiveness to food, darkening of body colour, exophthalmus and occasional ulcers on the skin. Samples were obtained from fish exhibiting symptoms of the disease and cultured on both general and special media. These samples were then incubated in an incubator at temperatures ranging from 24°C for a period of 48 hours. The media-purified strains were then subjected to 94 biochemical identification tests (Biolog GEN III) for the identification of Gram-positive and Gram-negative bacteria to species level, as well as biochemical identification tests. In this study, a total of 11 Janthinobacterium lividum isolates were identified. Antibiotic susceptibility testing using the disk diffusion method revealed that all isolates were sensitive to amoxicillin, penicillin, oxytetracycline, gentamicin, enrofloxacin, florfenicol, and amoxicillin/clavulanic acid. In contrast, resistance was observed against erythromycin and colistin sulfate.

Keywords

• Karkamış dam lake
• rainbow trout
• Biolog GEN III
• Janthinobacterium lividum

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